与成人相比,人类胎儿毛发发育过程中粘附分子(E-cadherin、β-catenin、CD56)和细胞角蛋白(CK19、CK20、HMWCK、CAM5.2)的免疫表达模式。

IF 1.1 4区 医学 Q4 DERMATOLOGY American Journal of Dermatopathology Pub Date : 2024-09-01 Epub Date: 2024-06-06 DOI:10.1097/DAD.0000000000002741
Özge Zorlu, Sevil Karabağ, Kıvılcım E Erdoğan, Merve Aksın, Batuhan Üstün
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引用次数: 0

摘要

摘要:细胞角蛋白或粘附分子表达异常与毛发疾病有关。这些分子在发育中的胎儿毛囊中的表达模式并不明显。我们旨在研究一些细胞角蛋白和粘附分子在人类胎儿毛囊中的表达模式,并与成人进行比较。我们选取了 48 例孕周大于 16 周的胎儿和 22 例接受良性痣或囊肿全切术的成人病例。皮肤样本取自胎儿的头皮和背部。对成人正常皮肤组织病理学区域进行了评估。进行了 CK19、CK20、CAM5.2、高分子量细胞角蛋白、E-cadherin、β-catenin 和 CD56 免疫组化染色。在胎儿组中,染色评分在妊娠三个月时下降,但在成人组中升高并达到最高水平,只有 CD56 除外,成人样本中没有染色。除 CD56 仅在胎儿中染色于毛周真皮鞘外,所有染色大多在根外鞘中观察到。E-cadherin、β-catenin和高分子量细胞角蛋白对所有成人样本都有强烈的弥漫性染色。胎儿(头皮得分:rs = 0.405,P = 0.004;背部得分:rs = 0.422,P = 0.003)和成人(背部得分:rs = 0.562,P = 0.046)的CAM5.2和CK19得分相关。CD56 阴性表明成人毛囊具有免疫特权特征。与 CK19 一样,CAM5.2 也可用于寻找干细胞或瞬时扩增细胞区域。
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Immunoexpression Patterns of Adhesion Molecules (E-cadherin, β-catenin, CD56) and Cytokeratins (CK19, CK20, HMWCK, CAM5.2) During Hair Development in Human Fetuses Compared With Adults.

Abstract: Abnormalities in the expression of cytokeratins or adhesion molecules have been associated with hair disorders. The expression patterns of these molecules in the hair follicles of developing human fetuses are not obvious. We aimed to investigate the expression patterns of some cytokeratins and adhesion molecules in the hair follicle of human fetuses and compared them with adults. Forty-eight fetuses of >16 gestational weeks and 22 adult cases with total excisions of benign nevi or cysts were enrolled. The skin samples were taken from both the scalp and back of the fetuses. The histopathologically normal skin areas were evaluated in adults. CK19, CK20, CAM5.2, high-molecular-weight cytokeratin, E-cadherin, β-catenin, and CD56 immunohistochemical stainings were performed. In the fetus group, the staining scores declined in the third trimester but elevated and reached the highest level in adults, except for CD56, which did not stain any adult samples. All stainings were mostly observed in the outer root sheath, except CD56 that stained the perifollicular dermal sheath only in fetuses. E-cadherin, β-catenin, and high-molecular-weight cytokeratin strongly and diffusely stained all adult samples. CAM5.2 and CK19 scores were correlated in fetuses (scalp scores: r s = 0.405, P = 0.004; back scores: r s = 0.422, P = 0.003) and adults (back scores: r s = 0.562, P = 0.046). CD56 negativity indicated the immune-privilege feature of adult hair follicles. As CK19, CAM5.2 may be used to find the regions of stem cells or transient amplifying cells.

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来源期刊
CiteScore
1.80
自引率
9.10%
发文量
453
审稿时长
3 months
期刊介绍: The American Journal of Dermatopathology offers outstanding coverage of the latest diagnostic approaches and laboratory techniques, as well as insights into contemporary social, legal, and ethical concerns. Each issue features review articles on clinical, technical, and basic science advances and illuminating, detailed case reports. With the The American Journal of Dermatopathology you''ll be able to: -Incorporate step-by-step coverage of new or difficult-to-diagnose conditions from their earliest histopathologic signs to confirmatory immunohistochemical and molecular studies. -Apply the latest basic science findings and clinical approaches to your work right away. -Tap into the skills and expertise of your peers and colleagues the world over peer-reviewed original articles, "Extraordinary cases reports", coverage of practical guidelines, and graphic presentations. -Expand your horizons through the Journal''s idea-generating forum for debating controversial issues and learning from preeminent researchers and clinicians
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