Annie Porter, Emily Newcomb, Steven DiStefano, Jacob Poplawski, Jonathan Kim, Michael Axe, Xin Lucas Lu
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Following a short- (2 days) or long-term (10–14 days) treatment, chondrocyte viability, proliferation, and extracellular matrix (ECM) synthesis and degradation were evaluated with a click chemistry-based technique. Chondrocyte viability, proliferation, and anabolic activity were all minimally affected by short-term and long-term TA treatment. After both acute and sustained inflammatory challenges, TA reduced the catabolic activities in cartilage, reducing nascent glycosaminoglycan loss and maintaining cartilage mechanical properties. Overall, at physiologically relevant doses, TA had minimal negative impact on chondrocytes when maintained within their native ECM. 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引用次数: 0
摘要
临床医生通常使用关节内皮质类固醇注射(如曲安奈德)来控制关节滑膜炎症。然而,由于文献中的证据相互矛盾,临床医生担心注射可能会对年轻患者原本健康的软骨造成伤害。本研究旨在评估 TA 对年轻健康软骨细胞的影响。关节软骨样本取自牛膝关节(1-2 个月大)。在健康软骨和受到炎症(白细胞介素-1β)挑战的软骨中,用 1 nM 至 200 μM 剂量的 TA 处理样本。经过短期(2 天)或长期(10-14 天)处理后,采用点击化学技术对软骨细胞的活力、增殖以及细胞外基质(ECM)的合成和降解进行了评估。软骨细胞的活力、增殖和同化活性均受到短期和长期 TA 处理的最小影响。在急性和持续的炎症挑战后,TA都能降低软骨的分解代谢活动,减少新生糖胺聚糖的损失,保持软骨的机械性能。总之,在生理相关剂量下,当软骨细胞保持在其原生 ECM 中时,TA 对软骨细胞的负面影响微乎其微。临床意义:这些研究结果为目前在关节内注射中使用TA的临床实践提供了新的见解,尤其是在年轻患者中,并为今后研究皮质类固醇对关节稳态的影响奠定了基础。
Triamcinolone acetonide has minimal effect on short- and long-term metabolic activities of cartilage
Intra-articular corticosteroid injections, such as triamcinolone acetonide (TA), are commonly used by clinicians to manage joint synovial inflammation. However, due to conflicting evidence in literature, there is a fear among clinicians that the injections may be harmful to otherwise healthy cartilage in young patients. The purpose of this study was to evaluate the effects of TA on young, healthy chondrocytes. Articular cartilage samples were harvested from bovine knee joints (1–2 months old). In both healthy and inflammatory (interleukin-1β) challenged cartilage, samples were treated with TA at doses ranging from 1 nM to 200 μM. Following a short- (2 days) or long-term (10–14 days) treatment, chondrocyte viability, proliferation, and extracellular matrix (ECM) synthesis and degradation were evaluated with a click chemistry-based technique. Chondrocyte viability, proliferation, and anabolic activity were all minimally affected by short-term and long-term TA treatment. After both acute and sustained inflammatory challenges, TA reduced the catabolic activities in cartilage, reducing nascent glycosaminoglycan loss and maintaining cartilage mechanical properties. Overall, at physiologically relevant doses, TA had minimal negative impact on chondrocytes when maintained within their native ECM. Clinical significance: The findings provide new insight for current clinical practices concerning the use of TA in intra-articular injections, especially in young patients, and established a foundation for future investigations into the impact of corticosteroids on joint homeostasis.
期刊介绍:
The Journal of Orthopaedic Research is the forum for the rapid publication of high quality reports of new information on the full spectrum of orthopaedic research, including life sciences, engineering, translational, and clinical studies.