无障碍高速图像激活细胞分拣。

IF 13 1区 生物学 Q1 CELL BIOLOGY Trends in Cell Biology Pub Date : 2024-08-01 Epub Date: 2024-05-23 DOI:10.1016/j.tcb.2024.04.007
Terra M Kuhn, Malte Paulsen, Sara Cuylen-Haering
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引用次数: 0

摘要

在过去的六十年里,荧光激活细胞分拣(FACS)通过高通量分离感兴趣的细胞,已成为基础和临床研究的一项重要技术。最近的技术进步开启了流式细胞仪的新时代。通过将显微镜的空间分辨率与高速细胞分拣相结合,新仪器可根据简单的图像衍生参数或复杂的图像分析算法进行细胞分拣,从而大大扩展了应用范围。在这篇综述中,我们将讨论市场上已有的系统,或在方法和工程方面有足够详细描述的系统,以便进行复制。我们总结了它们的优势和局限性,并重点介绍了有可能改变基础生命科学研究和临床环境中各个领域的应用。
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Accessible high-speed image-activated cell sorting.

Over the past six decades, fluorescence-activated cell sorting (FACS) has become an essential technology for basic and clinical research by enabling the isolation of cells of interest in high throughput. Recent technological advancements have started a new era of flow cytometry. By combining the spatial resolution of microscopy with high-speed cell sorting, new instruments allow cell sorting based on simple image-derived parameters or sophisticated image analysis algorithms, thereby greatly expanding the scope of applications. In this review, we discuss the systems that are commercially available or have been described in enough methodological and engineering detail to allow their replication. We summarize their strengths and limitations and highlight applications that have the potential to transform various fields in basic life science research and clinical settings.

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来源期刊
Trends in Cell Biology
Trends in Cell Biology 生物-细胞生物学
CiteScore
32.00
自引率
0.50%
发文量
160
审稿时长
61 days
期刊介绍: Trends in Cell Biology stands as a prominent review journal in molecular and cell biology. Monthly review articles track the current breadth and depth of research in cell biology, reporting on emerging developments and integrating various methods, disciplines, and principles. Beyond Reviews, the journal features Opinion articles that follow trends, offer innovative ideas, and provide insights into the implications of new developments, suggesting future directions. All articles are commissioned from leading scientists and undergo rigorous peer-review to ensure balance and accuracy.
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