Rafael Dascanio DDS, MS, Rafael Antonio de Oliveira Ribeiro DDS, MS, Camila Siqueira Silva Coelho DDS, MS, PhD, Marina Trevelin Souza DDS, MS, PhD, Matheus Kury DDS, MS, PhD, Edgar Dutra Zanotto Eng, MS, PhD, Carlos Alberto de Souza Costa DDS, MS, PhD, Vanessa Cavalli DDS, MS, PhD
{"title":"生物硅酸盐强化漂白凝胶对早期侵蚀病变珐琅质的有效性和安全性。","authors":"Rafael Dascanio DDS, MS, Rafael Antonio de Oliveira Ribeiro DDS, MS, Camila Siqueira Silva Coelho DDS, MS, PhD, Marina Trevelin Souza DDS, MS, PhD, Matheus Kury DDS, MS, PhD, Edgar Dutra Zanotto Eng, MS, PhD, Carlos Alberto de Souza Costa DDS, MS, PhD, Vanessa Cavalli DDS, MS, PhD","doi":"10.1111/jerd.13271","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aim</h3>\n \n <p>This study evaluated the efficacy and cytotoxicity of 35% hydrogen peroxide (HP) gel incorporated with 10% (w/w) biosilicate (BioS) on sound enamel and early-stage enamel erosion lesions.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Discs of enamel/dentin were selected, subjected to erosive cycles (0.3% citric acid, pH 2.6), and treated with (<i>n</i> = 8): HP (35% HP, positive control); HP_BioS [carboxymethyl cellulose (CMC) + HP + BioS]; BioS (CMC + BioS); CMC (negative control). The discs were adapted to artificial pulp chambers with the enamel exposed for bleaching, and the dentin facing toward the culture medium (Dulbecco's modified Eagle's medium [DMEM]). Bleaching was performed in three 30-min sessions at 7-day intervals. After bleaching, the diffusion product (DMEM extract + diffused HP) was pipetted onto MDPC-23 odontoblastic cell line and inoculated. Color parameters (Δ<i>L</i>, Δ<i>a</i>, Δ<i>b</i>), color change (Δ<i>E</i><sub>00</sub>), and changes in whiteness index (ΔWI<sub>D</sub>) were determined before (<i>T</i><sub>0</sub>) and after the last bleaching session (<i>T</i><sub>3</sub>). Cell viability (MTT, %), H<sub>2</sub>O<sub>2</sub> diffusion (μg/mL), oxidative cell stress (OxS), and cell fluorescence (live/dead assay, in confocal microscopy) were assessed (ANOVA/Tukey; <i>α</i> = 0.05).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>No difference in Δ<i>L</i>, Δ<i>a</i>, Δ<i>b</i>, Δ<i>E</i><sub>00</sub>, and ΔWI<sub>D</sub> were found between HP and HP_BioS (<i>p</i> > 0.05). The incorporation of BioS decreased the HP diffusion into the substrates and mitigated oxidative stress in early-stage eroded enamel (<i>p</i> < 0.05). HP_BioS presented significantly higher cell viability compared with HP under erosion conditions. Live/dead assay indicated that BioS_HP maintained viability with larger clusters of viable cells.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Incorporating BioS into HP maintained bleaching effectiveness, favored cell viability, reduced the oxidative stress, and the cytotoxicity in teeth with early-stage erosion.</p>\n </section>\n \n <section>\n \n <h3> Clinical Significance</h3>\n \n <p>BioS formulation showed promising results for reducing cytotoxicity in patients seeking tooth bleaching and presenting undetectable early-stage erosion.</p>\n </section>\n </div>","PeriodicalId":15988,"journal":{"name":"Journal of Esthetic and Restorative Dentistry","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effectiveness and safety of biosilicate-enhanced bleaching gels on enamel with early erosion lesion\",\"authors\":\"Rafael Dascanio DDS, MS, Rafael Antonio de Oliveira Ribeiro DDS, MS, Camila Siqueira Silva Coelho DDS, MS, PhD, Marina Trevelin Souza DDS, MS, PhD, Matheus Kury DDS, MS, PhD, Edgar Dutra Zanotto Eng, MS, PhD, Carlos Alberto de Souza Costa DDS, MS, PhD, Vanessa Cavalli DDS, MS, PhD\",\"doi\":\"10.1111/jerd.13271\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aim</h3>\\n \\n <p>This study evaluated the efficacy and cytotoxicity of 35% hydrogen peroxide (HP) gel incorporated with 10% (w/w) biosilicate (BioS) on sound enamel and early-stage enamel erosion lesions.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Discs of enamel/dentin were selected, subjected to erosive cycles (0.3% citric acid, pH 2.6), and treated with (<i>n</i> = 8): HP (35% HP, positive control); HP_BioS [carboxymethyl cellulose (CMC) + HP + BioS]; BioS (CMC + BioS); CMC (negative control). The discs were adapted to artificial pulp chambers with the enamel exposed for bleaching, and the dentin facing toward the culture medium (Dulbecco's modified Eagle's medium [DMEM]). Bleaching was performed in three 30-min sessions at 7-day intervals. After bleaching, the diffusion product (DMEM extract + diffused HP) was pipetted onto MDPC-23 odontoblastic cell line and inoculated. Color parameters (Δ<i>L</i>, Δ<i>a</i>, Δ<i>b</i>), color change (Δ<i>E</i><sub>00</sub>), and changes in whiteness index (ΔWI<sub>D</sub>) were determined before (<i>T</i><sub>0</sub>) and after the last bleaching session (<i>T</i><sub>3</sub>). Cell viability (MTT, %), H<sub>2</sub>O<sub>2</sub> diffusion (μg/mL), oxidative cell stress (OxS), and cell fluorescence (live/dead assay, in confocal microscopy) were assessed (ANOVA/Tukey; <i>α</i> = 0.05).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>No difference in Δ<i>L</i>, Δ<i>a</i>, Δ<i>b</i>, Δ<i>E</i><sub>00</sub>, and ΔWI<sub>D</sub> were found between HP and HP_BioS (<i>p</i> > 0.05). The incorporation of BioS decreased the HP diffusion into the substrates and mitigated oxidative stress in early-stage eroded enamel (<i>p</i> < 0.05). HP_BioS presented significantly higher cell viability compared with HP under erosion conditions. Live/dead assay indicated that BioS_HP maintained viability with larger clusters of viable cells.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Incorporating BioS into HP maintained bleaching effectiveness, favored cell viability, reduced the oxidative stress, and the cytotoxicity in teeth with early-stage erosion.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Clinical Significance</h3>\\n \\n <p>BioS formulation showed promising results for reducing cytotoxicity in patients seeking tooth bleaching and presenting undetectable early-stage erosion.</p>\\n </section>\\n </div>\",\"PeriodicalId\":15988,\"journal\":{\"name\":\"Journal of Esthetic and Restorative Dentistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-06-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Esthetic and Restorative Dentistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jerd.13271\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Esthetic and Restorative Dentistry","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jerd.13271","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Effectiveness and safety of biosilicate-enhanced bleaching gels on enamel with early erosion lesion
Aim
This study evaluated the efficacy and cytotoxicity of 35% hydrogen peroxide (HP) gel incorporated with 10% (w/w) biosilicate (BioS) on sound enamel and early-stage enamel erosion lesions.
Methods
Discs of enamel/dentin were selected, subjected to erosive cycles (0.3% citric acid, pH 2.6), and treated with (n = 8): HP (35% HP, positive control); HP_BioS [carboxymethyl cellulose (CMC) + HP + BioS]; BioS (CMC + BioS); CMC (negative control). The discs were adapted to artificial pulp chambers with the enamel exposed for bleaching, and the dentin facing toward the culture medium (Dulbecco's modified Eagle's medium [DMEM]). Bleaching was performed in three 30-min sessions at 7-day intervals. After bleaching, the diffusion product (DMEM extract + diffused HP) was pipetted onto MDPC-23 odontoblastic cell line and inoculated. Color parameters (ΔL, Δa, Δb), color change (ΔE00), and changes in whiteness index (ΔWID) were determined before (T0) and after the last bleaching session (T3). Cell viability (MTT, %), H2O2 diffusion (μg/mL), oxidative cell stress (OxS), and cell fluorescence (live/dead assay, in confocal microscopy) were assessed (ANOVA/Tukey; α = 0.05).
Results
No difference in ΔL, Δa, Δb, ΔE00, and ΔWID were found between HP and HP_BioS (p > 0.05). The incorporation of BioS decreased the HP diffusion into the substrates and mitigated oxidative stress in early-stage eroded enamel (p < 0.05). HP_BioS presented significantly higher cell viability compared with HP under erosion conditions. Live/dead assay indicated that BioS_HP maintained viability with larger clusters of viable cells.
Conclusion
Incorporating BioS into HP maintained bleaching effectiveness, favored cell viability, reduced the oxidative stress, and the cytotoxicity in teeth with early-stage erosion.
Clinical Significance
BioS formulation showed promising results for reducing cytotoxicity in patients seeking tooth bleaching and presenting undetectable early-stage erosion.
期刊介绍：
The Journal of Esthetic and Restorative Dentistry (JERD) is the longest standing peer-reviewed journal devoted solely to advancing the knowledge and practice of esthetic dentistry. Its goal is to provide the very latest evidence-based information in the realm of contemporary interdisciplinary esthetic dentistry through high quality clinical papers, sound research reports and educational features.
The range of topics covered in the journal includes:
- Interdisciplinary esthetic concepts
- Implants
- Conservative adhesive restorations
- Tooth Whitening
- Prosthodontic materials and techniques
- Dental materials
- Orthodontic, periodontal and endodontic esthetics
- Esthetics related research
- Innovations in esthetics
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