Franco Daniel Fernández, Fabiana Aída Guzmán, Luis Rogelio Conci
{"title":"Cicuta 女巫扫帚植物支原体 16SrIII-J 亚群基因组序列草案：南美洲植物病理学相关亚群","authors":"Franco Daniel Fernández, Fabiana Aída Guzmán, Luis Rogelio Conci","doi":"10.1007/s40858-024-00658-0","DOIUrl":null,"url":null,"abstract":"<p>Phytoplasmas are uncultivable wall-less bacteria causing plant diseases worldwide. Phytoplasmas from X-disease are one of the most diverse and economically important in South America. The Cicuta witches' broom Phytoplasma (CicWB) was described in association to cicuta or hemlock (<i>Conium maculatum</i> L.) exhibiting witches' broom and phyllody symptoms in surrounding potato fields in Los Cerrillos (Córdoba- Argentina). The partial genome of CicWB phytoplasma was obtained using a hybrid approach combining Illumina and Oxford Nanopore Technologies. Final draft genome consists of 16 contigs totaling 758.187 bp of length, with 400X of coverage and 96.70% of the estimated completeness. Comparative genomics, based on Average Nucleotide Identity (ANI), digital DNA-DNA hybridization (dDDH), and phylogenomic analyses, revealed its close relationship to subgroup 16SrIII-J phytoplasmas. Furthermore, the study identified 37 putative effectors, including orthologs for SAP11, SAP54, SAP05, as well as immunodominant membrane proteins Imp and IdpA<i>.</i> This comprehensive genome analysis provides crucial insights into the genomic landscape of phytoplasmas in the region, contributing to our understanding of their diversity and pathogenicity.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Draft genome sequence of Cicuta witches' broom phytoplasma, subgroup 16SrIII-J: a subgroup with phytopathological relevance in South America\",\"authors\":\"Franco Daniel Fernández, Fabiana Aída Guzmán, Luis Rogelio Conci\",\"doi\":\"10.1007/s40858-024-00658-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Phytoplasmas are uncultivable wall-less bacteria causing plant diseases worldwide. Phytoplasmas from X-disease are one of the most diverse and economically important in South America. The Cicuta witches' broom Phytoplasma (CicWB) was described in association to cicuta or hemlock (<i>Conium maculatum</i> L.) exhibiting witches' broom and phyllody symptoms in surrounding potato fields in Los Cerrillos (Córdoba- Argentina). The partial genome of CicWB phytoplasma was obtained using a hybrid approach combining Illumina and Oxford Nanopore Technologies. Final draft genome consists of 16 contigs totaling 758.187 bp of length, with 400X of coverage and 96.70% of the estimated completeness. Comparative genomics, based on Average Nucleotide Identity (ANI), digital DNA-DNA hybridization (dDDH), and phylogenomic analyses, revealed its close relationship to subgroup 16SrIII-J phytoplasmas. Furthermore, the study identified 37 putative effectors, including orthologs for SAP11, SAP54, SAP05, as well as immunodominant membrane proteins Imp and IdpA<i>.</i> This comprehensive genome analysis provides crucial insights into the genomic landscape of phytoplasmas in the region, contributing to our understanding of their diversity and pathogenicity.</p>\",\"PeriodicalId\":23354,\"journal\":{\"name\":\"Tropical Plant Pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s40858-024-00658-0\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00658-0","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Draft genome sequence of Cicuta witches' broom phytoplasma, subgroup 16SrIII-J: a subgroup with phytopathological relevance in South America
Phytoplasmas are uncultivable wall-less bacteria causing plant diseases worldwide. Phytoplasmas from X-disease are one of the most diverse and economically important in South America. The Cicuta witches' broom Phytoplasma (CicWB) was described in association to cicuta or hemlock (Conium maculatum L.) exhibiting witches' broom and phyllody symptoms in surrounding potato fields in Los Cerrillos (Córdoba- Argentina). The partial genome of CicWB phytoplasma was obtained using a hybrid approach combining Illumina and Oxford Nanopore Technologies. Final draft genome consists of 16 contigs totaling 758.187 bp of length, with 400X of coverage and 96.70% of the estimated completeness. Comparative genomics, based on Average Nucleotide Identity (ANI), digital DNA-DNA hybridization (dDDH), and phylogenomic analyses, revealed its close relationship to subgroup 16SrIII-J phytoplasmas. Furthermore, the study identified 37 putative effectors, including orthologs for SAP11, SAP54, SAP05, as well as immunodominant membrane proteins Imp and IdpA. This comprehensive genome analysis provides crucial insights into the genomic landscape of phytoplasmas in the region, contributing to our understanding of their diversity and pathogenicity.
期刊介绍：
Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.
Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection.
The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines.
Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
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