苍术素通过下调 microRNA-214-5p 减轻炎症,从而缓解脓毒症相关的心功能不全。

IF 2.2 4区 医学 Q3 TOXICOLOGY Toxicology Research Pub Date : 2024-06-07 eCollection Date: 2024-06-01 DOI:10.1093/toxres/tfae081
Chunyan Li, Daorong Hou, Yanhong Huang, Yifan Liu, Yong Li, Cheng Wang
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引用次数: 0

摘要

背景:然而,堇菜素对脓毒症相关心功能障碍的影响仍不清楚。本研究的目的是确定苍术素在败血症相关心功能障碍中的作用和机制:方法:对小鼠进行脂多糖(LPS)或盲肠结扎和穿刺(CLP)诱导的败血症或 LPS 诱导的心肌细胞败血症实验:结果:服用苍术素能改善 LPS 或 CLP 诱导的小鼠心脏功能障碍。紫杉醇能抑制 LPS 或 CLP 诱导的小鼠心脏中白细胞介素-1(IL)-1β、IL-6 和肿瘤坏死因子(TNF)-α 的增加。LPS 可使心肌细胞中的 IL-1β、IL-6 和 TNF-α 水平升高,而 Corylin 处理可抑制这些升高。在使用 LPS、CLP、LPS 处理的 NRCMs、H9c2 和 AC16 细胞的小鼠心脏中,Corylin 可抑制 microRNA (miRNA)-214-5p 的增加。服用 miRNA-214-5p agomiR 逆转了苍术素对 LPS 治疗小鼠受损心脏功能的改善作用以及 IL-1β、IL-6 和 TNF-α 的增加:结论:这些结果表明,鼠尾草素通过抑制炎症改善了败血症相关的心脏功能障碍。结论:这些结果表明,毛果芸香碱通过抑制炎症改善了败血症引起的心脏功能障碍,而毛果芸香碱通过降低 miRNA-214-5p 抑制了败血症的炎症反应。下调 miRNA-214-5p 可改善脓毒症相关的心功能障碍并抑制炎症因子。
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Corylin alleviated sepsis-associated cardiac dysfunction via attenuating inflammation through downregulation of microRNA-214-5p.

Background: Corylin, a natural flavonoid, is isolated from the fruit of Psoralea corylifolia L. Nevertheless, the effect of corylin on sepsis-associated cardiac dysfunction is still unclear. The purpose of this study is to determine the role and mechanism of corylin in sepsis related cardiac dysfunction.

Methods: Experiments were carried out on mice with lipopolysaccharide (LPS) or sepsis induced by cecal ligation and puncture (CLP) or myocardial cell sepsis induced by LPS.

Results: Administration of corylin improved cardiac dysfunction induced by LPS or CLP in mice. Corylin inhibited the increases of interleukin-1 (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α in the heart of mice with LPS or CLP. LPS elevated the levels of IL-1β, IL-6 and TNF-α in cardiomyocytes, which were inhibited by corylin treatment. Corylin attenuated the increases of microRNA (miRNA)-214-5p in the heart of mice with LPS, CLP, LPS-treated NRCMs, H9c2 and AC16 cells. Administration of miRNA-214-5p agomiR reversed the improving effects of corylin on the damaged cardiac function and the increases of IL-1β, IL-6 and TNF-α in mice treated with LPS.

Conclusion: These outcomes indicated that corylin improved sepsis-associated cardiac dysfunction by inhibiting inflammation. And corylin inhibited inflammation of sepsis by decreasing miRNA-214-5p. Downregulation of miRNA-214-5p improved sepsis-associated cardiac dysfunction and inhibited inflammatory factors.

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Toxicology Research
Toxicology Research TOXICOLOGY-
CiteScore
3.60
自引率
0.00%
发文量
82
期刊介绍: A multi-disciplinary journal covering the best research in both fundamental and applied aspects of toxicology
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