通过延时成像观察胚胎形态动力学发育与附睾精子卵胞浆内注射之间的关系。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-05-24 DOI:10.1002/mrd.23747
Edson Borges Jr., Daniela Braga, Rodrigo Provenza, Assumpto Iaconelli Jr., Amanda Setti
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引用次数: 0

摘要

本研究旨在通过考虑数据聚类(每个患者的多个胚胎具有相似的发育时间),研究精子来源对胚胎形态动力学和卵胞浆内单精子显微注射(ICSI)周期临床结果的影响。这项匹配队列研究是在一所私立大学附属体外受精中心进行的。在2019年1月至2020年12月期间接受附睾精子卵胞浆内单精子显微注射的女性(经皮附睾精子抽吸组,n = 32个周期)与因特发性男性因素不育(男性因素不育[MFI]组,n = 32个周期)或女性不育(对照组,n = 32个周期)而接受射精精子卵胞浆内单精子卵胞浆内单精子显微注射的女性进行了配对。胚胎在延时成像培养箱中培养,记录形态发生过程并在各组间进行比较。从附睾精子中提取的胚胎的分裂速度明显慢于从 MFI 组和对照组中提取的胚胎。从附睾精子中提取的胚胎的 KIDScore(3.1 ± 0.2)明显低于从射精精子中提取的胚胎(5.4 ± 0.1)和对照组(5.6 ± 0.2,p<0.05)。
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Association between embryo morphokinetic development and intracytoplasmic sperm injection with epididymal sperm via time-lapse imaging

The objective of this study was to investigate the impact of sperm source on embryo morphokinetics and the clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles by considering the clustering of data (multiple embryos per patient that share a comparable developmental timing). This matched cohort study was performed at a private university–affiliated in vitro fertilization center. Women who underwent ICSI with epididymal sperm between January 2019 and December 2020 (the percutaneous epididymal sperm aspiration group, n = 32 cycles) were matched with women who underwent ICSI with ejaculated sperm because of idiopathic male factor infertility (the male factor infertility [MFI] group, n = 32 cycles) or female infertility (the control group, n = 32 cycles). Embryos were cultured in a time-lapse imaging incubator, and morphokinetic development was recorded and compared among the groups. Significantly slower divisions were observed in embryos derived from epididymal sperm than in those derived from the MFI and control groups. Embryos derived from epididymal sperm had a significantly lower KIDScore (3.1 ± 0.2) than did those derived from ejaculated spermatozoa from the MFI (5.4 ± 0.1) and control (5.6 ± 0.2, p < 0.001) groups. Epididymal sperm-derived embryos showed a significantly greater occurrence of multinucleation (23.2%) than did those derived from ejaculated sperm from the MFI and control groups (2.8% and 3.7%, p < 0.001, respectively). Epididymal sperm-derived embryos were significantly more likely to undergo direct or reverse cleavage (11.1%) than ejaculated sperm-derived embryos in the control group (4.3%, p = 0.001). In conclusion, delayed cell cleavage and increased incidences of blastomere multinucleation and abnormal cleavage patterns are observed when epididymal-derived sperm are used for ICSI.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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