Lei He , Feiteng Sun , Yunhao Wu , Zhiran Li , Yangbo Fu, Qiuru Huang, Jiaxin Li, Zihan Wang, Jiaying Cai, Chenrui Feng, Xiaonan Deng, Han Gu, Xuxin He, Jun Yu, Fei Sun
{"title":"L(1)10Bb通过睾丸干细胞龛内的细胞非自主效应,成为体细胞与胚系沟通的保守决定因素","authors":"Lei He , Feiteng Sun , Yunhao Wu , Zhiran Li , Yangbo Fu, Qiuru Huang, Jiaxin Li, Zihan Wang, Jiaying Cai, Chenrui Feng, Xiaonan Deng, Han Gu, Xuxin He, Jun Yu, Fei Sun","doi":"10.1016/j.mce.2024.112278","DOIUrl":null,"url":null,"abstract":"<div><p>The testicular stem cell niche is the central regulator of spermatogenesis in <em>Drosophila melanogaster</em>. However, the underlying regulatory mechanisms are unclear. This study demonstrated the crucial role of <em>lethal (1)</em> 10Bb [<em>l(1)</em>10Bb] in regulating the testicular stem cell niche. Dysfunction of <em>l(1)</em>10Bb in early-stage cyst cells led to male fertility disorders and compromised cyst stem cell maintenance. Moreover, the dysfunction of <em>l(1)</em>10Bb in early-stage cyst cells exerted non-autonomous effects on germline stem cell differentiation, independently of hub signals. Notably, our study highlights the rescue of testicular defects through ectopic expression of L(1)10Bb and the human homologous protein BUD31 homolog (BUD31). In addition, <em>l(1)</em>10Bb dysfunction in early-stage cyst cells downregulated the expression of spliceosome subunits in the Sm and the precursor RNA processing complexes. Collectively, our findings established <em>l(1)</em>10Bb as a pivotal factor in the modulation of <em>Drosophila</em> soma-germline communications within the testicular stem cell niche.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"L(1)10Bb serves as a conservative determinant for soma-germline communications via cellular non-autonomous effects within the testicular stem cell niche\",\"authors\":\"Lei He , Feiteng Sun , Yunhao Wu , Zhiran Li , Yangbo Fu, Qiuru Huang, Jiaxin Li, Zihan Wang, Jiaying Cai, Chenrui Feng, Xiaonan Deng, Han Gu, Xuxin He, Jun Yu, Fei Sun\",\"doi\":\"10.1016/j.mce.2024.112278\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The testicular stem cell niche is the central regulator of spermatogenesis in <em>Drosophila melanogaster</em>. However, the underlying regulatory mechanisms are unclear. This study demonstrated the crucial role of <em>lethal (1)</em> 10Bb [<em>l(1)</em>10Bb] in regulating the testicular stem cell niche. Dysfunction of <em>l(1)</em>10Bb in early-stage cyst cells led to male fertility disorders and compromised cyst stem cell maintenance. Moreover, the dysfunction of <em>l(1)</em>10Bb in early-stage cyst cells exerted non-autonomous effects on germline stem cell differentiation, independently of hub signals. Notably, our study highlights the rescue of testicular defects through ectopic expression of L(1)10Bb and the human homologous protein BUD31 homolog (BUD31). In addition, <em>l(1)</em>10Bb dysfunction in early-stage cyst cells downregulated the expression of spliceosome subunits in the Sm and the precursor RNA processing complexes. Collectively, our findings established <em>l(1)</em>10Bb as a pivotal factor in the modulation of <em>Drosophila</em> soma-germline communications within the testicular stem cell niche.</p></div>\",\"PeriodicalId\":18707,\"journal\":{\"name\":\"Molecular and Cellular Endocrinology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-05-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular and Cellular Endocrinology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0303720724001345\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular and Cellular Endocrinology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0303720724001345","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
L(1)10Bb serves as a conservative determinant for soma-germline communications via cellular non-autonomous effects within the testicular stem cell niche
The testicular stem cell niche is the central regulator of spermatogenesis in Drosophila melanogaster. However, the underlying regulatory mechanisms are unclear. This study demonstrated the crucial role of lethal (1) 10Bb [l(1)10Bb] in regulating the testicular stem cell niche. Dysfunction of l(1)10Bb in early-stage cyst cells led to male fertility disorders and compromised cyst stem cell maintenance. Moreover, the dysfunction of l(1)10Bb in early-stage cyst cells exerted non-autonomous effects on germline stem cell differentiation, independently of hub signals. Notably, our study highlights the rescue of testicular defects through ectopic expression of L(1)10Bb and the human homologous protein BUD31 homolog (BUD31). In addition, l(1)10Bb dysfunction in early-stage cyst cells downregulated the expression of spliceosome subunits in the Sm and the precursor RNA processing complexes. Collectively, our findings established l(1)10Bb as a pivotal factor in the modulation of Drosophila soma-germline communications within the testicular stem cell niche.
期刊介绍:
Molecular and Cellular Endocrinology was established in 1974 to meet the demand for integrated publication on all aspects related to the genetic and biochemical effects, synthesis and secretions of extracellular signals (hormones, neurotransmitters, etc.) and to the understanding of cellular regulatory mechanisms involved in hormonal control.