{"title":"藏身山中:南非发现两种新型山栖淡水蟹--波塔莫努特蟹(十足目:腕足动物:波塔莫努特蟹科)的证据","authors":"Kayleigh Mengel, Savel R Daniels","doi":"10.1093/jcbiol/ruae026","DOIUrl":null,"url":null,"abstract":"Specimens of freshwater crabs (PotamonautesMacLeay, 1838) collected from mountainous regions in the Limpopo and Mpumalanga provinces of South Africa could not be assigned to species level and possibly represented novel lineages. The phylogenetic placement of these two potential novel lineages were investigated with the use of DNA sequence data from three mitochondrial loci (cytochrome oxidase c subunit one - COI, 12S rRNA, and 16S rRNA) and one nuclear locus (histone 3 (H3)), using a maximum likelihood and Bayesian Inference approach incorporating all Potamonautes species described from southern Africa. In addition, a divergence time estimation was undertaken on the tree topology to determine the timing of cladogenesis. Morphometric analyses were also conducted on the two undescribed lineages and co-distributed, phylogenetically closely-related species (P. flavusjoDaniels, Phiri & Bayliss, 2014 and P. mariepskoppieDaniels, Barnes, Marais & Gouws, 2021) using discriminant function analyses of carapace and pereiopod variables. Phylogenetic results revealed that the two novel species are sister groups and related to a clade of endemic subtropical species present in north-eastern South Africa, while the divergence time estimation indicates Mio/Pliocene cladogenesis. The morphometric results corroborate the phylogenetic distinction between the two novel lineages and co-distributed sister species. Potamonautes wolkbergin. sp. and P. aurantiusn. sp. are described and compared with other congeneric South African taxa.","PeriodicalId":54850,"journal":{"name":"Journal of Crustacean Biology","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hiding in the hills: evidence for two novel mountain-dwelling freshwater crabs of Potamonautes (Decapoda: Brachyura: Potamonautidae) from South Africa\",\"authors\":\"Kayleigh Mengel, Savel R Daniels\",\"doi\":\"10.1093/jcbiol/ruae026\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Specimens of freshwater crabs (PotamonautesMacLeay, 1838) collected from mountainous regions in the Limpopo and Mpumalanga provinces of South Africa could not be assigned to species level and possibly represented novel lineages. The phylogenetic placement of these two potential novel lineages were investigated with the use of DNA sequence data from three mitochondrial loci (cytochrome oxidase c subunit one - COI, 12S rRNA, and 16S rRNA) and one nuclear locus (histone 3 (H3)), using a maximum likelihood and Bayesian Inference approach incorporating all Potamonautes species described from southern Africa. In addition, a divergence time estimation was undertaken on the tree topology to determine the timing of cladogenesis. Morphometric analyses were also conducted on the two undescribed lineages and co-distributed, phylogenetically closely-related species (P. flavusjoDaniels, Phiri & Bayliss, 2014 and P. mariepskoppieDaniels, Barnes, Marais & Gouws, 2021) using discriminant function analyses of carapace and pereiopod variables. Phylogenetic results revealed that the two novel species are sister groups and related to a clade of endemic subtropical species present in north-eastern South Africa, while the divergence time estimation indicates Mio/Pliocene cladogenesis. The morphometric results corroborate the phylogenetic distinction between the two novel lineages and co-distributed sister species. Potamonautes wolkbergin. sp. and P. aurantiusn. sp. are described and compared with other congeneric South African taxa.\",\"PeriodicalId\":54850,\"journal\":{\"name\":\"Journal of Crustacean Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Crustacean Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/jcbiol/ruae026\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Crustacean Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jcbiol/ruae026","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
从南非林波波省和姆普马兰加省山区采集的淡水蟹(PotamonautesMacLeay,1838 年)标本无法确定物种级别,可能代表新的品系。利用三个线粒体位点(细胞色素氧化酶 c 第一亚单位--COI、12S rRNA 和 16S rRNA)和一个核位点(组蛋白 3 (H3))的 DNA 序列数据,采用最大似然法和贝叶斯推断法,结合南部非洲描述的所有 Potamonautes 物种,研究了这两个潜在新品系的系统发育位置。此外,还对树的拓扑结构进行了分歧时间估计,以确定支系发生的时间。利用躯壳和围足变量的判别函数分析,还对两个未描述的品系和共同分布的、系统发育上密切相关的物种(P. flavusjoDaniels, Phiri & Bayliss, 2014 和 P. mariepskoppieDaniels, Barnes, Marais & Gouws, 2021)进行了形态计量分析。系统发育结果表明,这两个新物种是姊妹群,与南非东北部特有的亚热带物种支系有关,而分歧时间估计表明是中新世/更新世的支系发生。形态计量结果证实了这两个新品系与共同分布的姊妹种之间的系统发育差异。描述了Potamonautes wolkbergin.sp.和P. aurantiusn.sp.,并与南非其他同属类群进行了比较。
Hiding in the hills: evidence for two novel mountain-dwelling freshwater crabs of Potamonautes (Decapoda: Brachyura: Potamonautidae) from South Africa
Specimens of freshwater crabs (PotamonautesMacLeay, 1838) collected from mountainous regions in the Limpopo and Mpumalanga provinces of South Africa could not be assigned to species level and possibly represented novel lineages. The phylogenetic placement of these two potential novel lineages were investigated with the use of DNA sequence data from three mitochondrial loci (cytochrome oxidase c subunit one - COI, 12S rRNA, and 16S rRNA) and one nuclear locus (histone 3 (H3)), using a maximum likelihood and Bayesian Inference approach incorporating all Potamonautes species described from southern Africa. In addition, a divergence time estimation was undertaken on the tree topology to determine the timing of cladogenesis. Morphometric analyses were also conducted on the two undescribed lineages and co-distributed, phylogenetically closely-related species (P. flavusjoDaniels, Phiri & Bayliss, 2014 and P. mariepskoppieDaniels, Barnes, Marais & Gouws, 2021) using discriminant function analyses of carapace and pereiopod variables. Phylogenetic results revealed that the two novel species are sister groups and related to a clade of endemic subtropical species present in north-eastern South Africa, while the divergence time estimation indicates Mio/Pliocene cladogenesis. The morphometric results corroborate the phylogenetic distinction between the two novel lineages and co-distributed sister species. Potamonautes wolkbergin. sp. and P. aurantiusn. sp. are described and compared with other congeneric South African taxa.
期刊介绍:
The Journal of Crustacean Biology is the official journal of The Crustacean Society, publishing peer-reviewed research on all aspects of crustacean biology and other marine arthropods.
Papers are published in English only, but abstracts or summaries in French, German, Portuguese, or Spanish may be added when appropriate.