ZEB1-AS1通过靶向miR-320b/BMPR1A轴介导肺癌骨转移

IF 1.9 4区 医学 Q3 RESPIRATORY SYSTEM Clinical Respiratory Journal Pub Date : 2024-05-23 DOI:10.1111/crj.13770
Nianxi Tan, Junyi Tang, Guang Chen, Weilin Jiang, Zhiqin Liu
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引用次数: 0

摘要

目的:探讨lncRNA ZEB1-AS1在肺癌中的作用和调控机制:本研究旨在探讨lncRNA ZEB1-AS1在肺癌中的作用及调控机制:方法:通过 qRT-PCR 检测 ZEB1-AS1 和 miR-320b 的表达。采用 CCK-8、集落形成和 Transwell 试验评估细胞活力、增殖迁移和侵袭。采用 Western 印迹法对 EMT 标记进行定量。在肺癌小鼠模型中评估了皮下肿瘤和转移性骨肿瘤的生长情况。此外,还使用 H&E 染色法检测了转移性骨肿瘤:结果:肺癌中 ZEB1-AS1 表达上调,而 miR-320b 水平下调。敲除 ZEB1-AS1 能显著抑制 A549 细胞的活力、增殖、迁移、侵袭和 EMT。此外,我们还证实了ZEB1-AS1与miR-320b以及miR-320b与BMPR1A之间的靶向关系。我们的研究结果表明,ZEB1-AS1通过靶向miR-320b/BMPR1A轴调节肺癌细胞的活力、增殖、迁移和侵袭以及EMT。此外,我们的体内实验证实,ZEB1-AS1通过靶向miR-320b/BMPR1A轴介导了肺癌小鼠的骨转移:结论:ZEB1-AS1通过靶向miR-320b/BMPR1A轴介导肺癌骨转移。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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ZEB1-AS1 mediates bone metastasis through targeting miR-320b/BMPR1A axis in lung cancer

Objective

This study aimed to explore the role and regulatory mechanism of lncRNA ZEB1-AS1 in lung cancer.

Methods

The expression of ZEB1-AS1 and miR-320b was determined by qRT-PCR. Cell viability, proliferation migration, and invasion were assessed using the CCK-8, colony-forming, and Transwell assay. EMT markers were quantified using western blot. The growth of subcutaneous tumor growth and metastatic bone tumors was evaluated in mouse model of lung cancer. Additionally, metastatic bone tumors were examined using H&E staining.

Results

ZEB1-AS1 expression was upregulated, while miR-320b levels were downregulated in lung cancer. Knockdown of ZEB1-AS1 resulted in a significant suppression of cell viability, proliferation, migration, invasion, and EMT in A549 cells. Furthermore, we confirmed the targeting relationship between ZEB1-AS1 and miR-320b, as well as between miR-320b and BMPR1A. Our findings suggested that ZEB1-AS1 regulated cell viability, proliferation, migration, and invasion, as well as EMT, in lung cancer cells by targeting the miR-320b/BMPR1A axis. Moreover, our in vivo experiments confirmed that ZEB1-AS1 mediated bone metastasis through targeting miR-320b/BMPR1A axis in mice with lung cancer.

Conclusion

ZEB1-AS1 mediated bone metastasis through targeting miR-320b/BMPR1A axis in lung cancer.

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来源期刊
Clinical Respiratory Journal
Clinical Respiratory Journal 医学-呼吸系统
CiteScore
3.70
自引率
0.00%
发文量
104
审稿时长
>12 weeks
期刊介绍: Overview Effective with the 2016 volume, this journal will be published in an online-only format. Aims and Scope The Clinical Respiratory Journal (CRJ) provides a forum for clinical research in all areas of respiratory medicine from clinical lung disease to basic research relevant to the clinic. We publish original research, review articles, case studies, editorials and book reviews in all areas of clinical lung disease including: Asthma Allergy COPD Non-invasive ventilation Sleep related breathing disorders Interstitial lung diseases Lung cancer Clinical genetics Rhinitis Airway and lung infection Epidemiology Pediatrics CRJ provides a fast-track service for selected Phase II and Phase III trial studies. Keywords Clinical Respiratory Journal, respiratory, pulmonary, medicine, clinical, lung disease, Abstracting and Indexing Information Academic Search (EBSCO Publishing) Academic Search Alumni Edition (EBSCO Publishing) Embase (Elsevier) Health & Medical Collection (ProQuest) Health Research Premium Collection (ProQuest) HEED: Health Economic Evaluations Database (Wiley-Blackwell) Hospital Premium Collection (ProQuest) Journal Citation Reports/Science Edition (Clarivate Analytics) MEDLINE/PubMed (NLM) ProQuest Central (ProQuest) Science Citation Index Expanded (Clarivate Analytics) SCOPUS (Elsevier)
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