孟加拉国圈养白虎的基因组测序。

IF 1.9 Q3 GENETICS & HEREDITY BMC genomic data Pub Date : 2024-06-06 DOI:10.1186/s12863-024-01239-5
Ashutosh Das, Md Shahadat Hossain Suvo, Mishuk Shaha, Mukta Das Gupta
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引用次数: 0

摘要

目标孟加拉虎(Panthera tigris tigris)是孟加拉国的标志性动物。尽管孟加拉虎是野生动物中的顶级掠食者,但由于人类活动,如狩猎、城市化、农业扩张和森林砍伐,孟加拉虎的数量正在减少。相比之下,人工饲养的老虎却因切实可行的保护工作而蓬勃发展。在圈养的小种群中繁殖会产生近亲繁殖抑制和遗传瓶颈,这可能会限制保护工作的成功。尽管已经进行了几十年的研究,但孟加拉国圈养和野生孟加拉虎遗传变异的综合数据库仍有待建立。因此,本研究旨在调查白孟加拉虎基因组,为今后的研究建立一个资源库,以了解重要功能特征的基础变异:数据描述:从 Chattogram 动物园采集了三只孟加拉白虎的血液样本。使用商业 DNA 提取试剂盒提取了所有采集样本的基因组 DNA。使用 DNBseq 平台进行了全基因组测序。我们为三只孟加拉虎生成了 77 Gb 的全基因组测序(WGS)数据(平均 11 倍覆盖率/样本)。我们生成的数据将为孟加拉白虎未来的功能研究提供基因组资源,从而为孟加拉国的老虎研究建立一个范例。
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Genome sequencing of captive white tigers from Bangladesh.

Objectives: The Bengal tiger Panthera tigris tigris, is an emblematic animal for Bangladesh. Despite being the apex predator in the wild, their number is decreasing due to anthropogenic activities such as hunting, urbanization, expansion of agriculture and deforestation. By contrast, captive tigers are flourishing due to practical conservation efforts. Breeding within the small captive population can produce inbreeding depression and genetic bottlenecks, which may limit the success of conservation efforts. Despite past decades of research, a comprehensive database on genetic variation in the captive and wild Bengal tigers in Bangladesh still needs to be included. Therefore, this research aimed to investigate the White Bengal tiger genome to create a resource for future studies to understand variation underlying important functional traits.

Data description: Blood samples from Chattogram Zoo were collected for three white Bengal tigers. Genomic DNA for all collected samples were extracted using a commercial DNA extraction kit. Whole genome sequencing was performed using a DNBseq platform. We generated 77 Gb of whole-genome sequencing (WGS) data for three white Bengal tigers (Average 11X coverage/sample). The data we generated will establish a paradigm for tiger research in Bangladesh by providing a genomic resource for future functional studies on the Bengal white tiger.

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