肾小球内皮细胞受体 ADGRF5 与肾小球滤过屏障的完整性

IF 10.3 1区 医学 Q1 UROLOGY & NEPHROLOGY Journal of The American Society of Nephrology Pub Date : 2024-10-01 Epub Date: 2024-06-06 DOI:10.1681/ASN.0000000000000427
Miki Nagase, Hikaru Ando, Yoshiaki Beppu, Hidetake Kurihara, Souta Oki, Fumimasa Kubo, Kazuki Yamamoto, Takashi Nagase, Shinya Kaname, Yoshihiro Akimoto, Hiroshi Fukuhara, Tatsuo Sakai, Shigehisa Hirose, Nobuhiro Nakamura
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引用次数: 0

摘要

背景:肾小球内皮细胞被认为是维持肾小球滤过屏障的重要细胞。ADGRF5 是一种粘附 G 蛋白偶联受体,被认为参与了内皮细胞的功能。然而,ADGRF5在肾小球滤过屏障完整性中的作用仍不明确:方法:通过组织学分析确定ADGRF5在小鼠肾小球中的细胞表达。然后利用 ADGRF5 基因敲除(Adgrf5-/-)小鼠和人类原代肾小球内皮细胞分析了 ADGRF5 缺失对肾小球形态、肾功能和肾小球内皮基因/蛋白表达的影响:结果:ADGRF5在肾小球内的毛细血管内皮细胞中特异性表达。Adgrf5-/-小鼠出现白蛋白尿,肾功能受损,肾小球形态学缺陷,即肾小球肥大、肾小球基底膜裂开和增厚、肾小球内皮细胞膈膜开裂和脱落以及系膜穿插。伴随这些缺陷的是肾小球内皮细胞中负责肾小球基底膜组织的基因(IV型胶原和层粘连蛋白)和Krüppel样因子2(Klf2)表达的改变。此外,在人原代肾小球内皮细胞中,ADGRF5的敲除降低了COL4A3和COL4A4的表达,增加了KLF2的表达:结论:ADGRF5的缺失会导致肾小球内皮细胞基因表达的改变,并扰乱肾小球滤过屏障的结构和选择性。
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Glomerular Endothelial Cell Receptor Adhesion G-Protein-Coupled Receptor F5 (ADGRF5) and the Integrity of the Glomerular Filtration Barrier.
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来源期刊
Journal of The American Society of Nephrology
Journal of The American Society of Nephrology 医学-泌尿学与肾脏学
CiteScore
22.40
自引率
2.90%
发文量
492
审稿时长
3-8 weeks
期刊介绍: The Journal of the American Society of Nephrology (JASN) stands as the preeminent kidney journal globally, offering an exceptional synthesis of cutting-edge basic research, clinical epidemiology, meta-analysis, and relevant editorial content. Representing a comprehensive resource, JASN encompasses clinical research, editorials distilling key findings, perspectives, and timely reviews. Editorials are skillfully crafted to elucidate the essential insights of the parent article, while JASN actively encourages the submission of Letters to the Editor discussing recently published articles. The reviews featured in JASN are consistently erudite and comprehensive, providing thorough coverage of respective fields. Since its inception in July 1990, JASN has been a monthly publication. JASN publishes original research reports and editorial content across a spectrum of basic and clinical science relevant to the broad discipline of nephrology. Topics covered include renal cell biology, developmental biology of the kidney, genetics of kidney disease, cell and transport physiology, hemodynamics and vascular regulation, mechanisms of blood pressure regulation, renal immunology, kidney pathology, pathophysiology of kidney diseases, nephrolithiasis, clinical nephrology (including dialysis and transplantation), and hypertension. Furthermore, articles addressing healthcare policy and care delivery issues relevant to nephrology are warmly welcomed.
期刊最新文献
Authors' Reply: Utility of Urinary CD4 + T-Cell Count in Detecting ANCA-Associated Vasculitis Renal Relapse. Glomerular Endothelial Cell Receptor Adhesion G-Protein-Coupled Receptor F5 (ADGRF5) and the Integrity of the Glomerular Filtration Barrier. P2Y2R and Cyst Growth in Polycystic Kidney Disease. The Plasma Metabolome and Risk of Incident Kidney Stones. Association between Acute Declines in eGFR during Renin-Angiotensin System Inhibition and Risk of Adverse Outcomes.
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