{"title":"鲍曼不动杆菌临床分离株中 1 类整合子、碳青霉烯酶基因和生物膜形成基因的发生率分析。","authors":"Yu Xiu, Yueru Dai, Shasha Yin, Quhao Wei","doi":"10.33073/pjm-2024-017","DOIUrl":null,"url":null,"abstract":"<p><p><i>Acinetobacter baumannii</i> is a non-fermentative Gram-negative bacterium that can cause nosocomial infections in critically ill patients. Carbapenem-resistant <i>A. baumannii</i> (CRAB) has spread rapidly in clinical settings and has become a key concern. The main objective of this study was to identify the distribution of integrons and biofilm-formation-related virulence genes in CRAB isolates. A total of 269 <i>A. baumannii</i> isolates (219 isolates of CRAB and 50 isolates of carbapenem-sensitive <i>A. baumannii</i> (CSAB)) were collected. Carbapenemase genes (<i>bla</i> <sub>KPC</sub>, <i>bla</i> <sub>VIM</sub>, <i>bla</i> <sub>IMP</sub>, <i>bla</i> <sub>NDM</sub>, and <i>bla</i> <sub>OXA-23-like</sub>) and biofilm-formation-related virulence genes (<i>abal</i>, <i>bfms</i>, <i>bap</i>, and <i>cusE</i>) were screened with PCR. Class 1 integron was screened with PCR, and common promoters and gene cassette arrays were determined with restriction pattern analysis combined with primer walking sequencing. Whole-genome sequencing was conducted, and data were analyzed for a <i>bla</i> <sub>OXA-23-like</sub>-negative isolate. All 219 CRAB isolates were negative for <i>bla</i> <sub>KPC</sub>, <i>bla</i> <sub>VIM</sub>, <i>bla</i> <sub>IMP</sub>, and <i>bla</i> <sub>NDM</sub>, while <i>bla</i> <sub>OXA-23-like</sub> was detected in 218 isolates. The detection rates for <i>abal</i>, <i>bfms</i>, <i>bap</i>, and <i>cusE</i> in 219 CRAB were 93.15%, 63.93%, 88.13%, and 77.63%, respectively. Class 1 integron was detected in 75 CRAB (34.25%) and in 3 CSAB. The single gene cassette array <i>aacA4-catB8-aadA1</i> with relatively strong PcH2 promoter was detected in class 1 integrons. The <i>bla</i> <sub>OXA-23-like</sub>-negative CRAB isolate was revealed to be a new sequence type (Oxford 3272, Pasteur 2520) carrying <i>bla</i> <sub>OXA-72</sub>, <i>bla</i> <sub>OXA-259</sub>, and <i>bla</i> <sub>ADC-26</sub>. In conclusion, <i>bla</i> <sub>OXA-23-like</sub> was the main reason for CRAB's resistance to carbapenems. A new (Oxford 3272, Pasteur 2520) CRAB sequence type carrying the <i>bla</i> <sub>OXA-72</sub>, <i>bla</i> <sub>OXA-259</sub>, and <i>bla</i> <sub>ADC-26</sub> was reported.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":" ","pages":"189-197"},"PeriodicalIF":0.0000,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192457/pdf/","citationCount":"0","resultStr":"{\"title\":\"Analysis of the Class 1 Integrons, Carbapenemase Genes and Biofilm Formation Genes Occurrence in <i>Acinetobacter baumannii</i> Clinical Isolates.\",\"authors\":\"Yu Xiu, Yueru Dai, Shasha Yin, Quhao Wei\",\"doi\":\"10.33073/pjm-2024-017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Acinetobacter baumannii</i> is a non-fermentative Gram-negative bacterium that can cause nosocomial infections in critically ill patients. Carbapenem-resistant <i>A. baumannii</i> (CRAB) has spread rapidly in clinical settings and has become a key concern. The main objective of this study was to identify the distribution of integrons and biofilm-formation-related virulence genes in CRAB isolates. A total of 269 <i>A. baumannii</i> isolates (219 isolates of CRAB and 50 isolates of carbapenem-sensitive <i>A. baumannii</i> (CSAB)) were collected. Carbapenemase genes (<i>bla</i> <sub>KPC</sub>, <i>bla</i> <sub>VIM</sub>, <i>bla</i> <sub>IMP</sub>, <i>bla</i> <sub>NDM</sub>, and <i>bla</i> <sub>OXA-23-like</sub>) and biofilm-formation-related virulence genes (<i>abal</i>, <i>bfms</i>, <i>bap</i>, and <i>cusE</i>) were screened with PCR. Class 1 integron was screened with PCR, and common promoters and gene cassette arrays were determined with restriction pattern analysis combined with primer walking sequencing. Whole-genome sequencing was conducted, and data were analyzed for a <i>bla</i> <sub>OXA-23-like</sub>-negative isolate. All 219 CRAB isolates were negative for <i>bla</i> <sub>KPC</sub>, <i>bla</i> <sub>VIM</sub>, <i>bla</i> <sub>IMP</sub>, and <i>bla</i> <sub>NDM</sub>, while <i>bla</i> <sub>OXA-23-like</sub> was detected in 218 isolates. The detection rates for <i>abal</i>, <i>bfms</i>, <i>bap</i>, and <i>cusE</i> in 219 CRAB were 93.15%, 63.93%, 88.13%, and 77.63%, respectively. Class 1 integron was detected in 75 CRAB (34.25%) and in 3 CSAB. The single gene cassette array <i>aacA4-catB8-aadA1</i> with relatively strong PcH2 promoter was detected in class 1 integrons. The <i>bla</i> <sub>OXA-23-like</sub>-negative CRAB isolate was revealed to be a new sequence type (Oxford 3272, Pasteur 2520) carrying <i>bla</i> <sub>OXA-72</sub>, <i>bla</i> <sub>OXA-259</sub>, and <i>bla</i> <sub>ADC-26</sub>. In conclusion, <i>bla</i> <sub>OXA-23-like</sub> was the main reason for CRAB's resistance to carbapenems. A new (Oxford 3272, Pasteur 2520) CRAB sequence type carrying the <i>bla</i> <sub>OXA-72</sub>, <i>bla</i> <sub>OXA-259</sub>, and <i>bla</i> <sub>ADC-26</sub> was reported.</p>\",\"PeriodicalId\":94173,\"journal\":{\"name\":\"Polish journal of microbiology\",\"volume\":\" \",\"pages\":\"189-197\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192457/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polish journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33073/pjm-2024-017\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/6/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33073/pjm-2024-017","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
鲍曼不动杆菌(Acinetobacter baumannii)是一种非发酵革兰氏阴性细菌,可引起重症患者的院内感染。耐碳青霉烯鲍曼不动杆菌(CRAB)已在临床环境中迅速传播,并已成为人们关注的焦点。本研究的主要目的是鉴定 CRAB 分离物中整合子和生物膜形成相关毒力基因的分布。本研究共收集了269株鲍曼不动杆菌分离株(219株CRAB分离株和50株碳青霉烯类敏感鲍曼不动杆菌(CSAB)分离株)。用 PCR 筛选了碳青霉烯酶基因(bla KPC、bla VIM、bla IMP、bla NDM 和 bla OXA-23-like)和与生物膜形成相关的毒力基因(abal、bfms、bap 和 cusE)。用 PCR 筛选了 1 类整合子,并通过限制性模式分析和引物走行测序确定了常见启动子和基因盒阵列。进行了全基因组测序,并对 bla OXA-23 样阴性分离物进行了数据分析。所有 219 个 CRAB 分离物的 bla KPC、bla VIM、bla IMP 和 bla NDM 均为阴性,而 218 个分离物中检测到了 bla OXA-23-like。在 219 个 CRAB 分离物中,abal、bfms、bap 和 cusE 的检出率分别为 93.15%、63.93%、88.13% 和 77.63%。在 75 个 CRAB(34.25%)和 3 个 CSAB 中检测到了 1 类整合子。在 1 类整合子中检测到了带有相对较强的 PcH2 启动子的单基因盒阵列 aacA4-catB8-aadA1。bla OXA-23 样阴性的 CRAB 分离物被发现是一种新的序列类型(Oxford 3272,Pasteur 2520),携带 bla OXA-72、bla OXA-259 和 bla ADC-26。总之,类 bla OXA-23 是 CRAB 对碳青霉烯类产生耐药性的主要原因。报告了一种携带 bla OXA-72、bla OXA-259 和 bla ADC-26 的新 CRAB 序列类型(牛津 3272,巴斯德 2520)。
Analysis of the Class 1 Integrons, Carbapenemase Genes and Biofilm Formation Genes Occurrence in Acinetobacter baumannii Clinical Isolates.
Acinetobacter baumannii is a non-fermentative Gram-negative bacterium that can cause nosocomial infections in critically ill patients. Carbapenem-resistant A. baumannii (CRAB) has spread rapidly in clinical settings and has become a key concern. The main objective of this study was to identify the distribution of integrons and biofilm-formation-related virulence genes in CRAB isolates. A total of 269 A. baumannii isolates (219 isolates of CRAB and 50 isolates of carbapenem-sensitive A. baumannii (CSAB)) were collected. Carbapenemase genes (blaKPC, blaVIM, blaIMP, blaNDM, and blaOXA-23-like) and biofilm-formation-related virulence genes (abal, bfms, bap, and cusE) were screened with PCR. Class 1 integron was screened with PCR, and common promoters and gene cassette arrays were determined with restriction pattern analysis combined with primer walking sequencing. Whole-genome sequencing was conducted, and data were analyzed for a blaOXA-23-like-negative isolate. All 219 CRAB isolates were negative for blaKPC, blaVIM, blaIMP, and blaNDM, while blaOXA-23-like was detected in 218 isolates. The detection rates for abal, bfms, bap, and cusE in 219 CRAB were 93.15%, 63.93%, 88.13%, and 77.63%, respectively. Class 1 integron was detected in 75 CRAB (34.25%) and in 3 CSAB. The single gene cassette array aacA4-catB8-aadA1 with relatively strong PcH2 promoter was detected in class 1 integrons. The blaOXA-23-like-negative CRAB isolate was revealed to be a new sequence type (Oxford 3272, Pasteur 2520) carrying blaOXA-72, blaOXA-259, and blaADC-26. In conclusion, blaOXA-23-like was the main reason for CRAB's resistance to carbapenems. A new (Oxford 3272, Pasteur 2520) CRAB sequence type carrying the blaOXA-72, blaOXA-259, and blaADC-26 was reported.