Xinyi Gong , Yan Jin , Xiao Han , Xueqi Jiang , Beibei Miao , Shuang Meng , Jingyi Zhang , Haijian Zhou , Han Zheng , Jie Feng , Juan Li
{"title":"从中国山东非孕期成人中分离出的无乳链球菌的基因组特征和耐药性特征","authors":"Xinyi Gong , Yan Jin , Xiao Han , Xueqi Jiang , Beibei Miao , Shuang Meng , Jingyi Zhang , Haijian Zhou , Han Zheng , Jie Feng , Juan Li","doi":"10.1016/j.jgar.2024.06.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><em>Streptococcus agalactiae</em> is a recognized pathogen that primarily affects infants and pregnant women. However, its increasingly important role in causing invasive infections among non-pregnant adults has become a significant health concern due to the severity and variety of its clinical impacts.</p></div><div><h3>Methods</h3><p>Nonduplicate <em>S. agalactiae</em> clinical strains associated with clinical infections (<em>n</em> = 139) were isolated from non-pregnant adults in Shandong, China. Antibiotic susceptibility testing, whole-genome sequencing and genomic analyses were conducted to characterize the genome and identify resistance features of these strains.</p></div><div><h3>Results</h3><p>The strains exhibited universal susceptibility to penicillin, ampicillin, cefotaxime, meropenem, linezolid and vancomycin. Notably, high resistance rates were observed for erythromycin (91.4%), clindamycin (89.2%), levofloxacin (84.2%), tetracycline (54.0%) and, to a lesser extent, chloramphenicol (12.9%). Serotyping revealed seven serotypes and one non-typeable strain. Serotypes Ia, Ib, III and V predominated, representing 95.7% of the strains. Nineteen sequence types were categorized into seven clonal complexes, with CC10 being the most prevalent at 48.9%. The resistance genes <em>mre</em>A (100%), <em>erm</em>B (70.5%) and <em>tet</em>M (46.0%) were commonly detected. All the isolates carried at least one pilus backbone determinant and one alpha-like protein gene, with the PI-1+PI-2a and the <em>bca</em> gene being the most frequent at 84.2% and 54.7%, respectively.</p></div><div><h3>Conclusions</h3><p>While <em>S. agalactiae</em> strains in non-pregnant adults retain sensitivity to β-lactam antibiotics, the elevated resistance to erythromycin, clindamycin, levofloxacin and tetracycline is concerning. Given the growing elderly population worldwide, the burden of <em>S. agalactiae</em> infections is significant. Continuous surveillance of serotype distribution and antibiotic resistance patterns is imperative for targeted prevention and therapeutic strategies.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages 146-153"},"PeriodicalIF":3.7000,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001115/pdfft?md5=807d63277c021014341fc48520a1ef17&pid=1-s2.0-S2213716524001115-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Genomic characterization and resistance features of Streptococcus agalactiae isolated from non-pregnant adults in Shandong, China\",\"authors\":\"Xinyi Gong , Yan Jin , Xiao Han , Xueqi Jiang , Beibei Miao , Shuang Meng , Jingyi Zhang , Haijian Zhou , Han Zheng , Jie Feng , Juan Li\",\"doi\":\"10.1016/j.jgar.2024.06.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p><em>Streptococcus agalactiae</em> is a recognized pathogen that primarily affects infants and pregnant women. However, its increasingly important role in causing invasive infections among non-pregnant adults has become a significant health concern due to the severity and variety of its clinical impacts.</p></div><div><h3>Methods</h3><p>Nonduplicate <em>S. agalactiae</em> clinical strains associated with clinical infections (<em>n</em> = 139) were isolated from non-pregnant adults in Shandong, China. Antibiotic susceptibility testing, whole-genome sequencing and genomic analyses were conducted to characterize the genome and identify resistance features of these strains.</p></div><div><h3>Results</h3><p>The strains exhibited universal susceptibility to penicillin, ampicillin, cefotaxime, meropenem, linezolid and vancomycin. Notably, high resistance rates were observed for erythromycin (91.4%), clindamycin (89.2%), levofloxacin (84.2%), tetracycline (54.0%) and, to a lesser extent, chloramphenicol (12.9%). Serotyping revealed seven serotypes and one non-typeable strain. Serotypes Ia, Ib, III and V predominated, representing 95.7% of the strains. Nineteen sequence types were categorized into seven clonal complexes, with CC10 being the most prevalent at 48.9%. The resistance genes <em>mre</em>A (100%), <em>erm</em>B (70.5%) and <em>tet</em>M (46.0%) were commonly detected. All the isolates carried at least one pilus backbone determinant and one alpha-like protein gene, with the PI-1+PI-2a and the <em>bca</em> gene being the most frequent at 84.2% and 54.7%, respectively.</p></div><div><h3>Conclusions</h3><p>While <em>S. agalactiae</em> strains in non-pregnant adults retain sensitivity to β-lactam antibiotics, the elevated resistance to erythromycin, clindamycin, levofloxacin and tetracycline is concerning. Given the growing elderly population worldwide, the burden of <em>S. agalactiae</em> infections is significant. 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Genomic characterization and resistance features of Streptococcus agalactiae isolated from non-pregnant adults in Shandong, China
Background
Streptococcus agalactiae is a recognized pathogen that primarily affects infants and pregnant women. However, its increasingly important role in causing invasive infections among non-pregnant adults has become a significant health concern due to the severity and variety of its clinical impacts.
Methods
Nonduplicate S. agalactiae clinical strains associated with clinical infections (n = 139) were isolated from non-pregnant adults in Shandong, China. Antibiotic susceptibility testing, whole-genome sequencing and genomic analyses were conducted to characterize the genome and identify resistance features of these strains.
Results
The strains exhibited universal susceptibility to penicillin, ampicillin, cefotaxime, meropenem, linezolid and vancomycin. Notably, high resistance rates were observed for erythromycin (91.4%), clindamycin (89.2%), levofloxacin (84.2%), tetracycline (54.0%) and, to a lesser extent, chloramphenicol (12.9%). Serotyping revealed seven serotypes and one non-typeable strain. Serotypes Ia, Ib, III and V predominated, representing 95.7% of the strains. Nineteen sequence types were categorized into seven clonal complexes, with CC10 being the most prevalent at 48.9%. The resistance genes mreA (100%), ermB (70.5%) and tetM (46.0%) were commonly detected. All the isolates carried at least one pilus backbone determinant and one alpha-like protein gene, with the PI-1+PI-2a and the bca gene being the most frequent at 84.2% and 54.7%, respectively.
Conclusions
While S. agalactiae strains in non-pregnant adults retain sensitivity to β-lactam antibiotics, the elevated resistance to erythromycin, clindamycin, levofloxacin and tetracycline is concerning. Given the growing elderly population worldwide, the burden of S. agalactiae infections is significant. Continuous surveillance of serotype distribution and antibiotic resistance patterns is imperative for targeted prevention and therapeutic strategies.
期刊介绍：
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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