管水母(Holothuria tubulosa)的幼虫发育--一种新的可控进化系统

IF 2.4 3区 环境科学与生态学 Q2 ECOLOGY Frontiers in Ecology and Evolution Pub Date : 2024-05-24 DOI:10.3389/fevo.2024.1409174
M. Perillo, Tanya Alessandro, Alfonso Toscano, Rossella Annunziata
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引用次数: 0

摘要

要探索动物的多样性,必须建立新的可进行实验的生物体。一个多世纪以来,棘皮动物包括五类海洋动物,由于其成本低、繁殖力强、幼虫光学清晰和遗传可控性强,一直被用作发育模型。棘皮动物的另一个优势是其幼虫形态各异。这种丰富的多样性使比较研究能够探究细胞类型、组织和器官之间的进化关系。然而,获取配子的可重现方案、胚胎发生的详细信息以及基因组工具仅针对部分海胆和海星物种进行了优化。为了填补这一空白,我们建立了丰富的地中海海参 Holothuria tubulosa 作为新的实验系统。在这里,我们描述了一种方法,可以从同一动物身上多次可靠地获得配子和进行胚胎培养,并结合免疫组化和高分辨率显微镜鉴定独特的幼体组织。这项工作标志着我们对全毛蜥发育的认识又向前迈进了一步,并将管毛蜥确立为进化-反演和其他生物学科的新兴实验系统。
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Larval development of Holothuria tubulosa, a new tractable system for evo-devo
To explore animal diversity, new experimentally tractable organisms must be established. Echinoderms include five groups of marine animals that have been used as developmental models for over a century thanks to their low costs, high fecundity, optically clear larvae and genetic tractability. An additional advantage of echinoderms is that their larval forms display diverse morphologies. This rich diversity enables comparative studies to investigate the evolutionary relationships among cell types, tissues, and organs. However, reproducible protocols to obtain gametes, detailed information on embryogenesis, and genomic tools have been optimized only for selected species of sea urchins and sea stars. To address this gap, we established the abundant Mediterranean sea cucumber Holothuria tubulosa as a new experimental system. Here we describe a method to reliably obtain gametes and make embryonic cultures multiple times from the same animal and characterize unique larval tissues combining immunohistochemistry and high-resolution microscopy. This work represents a step forward in our understanding of holothurian development and establishes H. tubulosa as an emerging experimental system for evo-devo and other biological disciplines.
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来源期刊
Frontiers in Ecology and Evolution
Frontiers in Ecology and Evolution Environmental Science-Ecology
CiteScore
4.00
自引率
6.70%
发文量
1143
审稿时长
12 weeks
期刊介绍: Frontiers in Ecology and Evolution publishes rigorously peer-reviewed research across fundamental and applied sciences, to provide ecological and evolutionary insights into our natural and anthropogenic world, and how it should best be managed. Field Chief Editor Mark A. Elgar at the University of Melbourne is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics and the public worldwide. Eminent biologist and theist Theodosius Dobzhansky’s astute observation that “Nothing in biology makes sense except in the light of evolution” has arguably even broader relevance now than when it was first penned in The American Biology Teacher in 1973. One could similarly argue that not much in evolution makes sense without recourse to ecological concepts: understanding diversity — from microbial adaptations to species assemblages — requires insights from both ecological and evolutionary disciplines. Nowadays, technological developments from other fields allow us to address unprecedented ecological and evolutionary questions of astonishing detail, impressive breadth and compelling inference. The specialty sections of Frontiers in Ecology and Evolution will publish, under a single platform, contemporary, rigorous research, reviews, opinions, and commentaries that cover the spectrum of ecological and evolutionary inquiry, both fundamental and applied. Articles are peer-reviewed according to the Frontiers review guidelines, which evaluate manuscripts on objective editorial criteria. Through this unique, Frontiers platform for open-access publishing and research networking, Frontiers in Ecology and Evolution aims to provide colleagues and the broader community with ecological and evolutionary insights into our natural and anthropogenic world, and how it might best be managed.
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