Caspase-3活性和自噬强度在神经元抵御谷氨酸盐毒性过程中的作用

IF 0.5 4区医学 Q4 NEUROSCIENCES Neurochemical Journal Pub Date : 2024-05-27 DOI:10.1134/s1819712424020028

O. P. Aleksandrova, D. V. Kuznetsova, A. A. Lyzhin, L. G. Khaspekov, N. V. Gulyaeva, A. A. Yakovlev

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引用次数: 0

摘要

摘要在神经元对有毒谷氨酸挑战（营养因子剥夺）的抗性模型中，自噬激活的两个时期对抗性的发展具有不同的意义。浓度为 1.25 毫摩尔的自噬抑制剂 3-甲基腺嘌呤（3-MA）能显著抑制抗性的发展，但只有在营养因子被剥夺后立即使用才能抑制抗性的发展。在剥夺营养因子期间用 3-MA 抑制自噬不会影响抗性的产生。此外，自噬的激活会导致 Caspase-3 活性下降，但这一过程的机制仍不清楚。我们推测，神经元抵抗力的产生是由自噬激活导致的 Caspase-3 活性下降介导的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Caspase-3 Activity and Autophagy Intensity in the Development of Neuronal Resistance to Glutamate Toxicity

Abstract

Two periods of autophagy activation with a different significance for the development of resistance were demonstrated in the model of neuronal resistance to the toxic glutamate challenge (trophic factor deprivation). The autophagy inhibitor 3-methyladenine (3-MA) at a concentration of 1.25 mM significantly suppressed resistance development but only if applied immediately after deprivation of trophic factors. Inhibition of autophagy with 3-MA during deprivation did not affect resistance production. In addition, activation of autophagy caused a decrease in caspase-3 activity, although the mechanism of this process remains unclear. We hypothesize that development of resistance in neurons is mediated by a decrease in caspase-3 activity caused by autophagy activation.

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来源期刊

Neurochemical Journal 医学-神经科学

自引率

20.00%

发文量

审稿时长

>12 weeks

期刊介绍： Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components. The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.