臭氧对甲氨蝶呤引起的小鼠睾丸损伤的保护作用。

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-08-26 DOI:10.5935/1518-0557.20240041
Layasadat Khorsandi, Negar Varaa, Reza Dadfar, Sadegh Moradi Vastegani, Yousef Asadi-Fard, Akram Ahangarpour, Amirhesam Keshavarz-Zarjani
{"title":"臭氧对甲氨蝶呤引起的小鼠睾丸损伤的保护作用。","authors":"Layasadat Khorsandi, Negar Varaa, Reza Dadfar, Sadegh Moradi Vastegani, Yousef Asadi-Fard, Akram Ahangarpour, Amirhesam Keshavarz-Zarjani","doi":"10.5935/1518-0557.20240041","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Methotrexate (MTX) is widely administered for the treatment of various cancers. However, MTX induces male reproductive toxicity. In the current study, the effect of ozone therapy (OT) on reducing the toxic effects of MTX in the mouse testicles has been investigated.</p><p><strong>Methods: </strong>Twenty-four mice were divided into four groups: control, OT (4 mg/kg ozone), MTX (20 mg/kg), and MTX + OT. Testosterone levels, histological changes, and oxidative stress biomarkers were assessed to evaluate the protective effects of OT.</p><p><strong>Results: </strong>The results demonstrated that MTX disrupted germinal epithelium, reduced serum testosterone levels, and enhanced oxidative stress in testicular tissue. However, treatment with OT attenuated these adverse effects. OT effectively restored the levels of antioxidant enzymes, such as catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD). OT reduced lipid peroxidation, as indicated by decreased malondialdehyde (MDA) levels. OT preserved normal spermatogenesis, improved morphometric parameters, and reduced histological changes by MTX. Moreover, OT effectively restored testosterone levels.</p><p><strong>Conclusions: </strong>OT protects against MTX-induced testicular damage by suppressing oxidative stress.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"464-470"},"PeriodicalIF":1.8000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11349260/pdf/","citationCount":"0","resultStr":"{\"title\":\"The protective effect of Ozone on the mice testicular damage induced by methotrexate.\",\"authors\":\"Layasadat Khorsandi, Negar Varaa, Reza Dadfar, Sadegh Moradi Vastegani, Yousef Asadi-Fard, Akram Ahangarpour, Amirhesam Keshavarz-Zarjani\",\"doi\":\"10.5935/1518-0557.20240041\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Methotrexate (MTX) is widely administered for the treatment of various cancers. However, MTX induces male reproductive toxicity. In the current study, the effect of ozone therapy (OT) on reducing the toxic effects of MTX in the mouse testicles has been investigated.</p><p><strong>Methods: </strong>Twenty-four mice were divided into four groups: control, OT (4 mg/kg ozone), MTX (20 mg/kg), and MTX + OT. Testosterone levels, histological changes, and oxidative stress biomarkers were assessed to evaluate the protective effects of OT.</p><p><strong>Results: </strong>The results demonstrated that MTX disrupted germinal epithelium, reduced serum testosterone levels, and enhanced oxidative stress in testicular tissue. However, treatment with OT attenuated these adverse effects. OT effectively restored the levels of antioxidant enzymes, such as catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD). OT reduced lipid peroxidation, as indicated by decreased malondialdehyde (MDA) levels. OT preserved normal spermatogenesis, improved morphometric parameters, and reduced histological changes by MTX. Moreover, OT effectively restored testosterone levels.</p><p><strong>Conclusions: </strong>OT protects against MTX-induced testicular damage by suppressing oxidative stress.</p>\",\"PeriodicalId\":46364,\"journal\":{\"name\":\"Jornal Brasileiro de Reproducao Assistida\",\"volume\":\" \",\"pages\":\"464-470\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11349260/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jornal Brasileiro de Reproducao Assistida\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5935/1518-0557.20240041\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jornal Brasileiro de Reproducao Assistida","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5935/1518-0557.20240041","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

目的:甲氨蝶呤(MTX)被广泛用于治疗各种癌症。然而,MTX 会诱发雄性生殖毒性。本研究探讨了臭氧疗法(OT)对减轻 MTX 在小鼠睾丸中毒性作用的影响:24只小鼠分为四组:对照组、OT组(4毫克/千克臭氧)、MTX组(20毫克/千克)和MTX + OT组。评估睾酮水平、组织学变化和氧化应激生物标志物,以评价 OT 的保护作用:结果表明:MTX破坏了生殖上皮,降低了血清睾酮水平,并增强了睾丸组织的氧化应激。然而,用 OT 治疗可减轻这些不良影响。OT 能有效恢复抗氧化酶的水平,如过氧化氢酶(CAT)、谷胱甘肽(GSH)和超氧化物歧化酶(SOD)。OT降低了脂质过氧化反应,表现为丙二醛(MDA)水平的下降。OT 可保持正常的精子发生,改善形态计量参数,并减少 MTX 引起的组织学变化。此外,OT 还能有效恢复睾酮水平:结论:OT可通过抑制氧化应激防止MTX诱发的睾丸损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The protective effect of Ozone on the mice testicular damage induced by methotrexate.

Objective: Methotrexate (MTX) is widely administered for the treatment of various cancers. However, MTX induces male reproductive toxicity. In the current study, the effect of ozone therapy (OT) on reducing the toxic effects of MTX in the mouse testicles has been investigated.

Methods: Twenty-four mice were divided into four groups: control, OT (4 mg/kg ozone), MTX (20 mg/kg), and MTX + OT. Testosterone levels, histological changes, and oxidative stress biomarkers were assessed to evaluate the protective effects of OT.

Results: The results demonstrated that MTX disrupted germinal epithelium, reduced serum testosterone levels, and enhanced oxidative stress in testicular tissue. However, treatment with OT attenuated these adverse effects. OT effectively restored the levels of antioxidant enzymes, such as catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD). OT reduced lipid peroxidation, as indicated by decreased malondialdehyde (MDA) levels. OT preserved normal spermatogenesis, improved morphometric parameters, and reduced histological changes by MTX. Moreover, OT effectively restored testosterone levels.

Conclusions: OT protects against MTX-induced testicular damage by suppressing oxidative stress.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.30
自引率
6.70%
发文量
56
期刊最新文献
Effects of dietary quercetin on retrieved mouse oocytes and in vitro fertilization outcomes. Increased antinuclear (anti-Sm) antibodies in infertile patients with peritoneal endometriosis. Microfluidic in compared with Zeta potential, MACS and swim up methods, resulted in improved chromatin integrity and high quality sperms. Quality of information on fertility clinic websites accredited by the Latin American Network of Assisted Reproduction. Deleterious Effects of Caffeine Consumption on Reproductive Functions of Female Wistar Rats.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1