Multiple Sampling Capsule Robot for Studying Gut Microbiome

Longitudinal analysis of the gut microbiota is crucial for understanding its relationship with gastrointestinal (GI) diseases and advancing diagnostics and treatments. Most ingestible sampling devices move passively within the GI tract, rely on physiological factors, and fail at multipoint sampling. This study proposes a multiple‐sampling capsule robot capable of collecting gut microbiota from various locations within the GI tract with minimal cross‐contamination. The proposed capsule comprises a body, a driving unit, six sampling tools, a central rod, and two heads. Electromagnetic field control facilitates control of the orientation and position of the capsule, particularly to align the channel of the capsule where the sample is collected facing downward. The capsule can collect six gut microbiota samples preventing contamination before and after sampling. The active locomotion and multiple sampling performance of the capsule are evaluated through basic performance tests (propulsion direction precision: 0.76 ± 0.52°, channel alignment precision: 0.84 ± 0.55°), phantom tests (average amount per sample: 10.3 ± 2.4 mg, cross‐contamination: 0.6 ± 0.4%), and ex‐vivo tests (average amount per sample: 9.9 ± 1.7 mg). The possibility of integration and clinical application of the capsule is confirmed through preclinical tests using a porcine model.