探索铜绿假单胞菌中与γ-氨基丁酸和 5-氨基戊酸结合的溶质结合蛋白及其在激活感应激酶中的作用。

IF 3.9 3区 生物学 Q2 MICROBIOLOGY MicrobiologyOpen Pub Date : 2024-05-23 DOI:10.1002/mbo3.1415
Jean Paul Cerna-Vargas, Tino Krell
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引用次数: 0

摘要

受体激活的标准方法是将信号或信号负载的溶质结合蛋白(SBP)与传感器结构域结合。许多由 SBP 结合激活的传感器组氨酸激酶(SHKs)的编码与相应的 sbp 基因相邻。我们研究了铜绿假单胞菌 PAO1 的三个 SBPs(在 AgtS(PA0600)和 AruS(PA4982)SHKs 基因附近编码),以确定这种排列的常见程度。配体筛选和微量热研究显示,SBPs PA0602 和 PA4985 优先与 GABA 结合(KD 分别为 2.3 和 0.58 μM),其次是 5-氨基戊酸(KD 分别为 30 和 1.6 μM)和乙醇胺(KD 分别为 2.3 和 0.58 μM)。相比之下,AgtB(PA0604)只能识别 5-氨基戊酸(KD = 2.9 μM)。然而,微量热滴定法并未显示 AgtS 传感器结构域与 AgtB 或 PA0602 有任何结合,无论是否存在配体。同样,细菌双杂交试验也没有证明 PA4985 与 AruS 传感器结构域之间存在相互作用。因此,位于附近的 sbp 和 shk 基因在功能上并不总是相关联的。我们之前发现 PA0222 是一种 GABA 特异性 SBP。GABA 的三个 SBP 的存在可能与 GABA 作为铜绿假单胞菌毒力触发器的作用有关。
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Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases

The standard method of receptor activation involves the binding of signals or signal-loaded solute binding proteins (SBPs) to sensor domains. Many sensor histidine kinases (SHKs), which are activated by SBP binding, are encoded adjacent to their corresponding sbp gene. We examined three SBPs of Pseudomonas aeruginosa PAO1, encoded near the genes for the AgtS (PA0600) and AruS (PA4982) SHKs, to determine how common this arrangement is. Ligand screening and microcalorimetric studies revealed that the SBPs PA0602 and PA4985 preferentially bind to GABA (KD = 2.3 and 0.58 μM, respectively), followed by 5-aminovalerate (KD = 30 and 1.6 μM, respectively) and ethanoldiamine (KD = 2.3 and 0.58 μM, respectively). In contrast, AgtB (PA0604) exclusively recognizes 5-aminovaleric acid (KD = 2.9 μM). However, microcalorimetric titrations did not show any binding between the AgtS sensor domain and AgtB or PA0602, regardless of the presence of ligands. Similarly, bacterial two-hybrid assays did not demonstrate an interaction between PA4985 and the AruS sensor domain. Therefore, sbp and shk genes located nearby are not always functionally linked. We previously identified PA0222 as a GABA-specific SBP. The presence of three SBPs for GABA may be linked to GABA's role as a trigger for P. aeruginosa virulence.

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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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