Single Jar for Collecting and Rearing Mosquito Life Stages: An Innovative Prototype.

Background objectives: Mosquitoes vectors are the key threat that spread viruses, bacteria, nematodes, protozoans, and other infections responsible for the transmission of serious public health ailments including dengue, yellow fever, malaria, chikungunya, etc.

Methods: To rear and cultivate mosquitoes at multiple stages of development (egg, larva, pupa, and adult) to be employed in various biomedical research, an innovative tool mosquito larva adult 2-in-1 rearing jar was designed and developed. The eggs/larvae or adults collected from the field or laboratory cultures can be released into the lower larvae or upper adult-rearing chamber so that they can find a suitable breeding place to oviposit or metamorphose into wrigglers and tumblers attaining the adult stage. Aedes, Anopheles, and Culex are three significant genera (Diptera: Culicidae) that were reared in experiments using this jar (Method I) in contrast to other conventional instruments available (Method II).

Results: Results of one-way ANOVA showed that the prototype was compatible and competitive for rearing as the data was non-significant when compared in both groups. However, throughout the trials, it was discovered that the prototype had a greater record of mean percentage of adult emergence (98.33), which supports the novelty of the instrument.

Interpretation conclusion: This unique device eliminates the need for multiple tools, causes minimum bodily damage to stages during handling, can be used in both the lab and the field, is inexpensive, lightweight, portable, and requires a single manpower to operate.