黑腹果蝇肠道转录组反应研究--敲除厄朗病毒的驯化诨基因 Gagr

IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Pub Date : 2024-06-05 DOI:10.1134/s0026893324700080
M. L. Nikitina, P. A. Milyaeva, I. V. Kuzmin, L. N. Nefedova
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摘要

摘要 黑腹果蝇(Drosophila melanogaster)基因组中的Gagr基因是二价病毒gag基因分子驯化的结果。以前的研究表明,相对于其他组织,Gagr基因在肠道组织中的转录水平最高,并且在雌性果蝇对营养培养基中添加的过硫酸铵的反应中,其转录被最有效地诱导。本研究对标准条件下和过硫酸铵胁迫条件下敲除 Gagr 基因的雌虫肠道各组织的转录组进行了研究。研究发现,在敲除 Gagr 基因的雌鼠肠道中,由 Toll 和 Imd 信号通路控制的抗菌肽基因被激活。通过过硫酸铵诱导应激反应,发现在敲除 Gagr 基因的蝇类中,JAK/STAT 和 JNK/MAPK 信号通路被破坏,ER-应激和 UPR-应激通路几乎完全没有激活。这些数据证实了Gagr基因在维持体内平衡和免疫反应中的重要作用。
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Study of the Gut Transcriptomic Response in Drosophila melanogaster with Knockdown of Gagr, Domesticated gag Gene of Errantiviruses

Abstract

As a result of molecular domestication of the gag gene of errantiviruses, the Gagr gene was formed in the genome of Drosophila melanogaster. It has previously been shown that the Gagr gene is transcribed at the highest level in gut tissues relative to other tissues, and its transcription is most effectively induced in females in response to ammonium persulfate added to the nutrient medium. In the present work, the gut transcriptome of females with knockdown of the Gagr gene was studied in all tissues under standard conditions and under stress conditions caused by ammonium persulfate. It was revealed that in females with knockdown of the Gagr gene, the genes of antimicrobial peptides controlled by the Toll and Imd signaling pathways are activated in the gut. Induction of a stress response by ammonium persulfate revealed disruption of the JAK/STAT and JNK/MAPK signaling pathways and an almost complete absence of activation of the ER-stress and UPR-stress pathways in flies with the Gagr gene knockdown. The data obtained confirm the important role of the Gagr gene in maintaining homeostasis and the immune response.

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来源期刊
Molecular Biology
Molecular Biology 生物-生化与分子生物学
CiteScore
1.30
自引率
8.30%
发文量
78
审稿时长
3 months
期刊介绍: Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.
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