丹参注射液通过HIF-1α/CXCR4/NF-κB信号通路抑制神经炎症,从而减轻脑缺血再灌注损伤。

IF 1.6 4区 医学 Q4 NEUROSCIENCES Neuroreport Pub Date : 2024-07-01 Epub Date: 2024-05-20 DOI:10.1097/WNR.0000000000002043
Gao Chen, Zhan Jin, Xi Wang, Qi-Hui Yu, Gao-Bo Hu
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引用次数: 0

摘要

丹参注射液对包括缺血性中风(IS)在内的心脑血管疾病有很好的治疗效果,但其作用机制尚不清楚。研究采用大脑中动脉闭塞模型模拟 SD 大鼠的缺血再灌注损伤。通过AAV-HIF-1α实现了缺氧诱导因子1α(HIF-1α)的过表达。大鼠接受 DI 或生理盐水治疗。评估神经系统评分和梗死率。通过 HE、2,3,5-三苯基四氮唑和 Nissl 染色检查 I/R 损伤。测量了相关蛋白质[TNF-α、IL-6、IL-1β、SOD、MDA、ROS、HIF-1α、CXC趋化因子受体4(CXCR4)和NF-κB]的表达水平。DI 治疗改善了神经系统评分,降低了梗死率,这表明它抑制了炎症和氧化应激。HIF-1α、CXCR4和NF-κB的表达水平有所下降。然而,HIF-1α过表达后,DI抑制炎症的效果就消失了。DI可能直接靶向HIF-1α,通过抑制HIF-1α/CXCR4/NF-κB信号通路来抑制神经炎症和减轻I/R损伤。
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Danshen injection mitigated the cerebral ischemia/reperfusion injury by suppressing neuroinflammation via the HIF-1α/CXCR4/NF-κB signaling pathway.

Danshen injection (DI) is effective in treating cardiovascular and cerebrovascular diseases, including ischemic stroke (IS), including IS, but its mechanism is unclear. A middle cerebral artery occlusion model was used to simulate ischemia/reperfusion (I/R) injury in SD rats. Overexpression of hypoxia-inducible factor 1α (HIF-1α) was achieved by AAV-HIF-1α. Rats were treated with DI or saline. Neurological scores and infarction rates were assessed. I/R damage was examined by HE, 2,3,5-triphenyltetrazolium and Nissl stainings. Expression levels of relative proteins [TNF-α, IL-6, IL-1β, SOD, MDA, ROS, HIF-1α, CXC chemokine receptor 4 (CXCR4) and NF-κB] were measured. DI treatment improved neurological scores and reduced infarction rates, suggesting that it inhibits inflammation and oxidative stress. The expression levels of HIF-1α, CXCR4 and NF-κB were decreased. However, the effectiveness of DI on inflammation inhibition was lost after HIF-1α overexpression. DI may directly target HIF-1α to suppress neuroinflammation and reduce I/R injury by suppressing the HIF-1α/CXCR4/NF-κB signaling pathway.

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来源期刊
Neuroreport
Neuroreport 医学-神经科学
CiteScore
3.20
自引率
0.00%
发文量
150
审稿时长
1 months
期刊介绍: NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.
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