Waqas Hussain Shah, Wajiha Khan, Sobia Nisa, Michael H J Barfuss, Johann Schinnerl, Markus Bacher, Karin Valant-Vetschera, Ashraf Ali, Hiba-Allah Nafidi, Yousef A Bin Jardan, John P Giesy
{"title":"具有治疗活性的真菌内生菌中化学成分的高效液相色谱和核磁共振表征、抗氧化和抗癌活性。","authors":"Waqas Hussain Shah, Wajiha Khan, Sobia Nisa, Michael H J Barfuss, Johann Schinnerl, Markus Bacher, Karin Valant-Vetschera, Ashraf Ali, Hiba-Allah Nafidi, Yousef A Bin Jardan, John P Giesy","doi":"10.4014/jmb.2403.03036","DOIUrl":null,"url":null,"abstract":"<p><p>Fungi generate different metabolites some of which are intrinsically bioactive and could therefore serve as templates for drug development. In the current study, six endophytic fungi namely <i>Aspergillus flavus</i>, <i>Aspergillus tubigenesis</i>, <i>Aspergillus oryzae</i>, <i>Penicillium oxalicum</i>, <i>Aspergillus niger</i>, and <i>Aspergillus brasiliensis</i> were isolated and identified from the medicinal plant, <i>Silybum marianum</i>. These endophytic fungi were identified through intra transcribed sequence (ITS) gene sequencing. The bioactive potentials of fungal extracts were investigated using several bioassays such as antibacterial activity by well-diffusion, MIC, MBC, anti-biofilm, antioxidant, and haemolysis. The <i>Pseudomonas aeruginosa</i> PAO1 was used to determine the antibiofilm activity. The ethyl acetate extract of <i>Aspergillus flavus</i> showed strong to moderate efficacy against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, <i>P. aeruginosa</i>, and <i>Bacillus spizizenii</i>. <i>Aspergillus flavus</i> and <i>Aspergillus brasiliensis</i> exhibited significant antibiofilm activity with IC<sub>50</sub> at 4.02 and 3.63 mg/ml, while <i>A. flavus</i> exhibited maximum antioxidant activity of 50.8%. Based on HPLC, LC-MS, and NMR experiments kojic acid (1) and carbamic acid (methylene-4, 1-phenylene) bis-dimethyl ester (2) were identified from <i>A. flavus</i>. Kojic acid exhibited DPPH free radical scavenging activity with an IC<sub>50</sub> value of 99.3 μg/ml and moderate activity against ovarian teratocarcinoma (CH1), colon carcinoma (SW480), and non-small cell lung cancer (A549) cell lines. These findings suggest that endophytic fungi are able to produce promising bioactive compounds which deserve further investigation.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11294646/pdf/","citationCount":"0","resultStr":"{\"title\":\"HPLC, NMR Based Characterization, Antioxidant and Anticancer Activities of Chemical Constituents from Therapeutically Active Fungal Endophytes.\",\"authors\":\"Waqas Hussain Shah, Wajiha Khan, Sobia Nisa, Michael H J Barfuss, Johann Schinnerl, Markus Bacher, Karin Valant-Vetschera, Ashraf Ali, Hiba-Allah Nafidi, Yousef A Bin Jardan, John P Giesy\",\"doi\":\"10.4014/jmb.2403.03036\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Fungi generate different metabolites some of which are intrinsically bioactive and could therefore serve as templates for drug development. In the current study, six endophytic fungi namely <i>Aspergillus flavus</i>, <i>Aspergillus tubigenesis</i>, <i>Aspergillus oryzae</i>, <i>Penicillium oxalicum</i>, <i>Aspergillus niger</i>, and <i>Aspergillus brasiliensis</i> were isolated and identified from the medicinal plant, <i>Silybum marianum</i>. These endophytic fungi were identified through intra transcribed sequence (ITS) gene sequencing. The bioactive potentials of fungal extracts were investigated using several bioassays such as antibacterial activity by well-diffusion, MIC, MBC, anti-biofilm, antioxidant, and haemolysis. The <i>Pseudomonas aeruginosa</i> PAO1 was used to determine the antibiofilm activity. The ethyl acetate extract of <i>Aspergillus flavus</i> showed strong to moderate efficacy against <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, <i>P. aeruginosa</i>, and <i>Bacillus spizizenii</i>. <i>Aspergillus flavus</i> and <i>Aspergillus brasiliensis</i> exhibited significant antibiofilm activity with IC<sub>50</sub> at 4.02 and 3.63 mg/ml, while <i>A. flavus</i> exhibited maximum antioxidant activity of 50.8%. Based on HPLC, LC-MS, and NMR experiments kojic acid (1) and carbamic acid (methylene-4, 1-phenylene) bis-dimethyl ester (2) were identified from <i>A. flavus</i>. Kojic acid exhibited DPPH free radical scavenging activity with an IC<sub>50</sub> value of 99.3 μg/ml and moderate activity against ovarian teratocarcinoma (CH1), colon carcinoma (SW480), and non-small cell lung cancer (A549) cell lines. These findings suggest that endophytic fungi are able to produce promising bioactive compounds which deserve further investigation.</p>\",\"PeriodicalId\":16481,\"journal\":{\"name\":\"Journal of microbiology and biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-07-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11294646/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microbiology and biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.4014/jmb.2403.03036\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/6 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microbiology and biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.4014/jmb.2403.03036","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/6 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
HPLC, NMR Based Characterization, Antioxidant and Anticancer Activities of Chemical Constituents from Therapeutically Active Fungal Endophytes.
Fungi generate different metabolites some of which are intrinsically bioactive and could therefore serve as templates for drug development. In the current study, six endophytic fungi namely Aspergillus flavus, Aspergillus tubigenesis, Aspergillus oryzae, Penicillium oxalicum, Aspergillus niger, and Aspergillus brasiliensis were isolated and identified from the medicinal plant, Silybum marianum. These endophytic fungi were identified through intra transcribed sequence (ITS) gene sequencing. The bioactive potentials of fungal extracts were investigated using several bioassays such as antibacterial activity by well-diffusion, MIC, MBC, anti-biofilm, antioxidant, and haemolysis. The Pseudomonas aeruginosa PAO1 was used to determine the antibiofilm activity. The ethyl acetate extract of Aspergillus flavus showed strong to moderate efficacy against Staphylococcus aureus, Escherichia coli, P. aeruginosa, and Bacillus spizizenii. Aspergillus flavus and Aspergillus brasiliensis exhibited significant antibiofilm activity with IC50 at 4.02 and 3.63 mg/ml, while A. flavus exhibited maximum antioxidant activity of 50.8%. Based on HPLC, LC-MS, and NMR experiments kojic acid (1) and carbamic acid (methylene-4, 1-phenylene) bis-dimethyl ester (2) were identified from A. flavus. Kojic acid exhibited DPPH free radical scavenging activity with an IC50 value of 99.3 μg/ml and moderate activity against ovarian teratocarcinoma (CH1), colon carcinoma (SW480), and non-small cell lung cancer (A549) cell lines. These findings suggest that endophytic fungi are able to produce promising bioactive compounds which deserve further investigation.
期刊介绍:
The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.