CRISPR/Cas9 介导的基因干扰确定了 MITF 和 CITED2 在人类肥大细胞分化中的作用。

IF 7.4 1区 医学 Q1 HEMATOLOGY Blood advances Pub Date : 2024-08-13 DOI:10.1182/bloodadvances.2023012279
Jiezhen Mo, Fredrik Wermeling, Gunnar Nilsson, Joakim S Dahlin
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引用次数: 0

摘要

造血干细胞和祖细胞向各种细胞系的分化受细胞内在因素和祖细胞微环境的调控。小鼠实验已经确定了控制肥大细胞分化的转录调节因子。然而,目前还没有一种全面的方法能有效地破坏原代人类造血祖细胞中的单个转录调节因子,并同时读出肥大细胞的形成。在此,我们报告了一种简单的基于电穿孔和核糖核蛋白的基因敲除系统,该系统可识别人类肥大细胞分化所需和所不需的基因。我们发现转录因子 MITF 在人类肥大细胞祖细胞中上调,并揭示了 MITF 的缺失会导致肥大细胞的形成受到抑制。与此相反,另一个在肥大细胞分化过程中上调的转录调节因子 CITED2 对人类肥大细胞的分化却起着不可或缺的作用。综上所述,我们报告了一个基于 CRISPR/Cas9 的框架,该框架可用于识别参与调控人类肥大细胞形成的基因,其结果揭示了两个转录调节因子在控制人类肥大细胞分化中的作用。
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CRISPR/Cas9-mediated gene disruption determines the roles of MITF and CITED2 in human mast cell differentiation.
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来源期刊
Blood advances
Blood advances Medicine-Hematology
CiteScore
12.70
自引率
2.70%
发文量
840
期刊介绍: Blood Advances, a semimonthly medical journal published by the American Society of Hematology, marks the first addition to the Blood family in 70 years. This peer-reviewed, online-only, open-access journal was launched under the leadership of founding editor-in-chief Robert Negrin, MD, from Stanford University Medical Center in Stanford, CA, with its inaugural issue released on November 29, 2016. Blood Advances serves as an international platform for original articles detailing basic laboratory, translational, and clinical investigations in hematology. The journal comprehensively covers all aspects of hematology, including disorders of leukocytes (both benign and malignant), erythrocytes, platelets, hemostatic mechanisms, vascular biology, immunology, and hematologic oncology. Each article undergoes a rigorous peer-review process, with selection based on the originality of the findings, the high quality of the work presented, and the clarity of the presentation.
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