吡丙醚通过上调幼年激素信号，增强果蝇生殖干细胞的增殖并降低生殖能力。

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-06-12 DOI:10.1002/ps.8234

Muhammad Zaryab Khalid, Jin Liu, Jing Zhang, Liying Yang, Zhipeng Sun, Guohua Zhong

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引用次数: 0

摘要

背景：吡丙醚是一种昆虫生长调节剂（IGR），可有效防治各种害虫。然而，吡丙醚影响昆虫繁殖的分子机制仍不清楚。因此，本研究试图揭示吡丙醚对模式生物黑腹果蝇生殖系统的影响机制：结果：经吡丙醚处理后，果蝇的生殖能力明显下降。经吡丙醚处理的果蝇卵巢样本中的幼年激素（JH）滴度明显增加（120.4%）。同样，与对照组相比，吡丙醚处理组中与JH信号通路相关的关键酶的浓度和关键基因的表达量也有所增加。此外，吡丙醚处理组的生殖干细胞（GSCs）数量显著增加（15.6%），而膀胱母细胞（CBs）数量显著减少（17%）。然而，在体细胞数量方面没有观察到明显差异。我们使用生殖细胞特异性 Gal4 驱动程序对生殖细胞中与 JH 信号通路相关的五个关键基因（Met、Tai、gce、ftz-f1 和 hairy）进行了 RNA 干扰（RNAi）。有趣的是，在吡丙醚处理的转基因苍蝇中，所选基因的 RNAi 能显著减少 GSC 和 CB 的数量。这些结果进一步验证了吡丙醚通过上调JH信号增强了GSC的增殖：我们的研究结果表明，吡丙醚会影响雌性成虫卵巢中的生殖细胞，从而显著降低生殖能力。吡丙醚对生殖细胞增殖和分化的影响是由 JH 信号的增加介导的。这项研究对优化害虫控制策略、发展可持续农业实践以及了解杀虫剂的作用机制具有重要意义。© 2024 化学工业协会。

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Pyriproxyfen enhances germline stem cell proliferation and reduces reproduction in Drosophila by up-regulating juvenile hormone signaling.

Background: Pyriproxyfen is an insect growth regulator (IGR) that is effective against various types of insect pests. However, the molecular mechanism underlying pyriproxyfen effects on insect reproduction remains unclear. Thus, in this study, we attempted to uncover the mechanisms underlying the impact of pyriproxyfen on the reproductive system of the model organism Drosophila melanogaster.

Results: A significant decrease in Drosophila reproduction was observed after pyriproxyfen treatment. The juvenile hormone (JH) titer was significantly increased (120.4%) in the ovary samples of pyriproxyfen-treated flies. Likewise, the concentrations of key enzymes and the expression of key genes related to the JH signaling pathway were also increased in the pyriproxyfen-treated group compared with the control group. Furthermore, pyriproxyfen treatment significantly increased (15.6%) the number of germline stem cells (GSCs) and significantly decreased (17%) the number of cystoblasts (CBs). However, no significant differences were observed in the number of somatic cells. We performed RNA interference (RNAi) on five key genes (Met, Tai, gce, ftz-f1, and hairy) related to the JH signaling pathway in germ cells using the germ cell-specific Gal4 driver. Interestingly, RNAi of the selected genes significantly decreased the number of both GSCs and CBs in pyriproxyfen-treated transgenic flies. These results further validate that pyriproxyfen enhances GSC proliferation by up-regulating JH signaling.

Conclusion: Our results indicate that pyriproxyfen significantly decreases reproduction by affecting germ cells in female adult ovaries. The effect of pyriproxyfen on germ cell proliferation and differentiation is mediated by an increase in JH signaling. This study has significant implications for optimizing pest control strategies, developing sustainable agriculture practices, and understanding the mechanism of insecticide action. © 2024 Society of Chemical Industry.

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.