Yanlei Kong, Feilong Zhang, Nan Zhou, Hongyu Zhang, Hao Wang
{"title":"二氧化硅-羟基磷灰石共轭高氧化铈纳米粒子对正畸托槽的抗菌和细胞活力效应的演变","authors":"Yanlei Kong, Feilong Zhang, Nan Zhou, Hongyu Zhang, Hao Wang","doi":"10.1007/s10924-024-03325-2","DOIUrl":null,"url":null,"abstract":"<div><p>Nanomaterials have been utilized as antibacterial agents due to their distinct mechanism of action, which sets them apart from traditional antibiotics. Cerium oxide nanoparticles (CeO2 NPs) play a crucial role as antibacterial agents because of their minimal toxicity towards healthy cells and their unique antibacterial mechanism, which involves the reversible transition between two valence states of Ce(III)/Ce(IV). In the current study, silica microspheres (SiO<sub>2</sub>) were applied for the growth of hydroxyapatite (Hap) over the silica surface. Then, the SiO<sub>2</sub>/hydroxyapatite (SiO<sub>2</sub>/Hap) nanocomposite was applied for stabilization of the in <i>situ</i> green synthesized cerium oxide nanoparticles (CeO<sub>2</sub> NPs) on its surface to assess the antibacterial and cellular viability of orthodontic brackets. The as-prepared SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs were fully characterized by different techniques such as FT-IR, XRD, FE-SEM, TEM and ICP-OES analyses. The technique employed to coat the orthodontic bands with SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite was the electrostatic spray-assisted vapor deposition method. The antibacterial efficacy of SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite against <i>Candida albicans</i>, <i>Streptococcus mutans</i> and <i>Lactobacillus acidophilus</i> was evaluated using the biofilm inhibition test. In the recent study’s cellular and molecular component, the treated cells with SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite underwent evaluation through MTT assay to determine cytotoxicity on the normal (HUVEC) cell line. In comparison to the control group, the SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite group exhibited a 2-log10 decrease in the replication ability of all microorganisms. The group of SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite demonstrated a notable decrease in the quantity of <i>C. albicans</i>, <i>S. mutans</i> and <i>L. acidophilus</i> colonies compared to the control group. Application of SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite on orthodontic bands resulted in antibacterial properties against oral pathogens.</p></div>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evolution of the Antibacterial and Cellular Viability Effects of Silica-Hydroxyapatite Conjugated High Cerium Oxide Nanoparticles on Orthodontic Brackets\",\"authors\":\"Yanlei Kong, Feilong Zhang, Nan Zhou, Hongyu Zhang, Hao Wang\",\"doi\":\"10.1007/s10924-024-03325-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Nanomaterials have been utilized as antibacterial agents due to their distinct mechanism of action, which sets them apart from traditional antibiotics. Cerium oxide nanoparticles (CeO2 NPs) play a crucial role as antibacterial agents because of their minimal toxicity towards healthy cells and their unique antibacterial mechanism, which involves the reversible transition between two valence states of Ce(III)/Ce(IV). In the current study, silica microspheres (SiO<sub>2</sub>) were applied for the growth of hydroxyapatite (Hap) over the silica surface. Then, the SiO<sub>2</sub>/hydroxyapatite (SiO<sub>2</sub>/Hap) nanocomposite was applied for stabilization of the in <i>situ</i> green synthesized cerium oxide nanoparticles (CeO<sub>2</sub> NPs) on its surface to assess the antibacterial and cellular viability of orthodontic brackets. The as-prepared SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs were fully characterized by different techniques such as FT-IR, XRD, FE-SEM, TEM and ICP-OES analyses. The technique employed to coat the orthodontic bands with SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite was the electrostatic spray-assisted vapor deposition method. The antibacterial efficacy of SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite against <i>Candida albicans</i>, <i>Streptococcus mutans</i> and <i>Lactobacillus acidophilus</i> was evaluated using the biofilm inhibition test. In the recent study’s cellular and molecular component, the treated cells with SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite underwent evaluation through MTT assay to determine cytotoxicity on the normal (HUVEC) cell line. In comparison to the control group, the SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite group exhibited a 2-log10 decrease in the replication ability of all microorganisms. The group of SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite demonstrated a notable decrease in the quantity of <i>C. albicans</i>, <i>S. mutans</i> and <i>L. acidophilus</i> colonies compared to the control group. Application of SiO<sub>2</sub>/Hap-CeO<sub>2</sub> NPs nanocomposite on orthodontic bands resulted in antibacterial properties against oral pathogens.</p></div>\",\"PeriodicalId\":659,\"journal\":{\"name\":\"Journal of Polymers and the Environment\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Polymers and the Environment\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10924-024-03325-2\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10924-024-03325-2","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Evolution of the Antibacterial and Cellular Viability Effects of Silica-Hydroxyapatite Conjugated High Cerium Oxide Nanoparticles on Orthodontic Brackets
Nanomaterials have been utilized as antibacterial agents due to their distinct mechanism of action, which sets them apart from traditional antibiotics. Cerium oxide nanoparticles (CeO2 NPs) play a crucial role as antibacterial agents because of their minimal toxicity towards healthy cells and their unique antibacterial mechanism, which involves the reversible transition between two valence states of Ce(III)/Ce(IV). In the current study, silica microspheres (SiO2) were applied for the growth of hydroxyapatite (Hap) over the silica surface. Then, the SiO2/hydroxyapatite (SiO2/Hap) nanocomposite was applied for stabilization of the in situ green synthesized cerium oxide nanoparticles (CeO2 NPs) on its surface to assess the antibacterial and cellular viability of orthodontic brackets. The as-prepared SiO2/Hap-CeO2 NPs were fully characterized by different techniques such as FT-IR, XRD, FE-SEM, TEM and ICP-OES analyses. The technique employed to coat the orthodontic bands with SiO2/Hap-CeO2 NPs nanocomposite was the electrostatic spray-assisted vapor deposition method. The antibacterial efficacy of SiO2/Hap-CeO2 NPs nanocomposite against Candida albicans, Streptococcus mutans and Lactobacillus acidophilus was evaluated using the biofilm inhibition test. In the recent study’s cellular and molecular component, the treated cells with SiO2/Hap-CeO2 NPs nanocomposite underwent evaluation through MTT assay to determine cytotoxicity on the normal (HUVEC) cell line. In comparison to the control group, the SiO2/Hap-CeO2 NPs nanocomposite group exhibited a 2-log10 decrease in the replication ability of all microorganisms. The group of SiO2/Hap-CeO2 NPs nanocomposite demonstrated a notable decrease in the quantity of C. albicans, S. mutans and L. acidophilus colonies compared to the control group. Application of SiO2/Hap-CeO2 NPs nanocomposite on orthodontic bands resulted in antibacterial properties against oral pathogens.
期刊介绍：
The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
