评估新型内生真菌 Chaetomium ascotrichoides 1-24-2 作为一种生物防治剂对由嗜木刺孢子虫引起的松树枯萎病的防治效果。

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-06-11 DOI:10.1002/ps.8205

Md Kamaruzzaman, Lijun Zheng, Shun Zhou, Wenhua Ye, Yongqiang Yuan, Qiu Qi, Yongfeng Gao, Jiajin Tan, Yan Wang, Bingjia Chen, Zhiguang Li, Songsong Liu, Renjun Mi, Ke Zhang, Chen Zhao, Waqar Ahmed, Xinrong Wang

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Based on the morphological and molecular characteristics, the isolated strain 1-24-2 was identified as Chaetomium ascotrichoides. In the in-planta assay, pine seedlings (2-years-old) treated with 1-24-2 CFs + pine wood nematode (T2) showed a significant control effect of 80%. A total of 24 toxic compounds were first identified from 1-24-2 CFs through gas chromatography-mass spectrometry (GC-MS) analysis, from which O-methylisourea, 2-chlorobenzothiazole, and 4,5,6-trihydroxy-7-methylphthalide showed robust binding sites at Tyr119 against phosphoethanolamine methyltransferase (PMT) protein of Bursaphelenchus xylophilus by molecular docking approach and could be used as potential compounds for developing effective nematicides. 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引用次数: 0

摘要

背景：松树枯萎病（PWD）的病原菌嗜木囊霉菌（Bursaphelenchus xylophilus）对全球松树林的威胁与日俱增。本研究旨在通过应用从健康松树中分离的内生真菌来开发松树枯萎病的生物防治方法：结果：我们成功地从健康松树枝条中分离出了一种新型内生真菌菌株 1-24-2。菌株 1-24-2 的培养滤液（CFs）对嗜线毛囊虫（Bursaphelenchus xylophilus）具有很强的杀线虫活性，校正死亡率为 99.00%。根据形态学和分子特征，分离出的菌株 1-24-2 被鉴定为蛔虫链格孢菌（Chaetomium ascotrichoides）。在植物体内试验中，用 1-24-2 CFs + 松材线虫（T2）处理的松树苗（2 年生）显示出 80% 的显著防治效果。通过气相色谱-质谱（GC-MS）分析，首次从 1-24-2 CFs 中鉴定出 24 种有毒化合物，其中 O-甲基异脲、2-氯苯并噻唑和 4,5,6-三羟基-7-甲基邻苯二甲酸酯通过分子对接方法在 Tyr119 处显示出与嗜木刺孢子虫磷乙醇胺甲基转移酶（PMT）蛋白的强结合位点，可作为开发有效杀线虫剂的潜在化合物。有趣的是，菌株 1-24-2 会产生有毒的挥发性有机化合物（VOCs），从而干扰嗜木刺吸虫的自然生长过程，与对照组相比，处理组的嗜木刺吸虫总数减少了 83.32%，同时还减少了灰霉病菌的生长，降幅高达 71.01%：我们的研究结果凸显了 C. ascotrichoides 1-24-2 作为一种有潜力的生物防治剂的潜力，它对嗜木虱具有很强的杀线虫活性。这是世界上首次报道从 P. massoniana 中分离出的 C. ascotrichoides 对嗜木虱具有很强的生物防治潜力。© 2024 化学工业学会。

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Evaluation of the novel endophytic fungus Chaetomium ascotrichoides 1-24-2 from Pinus massoniana as a biocontrol agent against pine wilt disease caused by Bursaphelenchus xylophilus.

Background: Bursaphelenchus xylophilus, the causative agent of pine wilt disease (PWD), is an ever-increasing threat to Pinus forests worldwide. This study aimed to develop biological control of PWD by the application of endophytic fungi isolated from healthy pine trees.

Results: We successfully isolated a novel endophytic fungal strain 1-24-2 from branches of healthy Pinus massoniana. The culture filtrates (CFs) of strain 1-24-2 exhibited strong nematicidal activity against Bursaphelenchus xylophilus, with a corrected mortality rate of 99.00%. Based on the morphological and molecular characteristics, the isolated strain 1-24-2 was identified as Chaetomium ascotrichoides. In the in-planta assay, pine seedlings (2-years-old) treated with 1-24-2 CFs + pine wood nematode (T2) showed a significant control effect of 80%. A total of 24 toxic compounds were first identified from 1-24-2 CFs through gas chromatography-mass spectrometry (GC-MS) analysis, from which O-methylisourea, 2-chlorobenzothiazole, and 4,5,6-trihydroxy-7-methylphthalide showed robust binding sites at Tyr119 against phosphoethanolamine methyltransferase (PMT) protein of Bursaphelenchus xylophilus by molecular docking approach and could be used as potential compounds for developing effective nematicides. Interestingly, strain 1-24-2 produces toxic volatile organic compounds (VOCs), which disturb the natural development process of B. xylophilus, whose total number decreased by up to 83.32% in the treatment group as compared to control and also reduced Botrytis cinerea growth by up to 71.01%.

Conclusion: Our results highlight the potential of C. ascotrichoides 1-24-2 as a promising biocontrol agent with solid nematicidal activity against B. xylophilus. This is the first report of C. ascotrichoides isolated from P. massoniana exhibiting strong biocontrol potential against B. xylophilus in the world. © 2024 Society of Chemical Industry.

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.