基于网络药理学探索依西那丁抗宫颈癌的机制

IF 2.1 4区 医学 Q3 PHARMACOLOGY & PHARMACY Journal of Clinical Pharmacy and Therapeutics Pub Date : 2024-04-08 DOI:10.1155/2024/7008847
Hu Chen, Jinlei Wang
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引用次数: 0

摘要

宫颈癌死亡率高,在年轻人中的发病率越来越高,严重威胁着妇女的健康。在传统中药中,棘白是甘草的主要活性成分。然而,将其用于宫颈癌治疗的研究却很有限。在我们的研究中,一些数据库确定了 198 个棘白的靶点。在这些靶点中,我们选择了 40 个与宫颈癌相关的核心靶点。富集分析显示,Echinatin通过与细胞周期、细胞凋亡、衰老和各种癌症相关信号通路有关的基因发挥作用。交叉靶点的差异表达已在 GEO 数据库中得到验证,分子对接也表明活性化合物与确定的靶点之间有很强的结合能力。此外,Western 印迹的结果在蛋白质水平上提供了进一步的证据。棘白抑制了HeLa和SiHa细胞的增殖、迁移和侵袭,同时增加了它们的凋亡。这项研究预测了刺五加在宫颈癌治疗中的潜在靶点和有益作用,为进一步研究刺五加在宫颈癌治疗中的分子机制提供了新的途径。
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Exploring the Mechanism of Echinatin against Cervical Cancer Based on Network Pharmacology

Cervical cancer significantly impacts women’s health due to its high mortality rate and increasing prevalence among younger individuals, thereby posing a severe threat. Echinatin is the primary active component of licorice in traditional Chinese medicine. However, studies on its use in cervical cancer treatment are limited. In our study, 198 targets of Echinatin were identified by some databases. Among these, 40 core targets related to cervical cancer were selected. Enrichment analyses revealed that Echinatin operated through genes associated with cell cycle, apoptosis, senescence, and various cancer-related signaling pathways. Differential expression of intersecting targets was verified in the GEO database, and molecular docking also indicated a strong binding capacity between active compounds and identified targets. Moreover, the results of western blot provided further evidence at the protein level. Echinatin hindered the proliferation, migration, and invasion of HeLa and SiHa cells while increasing their apoptosis. This study predicted the potential targets and beneficial effects of Echinatin in cervical cancer treatment, which provides a new avenue for further research into the molecular mechanisms underlying the role of Echinatin in cervical cancer treatment.

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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
226
审稿时长
6 months
期刊介绍: The Journal of Clinical Pharmacy and Therapeutics provides a forum for clinicians, pharmacists and pharmacologists to explore and report on issues of common interest. Reports and commentaries on current issues in medical and pharmaceutical practice are encouraged. Papers on evidence-based clinical practice and multidisciplinary collaborative work are particularly welcome. Regular sections in the journal include: editorials, commentaries, reviews (including systematic overviews and meta-analyses), original research and reports, and book reviews. Its scope embraces all aspects of clinical drug development and therapeutics, including: Rational therapeutics Evidence-based practice Safety, cost-effectiveness and clinical efficacy of drugs Drug interactions Clinical impact of drug formulations Pharmacogenetics Personalised, stratified and translational medicine Clinical pharmacokinetics.
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