AMPK 可抑制大鼠绒毛膜细胞中的电压门控钙通道电流。

IF 3.8 3区 医学 Q2 CELL BIOLOGY Molecular and Cellular Endocrinology Pub Date : 2024-05-20 DOI:10.1016/j.mce.2024.112275
A.K. Fukumoto-Inukai , K. Bermeo , I. Arenas , M.J. Rosendo-Pineda , J.A. Pimentel-Cabrera , D.E. Garcia
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引用次数: 0

摘要

代谢变化对中枢和外周神经内分泌细胞的 Ca2+ 流入调节至关重要。为了研究 AMPK 对 L 型 Ca2+ 通道的调控,我们在大鼠绒毛膜细胞中使用了生化试剂和 ATP/葡萄糖浓度操作。低浓度的 AICAR 和化合物-C 能显著诱导 L 型 Ca2+ 通道电流振幅和电压依赖性的变化。值得注意的是,降低细胞内的 ATP 水平可诱导通道电流密度的重叠下降。相应地,随着细胞外葡萄糖浓度的降低,Ca2+ 通道电流密度也会逐渐降低。通过免疫荧光法,在降低细胞外葡萄糖浓度的同时,观察到 CaV1.2 的表达减少,这表明 AMPK 减少了进入质膜的功能性 Ca2+ 通道的数量。这些结果首次证明了 AMPK 在维持 Ca2+ 通道电流时对代谢变化的依赖性。它们揭示了分泌细胞中 Ca2+ 流入的一个关键步骤。
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AMPK inhibits voltage-gated calcium channel-current in rat chromaffin cells

Metabolic changes are critical in the regulation of Ca2+ influx in central and peripheral neuroendocrine cells. To study the regulation of L-type Ca2+ channels by AMPK we used biochemical reagents and ATP/glucose-concentration manipulations in rat chromaffin cells. AICAR and Compound-C, at low concentration, significantly induce changes in L-type Ca2+ channel-current amplitude and voltage dependence. Remarkably, an overlasting decrease in the channel-current density can be induced by lowering the intracellular level of ATP. Accordingly, Ca2+ channel-current density gradually diminishes by decreasing the extracellular glucose concentration. By using immunofluorescence, a decrease in the expression of CaV1.2 is observed while decreasing extracellular glucose, suggesting that AMPK reduces the number of functional Ca2+ channels into the plasma membrane. Together, these results support for the first time the dependence of metabolic changes in the maintenance of Ca2+ channel-current by AMPK. They reveal a key step in Ca2+ influx in secretory cells.

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来源期刊
Molecular and Cellular Endocrinology
Molecular and Cellular Endocrinology 医学-内分泌学与代谢
CiteScore
9.00
自引率
2.40%
发文量
174
审稿时长
42 days
期刊介绍: Molecular and Cellular Endocrinology was established in 1974 to meet the demand for integrated publication on all aspects related to the genetic and biochemical effects, synthesis and secretions of extracellular signals (hormones, neurotransmitters, etc.) and to the understanding of cellular regulatory mechanisms involved in hormonal control.
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