酵母(Saccharomyces cerevisiae)生长细胞中的环AMP磷酸二酯酶活性。

K Suoranta
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引用次数: 0

摘要

测定了面包酵母(Saccharomyces cerevisiae)在2%葡萄糖条件下双补批生长过程中两种环AMP磷酸二酯酶的活性。在108 h的培养过程中,与mg无关的高Km磷酸二酯酶的比活性(单位/mg酵母蛋白)提高了20倍。mg依赖性、低Km磷酸二酯酶的比活性在葡萄糖利用过程中增加了一倍左右,并在细胞进入固定期时回落到初始水平。
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Cyclic AMP phosphodiesterase activities in growing cells of baker's yeast (Saccharomyces cerevisiae).

The activities of two cyclic AMP phosphodiesterases of baker's yeast (Saccharomyces cerevisiae) were measured during diauxic batch growth on 2% glucose. The specific activity (units/mg of yeast protein) of the Mg-independent, high Km phosphodiesterase increased 20-fold throughout the 108 h cultivation. The specific activity of the Mg-dependent, low Km phosphodiesterase about doubled during glucose utilization and fell back to the initial level as the cells entered stationary phase.

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