pH 值选择热带土壤微生态系统中不同的氧化亚氮还原微生物群。

IF 5.1 Q1 ECOLOGY ISME communications Pub Date : 2024-05-08 eCollection Date: 2024-01-01 DOI:10.1093/ismeco/ycae070
Yanchen Sun, Yongchao Yin, Guang He, Gyuhyon Cha, Héctor L Ayala-Del-Río, Grizelle González, Konstantinos T Konstantinidis, Frank E Löffler
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引用次数: 0

摘要

一氧化二氮(N2O)是一种具有破坏臭氧潜能的温室气体,可通过微生物在一氧化二氮还原酶(NosZ)催化下还原成二氮而得到缓解。对具有 NosZ 活性的细菌进行了中性 pH 值的研究,但对低 pH 值 N2O 还原的微生物学研究一直没有结果。酸性(pH 2O)评估了 pH 值为 4.5 和 7.3 时的 N2O 还原情况。所有微生态系统都消耗 N2O,在 2 mM N2O 的微生态系统中观察到的滞后时间长达 7 个月。元基因组比较分析表明,在两种 N2O 摄食机制下,Rhodocyclaceae 在环中性微生态系统中都占主导地位。在 pH 值为 4.5 时,Peptococcaceae 在高 N2O 微生态中占优势,而 Hyphomicrobiaceae 在低 N2O 微生态中占优势。从还原 N2O 的微生态环境中回收的 17 个高质量元基因组(MAGs)含有 nos 操作子,其中来自酸性微生态环境的 8 个 MAGs 都携带第二支系类型的 nosZ,并且缺乏亚硝酸盐还原酶基因(nirS/K)。在 pH 值为 4.5 的微生态系统中发现的 8 个 MAGs 中,有 5 个是新的类群,这表明热带酸性土壤中存在尚未开发的 N2O 还原多样性。对 pH 值为 3.5-5.7 的土壤元基因组数据集的调查显示,nosZ 基因普遍存在,这表明酸性土壤中的 N2O 还原潜力分布广泛。
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pH selects for distinct N2O-reducing microbiomes in tropical soil microcosms.

Nitrous oxide (N2O), a greenhouse gas with ozone destruction potential, is mitigated by the microbial reduction to dinitrogen catalyzed by N2O reductase (NosZ). Bacteria with NosZ activity have been studied at circumneutral pH but the microbiology of low pH N2O reduction has remained elusive. Acidic (pH < 5) tropical forest soils were collected in the Luquillo Experimental Forest in Puerto Rico, and microcosms maintained with low (0.02 mM) and high (2 mM) N2O assessed N2O reduction at pH 4.5 and 7.3. All microcosms consumed N2O, with lag times of up to 7 months observed in microcosms with 2 mM N2O. Comparative metagenome analysis revealed that Rhodocyclaceae dominated in circumneutral microcosms under both N2O feeding regimes. At pH 4.5, Peptococcaceae dominated in high-N2O, and Hyphomicrobiaceae in low-N2O microcosms. Seventeen high-quality metagenome-assembled genomes (MAGs) recovered from the N2O-reducing microcosms harbored nos operons, with all eight MAGs derived from acidic microcosms carrying the Clade II type nosZ and lacking nitrite reductase genes (nirS/K). Five of the eight MAGs recovered from pH 4.5 microcosms represent novel taxa indicating an unexplored N2O-reducing diversity exists in acidic tropical soils. A survey of pH 3.5-5.7 soil metagenome datasets revealed that nosZ genes commonly occur, suggesting broad distribution of N2O reduction potential in acidic soils.

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