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引用次数: 0
摘要
本研究探讨了碱阳离子在调节脱氧核糖核酸(DNA)和硫黄素 T(ThT)在稀释和凝聚相中的相互作用中的作用。分析了 ThT 在双链 DNA 和 G-四链结构存在下的发射特性,重点研究了钠、钾、钙和镁四种阳离子的影响。双链 DNA 中的 ThT 发射受 DNA 直接结合和 DNA 水合壳内的立体阻碍的影响,而 DNA 水合壳内的立体阻碍又受碱阳离子存在的调节。激光光谱实验进一步突出了 ThT 对 DNA 水合外壳中水分子空间排列的敏感性。只有在 G 型四联结构中存在 Mg2+ 时才能观察到激光,这表明 G4 的平行螺旋桨构型为 ThT 光放大提供了最佳环境。这项研究强调了阳离子在 DNA 染料相互作用中的关键作用,并再次证实了 ThT 在生物物理研究中的重要性。
Role of Alkali Cations in DNA-Thioflavin T Interaction.
This study investigates the role of alkali cations in modulating the interaction between deoxyribonucleic acid (DNA) and Thioflavin T (ThT) in dilute and condensed phases. The emission characteristics of ThT were analyzed in the presence of double-stranded DNA and G-quadruplex structures with a focus on the effects of four cations: sodium, potassium, calcium, and magnesium. The ThT emission in double-stranded DNA was influenced by direct DNA binding and steric hindrance within the hydration shell of DNA, which was modulated by the presence of alkali cations. Lasing spectroscopy experiments further highlighted ThT sensitivity to the spatial arrangement of water molecules in the DNA hydration shell. Lasing was exclusively observed in the presence of Mg2+ in the G-quadruplex structure, suggesting that the parallel propeller configuration of G4 provides an optimal environment for ThT light amplification. This study highlights the critical role of cations in DNA-dye interactions and reaffirms the significance of ThT in biophysical studies.
期刊介绍:
An essential criterion for acceptance of research articles in the journal is that they provide new physical insight. Please refer to the New Physical Insights virtual issue on what constitutes new physical insight. Manuscripts that are essentially reporting data or applications of data are, in general, not suitable for publication in JPC B.