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引用次数: 0
摘要
摘要 一系列重组单克隆抗体（rMAbs）已被用于治疗各种疾病，包括各种癌症和免疫系统疾病。中国仓鼠卵巢（CHO）细胞因其稳健性、易转染性以及与人体细胞相似的翻译后修饰能力，已成为生产这些 rMAbs 的主要选择。瞬时转染和/或稳定表达可用于在 CHO 细胞中表达 rMAbs。为提高 CHO 细胞中 rMAbs 的产量，已开发出多种方法，包括载体优化、培养基配方、培养参数和细胞工程。本综述简明扼要地概述了这些方法，同时也探讨了生产过程中遇到的挑战，如聚集和岩藻糖基化问题。
Enhancing recombinant antibody yield in Chinese hamster ovary cells
ABSTRACT
A range of recombinant monoclonal antibodies (rMAbs) have found application in treating diverse diseases, spanning various cancers and immune system disorders. Chinese hamster ovary (CHO) cells have emerged as the predominant choice for producing these rMAbs due to their robustness, ease of transfection, and capacity for posttranslational modifications akin to those in human cells. Transient transfection and/or stable expression could be conducted to express rMAbs in CHO cells. To bolster the yield of rMAbs in CHO cells, a multitude of approaches have been developed, encompassing vector optimization, medium formulation, cultivation parameters, and cell engineering. This review succinctly outlines these methodologies when also addressing challenges encountered in the production process, such as issues with aggregation and fucosylation.
期刊介绍：
The Tzu Chi Medical Journal is the peer-reviewed publication of the Buddhist Compassion Relief Tzu Chi Foundation, and includes original research papers on clinical medicine and basic science, case reports, clinical pathological pages, and review articles.
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