A.K. Fukumoto-Inukai , K. Bermeo , I. Arenas , M.J. Rosendo-Pineda , J.A. Pimentel-Cabrera , D.E. Garcia
{"title":"AMPK 可抑制大鼠绒毛膜细胞中的电压门控钙通道电流。","authors":"A.K. Fukumoto-Inukai , K. Bermeo , I. Arenas , M.J. Rosendo-Pineda , J.A. Pimentel-Cabrera , D.E. Garcia","doi":"10.1016/j.mce.2024.112275","DOIUrl":null,"url":null,"abstract":"<div><p>Metabolic changes are critical in the regulation of Ca<sup>2+</sup> influx in central and peripheral neuroendocrine cells. To study the regulation of L-type Ca<sup>2+</sup> channels by AMPK we used biochemical reagents and ATP/glucose-concentration manipulations in rat chromaffin cells. AICAR and Compound-C, at low concentration, significantly induce changes in L-type Ca<sup>2+</sup> channel-current amplitude and voltage dependence. Remarkably, an overlasting decrease in the channel-current density can be induced by lowering the intracellular level of ATP. Accordingly, Ca<sup>2+</sup> channel-current density gradually diminishes by decreasing the extracellular glucose concentration. By using immunofluorescence, a decrease in the expression of Ca<sub>V</sub>1.2 is observed while decreasing extracellular glucose, suggesting that AMPK reduces the number of functional Ca<sup>2+</sup> channels into the plasma membrane. Together, these results support for the first time the dependence of metabolic changes in the maintenance of Ca<sup>2+</sup> channel-current by AMPK. They reveal a key step in Ca<sup>2+</sup> influx in secretory cells.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"AMPK inhibits voltage-gated calcium channel-current in rat chromaffin cells\",\"authors\":\"A.K. Fukumoto-Inukai , K. Bermeo , I. Arenas , M.J. Rosendo-Pineda , J.A. Pimentel-Cabrera , D.E. Garcia\",\"doi\":\"10.1016/j.mce.2024.112275\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Metabolic changes are critical in the regulation of Ca<sup>2+</sup> influx in central and peripheral neuroendocrine cells. To study the regulation of L-type Ca<sup>2+</sup> channels by AMPK we used biochemical reagents and ATP/glucose-concentration manipulations in rat chromaffin cells. AICAR and Compound-C, at low concentration, significantly induce changes in L-type Ca<sup>2+</sup> channel-current amplitude and voltage dependence. Remarkably, an overlasting decrease in the channel-current density can be induced by lowering the intracellular level of ATP. Accordingly, Ca<sup>2+</sup> channel-current density gradually diminishes by decreasing the extracellular glucose concentration. By using immunofluorescence, a decrease in the expression of Ca<sub>V</sub>1.2 is observed while decreasing extracellular glucose, suggesting that AMPK reduces the number of functional Ca<sup>2+</sup> channels into the plasma membrane. Together, these results support for the first time the dependence of metabolic changes in the maintenance of Ca<sup>2+</sup> channel-current by AMPK. They reveal a key step in Ca<sup>2+</sup> influx in secretory cells.</p></div>\",\"PeriodicalId\":18707,\"journal\":{\"name\":\"Molecular and Cellular Endocrinology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular and Cellular Endocrinology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S030372072400131X\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular and Cellular Endocrinology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S030372072400131X","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
AMPK inhibits voltage-gated calcium channel-current in rat chromaffin cells
Metabolic changes are critical in the regulation of Ca2+ influx in central and peripheral neuroendocrine cells. To study the regulation of L-type Ca2+ channels by AMPK we used biochemical reagents and ATP/glucose-concentration manipulations in rat chromaffin cells. AICAR and Compound-C, at low concentration, significantly induce changes in L-type Ca2+ channel-current amplitude and voltage dependence. Remarkably, an overlasting decrease in the channel-current density can be induced by lowering the intracellular level of ATP. Accordingly, Ca2+ channel-current density gradually diminishes by decreasing the extracellular glucose concentration. By using immunofluorescence, a decrease in the expression of CaV1.2 is observed while decreasing extracellular glucose, suggesting that AMPK reduces the number of functional Ca2+ channels into the plasma membrane. Together, these results support for the first time the dependence of metabolic changes in the maintenance of Ca2+ channel-current by AMPK. They reveal a key step in Ca2+ influx in secretory cells.
期刊介绍:
Molecular and Cellular Endocrinology was established in 1974 to meet the demand for integrated publication on all aspects related to the genetic and biochemical effects, synthesis and secretions of extracellular signals (hormones, neurotransmitters, etc.) and to the understanding of cellular regulatory mechanisms involved in hormonal control.