免疫刺激核酸纳米颗粒(NANPs)可增强保护性成骨细胞和破骨细胞对金黄色葡萄球菌的 I 型干扰素反应。

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-10 DOI:10.1016/j.nano.2024.102762
Erin L. Mills PhD , Yelixza I. Avila PhD , Damian Beasock PhD , Yasmine Radwan PhD , Samantha R. Suptela PhD , Ian Marriott PhD , Kirill A. Afonin PhD , M. Brittany Johnson PhD
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引用次数: 0

摘要

顽固性葡萄球菌骨髓炎的部分原因可能是金黄色葡萄球菌能够侵入骨细胞。然而,现在人们认识到破骨细胞和成骨细胞能影响宿主对细菌感染的反应,我们最近描述了它们在金黄色葡萄球菌感染后产生 IFN-β 并限制细胞内细菌存活/繁殖的能力。在这里,我们研究了合理设计的新型核酸纳米颗粒(NANPs)在导入骨细胞后诱导产生免疫介质(包括 IFN-β)的能力。我们展示了具有代表性的 NANPs 与脂质载体复合后,通过内泌体转运成功地输送到成骨细胞和破骨细胞中。我们发现,根据纳米蛋白的组成和结构,它们可以通过离散的细胞膜模式识别受体诱导不同的免疫反应。最后,免疫刺激纳米粒子能增强受金葡菌感染的骨细胞产生 IFN-β,并减少细胞内细菌负担,这证明了这种纳米粒子技术的实用性。
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Immunostimulatory nucleic acid nanoparticles (NANPs) augment protective osteoblast and osteoclast type I interferon responses to Staphylococcus aureus

Recalcitrant staphylococcal osteomyelitis may be due, in part, to the ability of Staphylococcus aureus to invade bone cells. However, osteoclasts and osteoblasts are now recognized to shape host responses to bacterial infection and we have recently described their ability to produce IFN-β following S. aureus infection and limit intracellular bacterial survival/propagation. Here, we have investigated the ability of novel, rationally designed, nucleic acid nanoparticles (NANPs) to induce the production of immune mediators, including IFN-β, following introduction into bone cells. We demonstrate the successful delivery of representative NANPs into osteoblasts and osteoclasts via endosomal trafficking when complexed with lipid-based carriers. Their delivery was found to differentially induce immune responses according to their composition and architecture via discrete cytosolic pattern recognition receptors. Finally, the utility of this nanoparticle technology was supported by the demonstration that immunostimulatory NANPs augment IFN-β production by S. aureus infected bone cells and reduce intracellular bacterial burden.

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来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
期刊最新文献
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