Mingsan Miao, Liang Guo, Jinyan Xue, Yuzhen Jia, Zhenzhen Cui and Huaixia Yang
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Activators regenerated by electron transfer for atom transfer radical polymerization (ARGET ATRP) was used as a signal amplification strategy to improve the sensitivity. The initiator modified at the end of an antibody initiates the ARGET ATRP reaction. Different fluorescence signals were designed to achieve the simultaneous detection of OTA and AFB<small><sub>1</sub></small> with limits of 426.18 and 79.55 fg mL<small><sup>−1</sup></small> for AFB<small><sub>1</sub></small> and OTA, respectively. In addition, experiments were conducted on three types of samples, and the recoveries of the two mycotoxins ranged from 87.30% to 109.50%, with relative standard deviations ranging from 0.50% to 4.92% under reproducible conditions. 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引用次数: 0
摘要
开发一种简单、快速、灵敏的同时检测霉菌毒素的技术对于确保食品和药品安全具有重要意义。本研究开发了一种具有高灵敏度和高重现性的荧光传感器,用于同时检测黄曲霉毒素 B1(AFB1)和赭曲霉毒素 A(OTA)。在该传感系统中,通过碱基互补配对原理,将 AFB1 和 OTA 合体共同固定在磁珠(MBs)表面,形成 Y 型结构,并以此作为识别探针,特异性地捕获目标物。原子转移自由基聚合的电子转移再生引发剂(ARGET ATRP)被用作信号放大策略,以提高灵敏度。抗体末端修饰的引发剂启动 ARGET ATRP 反应。设计了不同的荧光信号以实现对 OTA 和 AFB1 的同时检测,AFB1 和 OTA 的检测限分别为 426.18 和 79.55 fg mL-1。此外,还对三种样品进行了实验,在重现性良好的条件下,两种霉菌毒素的回收率为 87.30% 至 109.50%,相对标准偏差为 0.50% 至 4.92%。结果表明,所开发的适配传感器足以满足食品和药品安全领域对这两种霉菌毒素的不同监管要求,并显示出巨大的潜力。
A controllable Y-shaped DNA structure assisted aptasensor for the simultaneous detection of AFB1 and OTA based on ARGET ATRP†
The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of foods and drugs. Herein, a fluorescence aptasensor with high sensitivity and reproducibility for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. In this sensing system, AFB1 and OTA aptamers were co-immobilized on the surface of magnetic beads (MBs) to form a Y-shaped structure through the principle of complementary base pairing, and were used as recognition probes to specifically capture the target. Activators regenerated by electron transfer for atom transfer radical polymerization (ARGET ATRP) was used as a signal amplification strategy to improve the sensitivity. The initiator modified at the end of an antibody initiates the ARGET ATRP reaction. Different fluorescence signals were designed to achieve the simultaneous detection of OTA and AFB1 with limits of 426.18 and 79.55 fg mL−1 for AFB1 and OTA, respectively. In addition, experiments were conducted on three types of samples, and the recoveries of the two mycotoxins ranged from 87.30% to 109.50%, with relative standard deviations ranging from 0.50% to 4.92% under reproducible conditions. The results suggest that the developed aptasensor is sufficient to meet the different regulatory requirements of the two mycotoxins in food and drug safety and shows great potential.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices