具有治疗活性的真菌内生菌中化学成分的高效液相色谱和核磁共振表征、抗氧化和抗癌活性。

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of microbiology and biotechnology Pub Date : 2024-07-28 Epub Date: 2024-05-06 DOI:10.4014/jmb.2403.03036
Waqas Hussain Shah, Wajiha Khan, Sobia Nisa, Michael H J Barfuss, Johann Schinnerl, Markus Bacher, Karin Valant-Vetschera, Ashraf Ali, Hiba-Allah Nafidi, Yousef A Bin Jardan, John P Giesy
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引用次数: 0

摘要

真菌会产生不同的代谢产物,其中一些具有内在生物活性,因此可以作为药物开发的模板。在本研究中,从药用植物水飞蓟马中分离并鉴定了六种内生真菌,即黄曲霉、管曲霉、黑曲霉、草青霉、黑曲霉和巴西曲霉。这些内生真菌是通过内转录序列(ITS)基因测序确定的。使用多种生物测定方法研究了真菌提取物的生物活性潜力,如井扩散法抗菌活性、MIC、MBC、抗生物膜、抗氧化和溶血。铜绿假单胞菌菌株 PAO1 被用来测定抗生物膜活性。黄曲霉的乙酸乙酯提取物对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和芽孢杆菌有强到中等程度的抗菌效果。黄曲霉和巴西曲霉具有显著的抗生物膜活性,其 IC50 值分别为 4.02 和 3.63 毫克/毫升,而黄曲霉的抗氧化活性最高,达 50.8%。根据 HPLC、LC-MS 和 NMR 实验,从黄曲霉中鉴定出了曲酸(1)和氨基甲酸(亚甲基-4,1-亚苯)双二甲酯(2)。曲酸具有 DPPH 自由基清除活性,IC50 值为 99.3 μg/ml,对卵巢畸胎癌(CH1)、结肠癌(SW480)和非小细胞肺癌(A549)细胞株具有中等程度的活性。这些发现表明,内生真菌能够产生有前景的生物活性化合物,值得进一步研究。
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HPLC, NMR Based Characterization, Antioxidant and Anticancer Activities of Chemical Constituents from Therapeutically Active Fungal Endophytes.

Fungi generate different metabolites some of which are intrinsically bioactive and could therefore serve as templates for drug development. In the current study, six endophytic fungi namely Aspergillus flavus, Aspergillus tubigenesis, Aspergillus oryzae, Penicillium oxalicum, Aspergillus niger, and Aspergillus brasiliensis were isolated and identified from the medicinal plant, Silybum marianum. These endophytic fungi were identified through intra transcribed sequence (ITS) gene sequencing. The bioactive potentials of fungal extracts were investigated using several bioassays such as antibacterial activity by well-diffusion, MIC, MBC, anti-biofilm, antioxidant, and haemolysis. The Pseudomonas aeruginosa PAO1 was used to determine the antibiofilm activity. The ethyl acetate extract of Aspergillus flavus showed strong to moderate efficacy against Staphylococcus aureus, Escherichia coli, P. aeruginosa, and Bacillus spizizenii. Aspergillus flavus and Aspergillus brasiliensis exhibited significant antibiofilm activity with IC50 at 4.02 and 3.63 mg/ml, while A. flavus exhibited maximum antioxidant activity of 50.8%. Based on HPLC, LC-MS, and NMR experiments kojic acid (1) and carbamic acid (methylene-4, 1-phenylene) bis-dimethyl ester (2) were identified from A. flavus. Kojic acid exhibited DPPH free radical scavenging activity with an IC50 value of 99.3 μg/ml and moderate activity against ovarian teratocarcinoma (CH1), colon carcinoma (SW480), and non-small cell lung cancer (A549) cell lines. These findings suggest that endophytic fungi are able to produce promising bioactive compounds which deserve further investigation.

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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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