{"title":"大规模公共数据库和转移性乳腺癌实验验证揭示的潜在生物标记物--钙传感受体","authors":"Wanlin Xie, Huimin Xu, Yangyang Cheng, Xin Lin, Jingya Zeng, Yihua Sun","doi":"10.1177/15330338241254219","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Breast cancer (BC) is a common cancer characterized by a high molecular heterogeneity. Therefore, understanding its biological properties and developing effective treatments for patients with different molecular features is imperative. Calcium-sensing receptor (CaSR) has been implicated in several regulatory functions in various types of human cancers. However, its underlying pathological mechanism in BC progression remains elusive.</p><p><strong>Methods: </strong>We utilized The Cancer Genome Atlas and Gene Expression Omnibus databases to explore the function of CaSR in the metastasis of BC. Gene ontology analysis, Kyoto Encyclopedia of Genes and Genomes analysis, and Gene Set Enrichment Analysis of biological processes and cell signaling pathways revealed that CaSR could be activated or inhibited. Importantly, quantitative reverse transcriptase-polymerase chain reaction and western blotting were used to verify the gene expression of the CaSR. Wound healing and transwell assays were conducted to assess the effect of CaSR on the migration of BC cells.</p><p><strong>Results: </strong>We demonstrated that CaSR expression in metastatic BC was higher than that in non-metastatic BC. It is the first time that database information has been used to reveal the biological process and molecular mechanism of CaSR in BC. Moreover, the CaSR expression in normal breast epithelial cells was notably less compared to that in BC cells. The activation of CaSR by Cinacalcet (a CaSR agonist) significantly enhanced the migration of BC cells, whereas NPS-2143 (a CaSR antagonist) treatment dramatically inhibited these effects.</p><p><strong>Conclusion and future perspective: </strong>Bioinformatics techniques and experiments demonstrated the involvement of CaSR in BC metastasis. Our findings shed new light on the receptor therapy and molecular pathogenesis of BC, and emphasize the crucial function of CaSR, facilitating the metastasis of BC.</p>","PeriodicalId":22203,"journal":{"name":"Technology in Cancer Research & Treatment","volume":"23 ","pages":"15330338241254219"},"PeriodicalIF":2.7000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11119385/pdf/","citationCount":"0","resultStr":"{\"title\":\"Calcium-sensing Receptor, a Potential Biomarker Revealed by Large-scale Public Databases and Experimental Verification in Metastatic Breast Cancer.\",\"authors\":\"Wanlin Xie, Huimin Xu, Yangyang Cheng, Xin Lin, Jingya Zeng, Yihua Sun\",\"doi\":\"10.1177/15330338241254219\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Breast cancer (BC) is a common cancer characterized by a high molecular heterogeneity. 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Wound healing and transwell assays were conducted to assess the effect of CaSR on the migration of BC cells.</p><p><strong>Results: </strong>We demonstrated that CaSR expression in metastatic BC was higher than that in non-metastatic BC. It is the first time that database information has been used to reveal the biological process and molecular mechanism of CaSR in BC. Moreover, the CaSR expression in normal breast epithelial cells was notably less compared to that in BC cells. The activation of CaSR by Cinacalcet (a CaSR agonist) significantly enhanced the migration of BC cells, whereas NPS-2143 (a CaSR antagonist) treatment dramatically inhibited these effects.</p><p><strong>Conclusion and future perspective: </strong>Bioinformatics techniques and experiments demonstrated the involvement of CaSR in BC metastasis. 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引用次数: 0
摘要
简介乳腺癌(BC)是一种常见的癌症,具有高度的分子异质性。因此,了解其生物学特性并针对不同分子特征的患者开发有效的治疗方法势在必行。钙传感受体(CaSR)在多种类型的人类癌症中被认为具有多种调控功能。然而,其在 BC 进展过程中的潜在病理机制仍不明确:方法:我们利用癌症基因组图谱(The Cancer Genome Atlas)和基因表达总库(Gene Expression Omnibus)数据库来探讨 CaSR 在 BC 转移中的功能。基因本体分析、京都基因和基因组百科全书分析以及生物过程和细胞信号通路的基因组富集分析表明,CaSR可被激活或抑制。重要的是,逆转录酶聚合酶链式反应定量分析和免疫印迹分析证实了 CaSR 的基因表达。我们还进行了伤口愈合和透孔试验,以评估 CaSR 对 BC 细胞迁移的影响:结果:我们发现转移性 BC 中 CaSR 的表达高于非转移性 BC。这是首次利用数据库信息揭示 CaSR 在 BC 中的生物学过程和分子机制。此外,正常乳腺上皮细胞中 CaSR 的表达明显低于 BC 细胞。Cinacalcet(一种CaSR激动剂)对CaSR的激活显著增强了BC细胞的迁移,而NPS-2143(一种CaSR拮抗剂)则显著抑制了这些效应:生物信息学技术和实验证明了 CaSR 参与了 BC 的转移。我们的发现为BC的受体治疗和分子发病机制提供了新的思路,并强调了CaSR促进BC转移的关键功能。
Calcium-sensing Receptor, a Potential Biomarker Revealed by Large-scale Public Databases and Experimental Verification in Metastatic Breast Cancer.
Introduction: Breast cancer (BC) is a common cancer characterized by a high molecular heterogeneity. Therefore, understanding its biological properties and developing effective treatments for patients with different molecular features is imperative. Calcium-sensing receptor (CaSR) has been implicated in several regulatory functions in various types of human cancers. However, its underlying pathological mechanism in BC progression remains elusive.
Methods: We utilized The Cancer Genome Atlas and Gene Expression Omnibus databases to explore the function of CaSR in the metastasis of BC. Gene ontology analysis, Kyoto Encyclopedia of Genes and Genomes analysis, and Gene Set Enrichment Analysis of biological processes and cell signaling pathways revealed that CaSR could be activated or inhibited. Importantly, quantitative reverse transcriptase-polymerase chain reaction and western blotting were used to verify the gene expression of the CaSR. Wound healing and transwell assays were conducted to assess the effect of CaSR on the migration of BC cells.
Results: We demonstrated that CaSR expression in metastatic BC was higher than that in non-metastatic BC. It is the first time that database information has been used to reveal the biological process and molecular mechanism of CaSR in BC. Moreover, the CaSR expression in normal breast epithelial cells was notably less compared to that in BC cells. The activation of CaSR by Cinacalcet (a CaSR agonist) significantly enhanced the migration of BC cells, whereas NPS-2143 (a CaSR antagonist) treatment dramatically inhibited these effects.
Conclusion and future perspective: Bioinformatics techniques and experiments demonstrated the involvement of CaSR in BC metastasis. Our findings shed new light on the receptor therapy and molecular pathogenesis of BC, and emphasize the crucial function of CaSR, facilitating the metastasis of BC.
期刊介绍:
Technology in Cancer Research & Treatment (TCRT) is a JCR-ranked, broad-spectrum, open access, peer-reviewed publication whose aim is to provide researchers and clinicians with a platform to share and discuss developments in the prevention, diagnosis, treatment, and monitoring of cancer.