Patricia García Navas , María Yolanda Ruíz del Prado , Pablo Villoslada Blanco , Emma Recio Fernández , María Ruíz del Campo , Patricia Pérez Matute
{"title":"生长激素缺乏症患者治疗前后的微生物群组成","authors":"Patricia García Navas , María Yolanda Ruíz del Prado , Pablo Villoslada Blanco , Emma Recio Fernández , María Ruíz del Campo , Patricia Pérez Matute","doi":"10.1016/j.anpedi.2024.03.006","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>Growth hormone (GH) and insulin-like growth factor-1 (IGF-1) have modulatory effects on bowel function and its microbiota. Our aim was to investigate whether low levels of GH and IGF-1 in patients with GH deficiency are associated with changes in gut physiology/integrity as well as in the composition of the gut microbiota.</p></div><div><h3>Materials and methods</h3><p>We conducted a case-control study in 21 patients with GH deficiency, at baseline and after 6 months of GH treatment, and in 20 healthy controls. We analysed changes in anthropometric and laboratory characteristics and bacterial translocation and studied the composition of the microbiome by means of massive 16S rRNA gene sequencing.</p></div><div><h3>Results</h3><p>Growth hormone deficiency was accompanied by a significant increase in serum levels of sCD14, a marker of bacterial translocation (<em>P</em><.01). This increase was reversed by GH treatment. We did not find any differences in the composition or α- or β-diversity of the gut microbiota after treatment or between cases and controls.</p></div><div><h3>Conclusions</h3><p>Our work is the first to demonstrate that the presence of GH deficiency is not associated with differences in gut microbiota composition in comparison with healthy controls, and changes in microbiota composition are also not found after 6 months of treatment. However, GH deficiency and low IGF-1 levels were associated with an increase in bacterial translocation, which had reversed after treatment.</p></div>","PeriodicalId":7783,"journal":{"name":"Anales de pediatria","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1695403324000717/pdfft?md5=3ae42ceb14ed0ae583e4d320bd3614b8&pid=1-s2.0-S1695403324000717-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Composición de la microbiota en pacientes con déficit de hormona de crecimiento antes y después de recibir tratamiento\",\"authors\":\"Patricia García Navas , María Yolanda Ruíz del Prado , Pablo Villoslada Blanco , Emma Recio Fernández , María Ruíz del Campo , Patricia Pérez Matute\",\"doi\":\"10.1016/j.anpedi.2024.03.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>Growth hormone (GH) and insulin-like growth factor-1 (IGF-1) have modulatory effects on bowel function and its microbiota. Our aim was to investigate whether low levels of GH and IGF-1 in patients with GH deficiency are associated with changes in gut physiology/integrity as well as in the composition of the gut microbiota.</p></div><div><h3>Materials and methods</h3><p>We conducted a case-control study in 21 patients with GH deficiency, at baseline and after 6 months of GH treatment, and in 20 healthy controls. We analysed changes in anthropometric and laboratory characteristics and bacterial translocation and studied the composition of the microbiome by means of massive 16S rRNA gene sequencing.</p></div><div><h3>Results</h3><p>Growth hormone deficiency was accompanied by a significant increase in serum levels of sCD14, a marker of bacterial translocation (<em>P</em><.01). This increase was reversed by GH treatment. We did not find any differences in the composition or α- or β-diversity of the gut microbiota after treatment or between cases and controls.</p></div><div><h3>Conclusions</h3><p>Our work is the first to demonstrate that the presence of GH deficiency is not associated with differences in gut microbiota composition in comparison with healthy controls, and changes in microbiota composition are also not found after 6 months of treatment. 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Composición de la microbiota en pacientes con déficit de hormona de crecimiento antes y después de recibir tratamiento
Introduction
Growth hormone (GH) and insulin-like growth factor-1 (IGF-1) have modulatory effects on bowel function and its microbiota. Our aim was to investigate whether low levels of GH and IGF-1 in patients with GH deficiency are associated with changes in gut physiology/integrity as well as in the composition of the gut microbiota.
Materials and methods
We conducted a case-control study in 21 patients with GH deficiency, at baseline and after 6 months of GH treatment, and in 20 healthy controls. We analysed changes in anthropometric and laboratory characteristics and bacterial translocation and studied the composition of the microbiome by means of massive 16S rRNA gene sequencing.
Results
Growth hormone deficiency was accompanied by a significant increase in serum levels of sCD14, a marker of bacterial translocation (P<.01). This increase was reversed by GH treatment. We did not find any differences in the composition or α- or β-diversity of the gut microbiota after treatment or between cases and controls.
Conclusions
Our work is the first to demonstrate that the presence of GH deficiency is not associated with differences in gut microbiota composition in comparison with healthy controls, and changes in microbiota composition are also not found after 6 months of treatment. However, GH deficiency and low IGF-1 levels were associated with an increase in bacterial translocation, which had reversed after treatment.
期刊介绍:
La Asociación Española de Pediatría tiene como uno de sus objetivos principales la difusión de información científica rigurosa y actualizada sobre las distintas áreas de la pediatría. Anales de Pediatría es el Órgano de Expresión Científica de la Asociación y constituye el vehículo a través del cual se comunican los asociados. Publica trabajos originales sobre investigación clínica en pediatría procedentes de España y países latinoamericanos, así como artículos de revisión elaborados por los mejores profesionales de cada especialidad, las comunicaciones del congreso anual y los libros de actas de la Asociación, y guías de actuación elaboradas por las diferentes Sociedades/Secciones Especializadas integradas en la Asociación Española de Pediatría.