抗CD20疗法对多发性硬化症患者循环和鞘内滤泡辅助T细胞亚群的影响。

IF 4.5 3区 医学 Q2 IMMUNOLOGY Clinical immunology Pub Date : 2024-05-23 DOI:10.1016/j.clim.2024.110262
Sahla El Mahdaoui , Marie Mathilde Hansen , Malene Bredahl Hansen , Victoria Hyslop Hvalkof , Helle Bach Søndergaard , Mie Reith Mahler , Jeppe Romme Christensen , Finn Sellebjerg , Marina Rode von Essen
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引用次数: 0

摘要

滤泡辅助T细胞(Tfh)及其与B细胞的相互作用可能是复发缓解型多发性硬化症(RRMS)的发病机制之一。Tfh细胞在RRMS患者的脑脊液(CSF)中富集,但抗CD20治疗的效果尚不清楚。我们使用流式细胞术研究了对照组、未接受治疗和接受抗 CD20 治疗的 RRMS 患者的 Tfh 细胞。在未经治疗的患者中,CSF Tfh细胞有所增加。与配对血液样本相比,CD25- Tfh细胞在RRMS患者的CSF中富集,而在对照组中则没有富集。在未经治疗的RRMS患者中,对比增强的脑MRI病变和IgG指数与CSF中CD25- Tfh细胞的频率相关。抗CD20疗法减少了循环中PD1+ Tfh细胞和CD25- Tfh细胞的数量,也降低了CSF CD25- Tfh细胞的频率。该研究表明,CD25- Tfh细胞被招募到RRMS的CSF中,与病灶炎症有关,并通过抗CD20治疗而减少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Effects of anti-CD20 therapy on circulating and intrathecal follicular helper T cell subsets in multiple sclerosis

Follicular helper T (Tfh) cells and their interplay with B cells likely contribute to the pathogenesis of relapsing-remitting multiple sclerosis (RRMS). Tfh cells are enriched in cerebrospinal fluid (CSF) in RRMS, but effects of anti-CD20 therapy are unknown. We investigated Tfh cells in controls, untreated and anti-CD20-treated patients with RRMS using flow cytometry. CSF Tfh cells were increased in untreated patients. Compared to paired blood samples, CD25 Tfh cells were enriched in CSF in RRMS, but not in controls. Contrast-enhancing brain MRI lesions and IgG index correlated with CSF CD25 Tfh cell frequency in untreated patients with RRMS. Anti-CD20 therapy reduced the numbers of circulating PD1+ Tfh cells and CD25 Tfh cells, and the frequency of CSF CD25 Tfh cells. The study suggests that CD25 Tfh cells are recruited to the CSF in RRMS, associated with focal inflammation, and are reduced by anti-CD20 therapy.

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来源期刊
Clinical immunology
Clinical immunology 医学-免疫学
CiteScore
12.30
自引率
1.20%
发文量
212
审稿时长
34 days
期刊介绍: Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.
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