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引用次数: 0
摘要
微生物生态学家使用阿尔法多样性指标来估算小亚基核糖体 RNA 基因高通量测序数据中的物种丰富度和均匀度。这一观点认为,非参数富集度估算器 Chao1 和 ACE 不应与 ASV 数据一起使用,因为生成扩增子序列变体(ASV)的默认过程会去除单子,而单子是这些估算计算所特别需要的。此外,在可能的情况下保留单子,也会给这一丰度类别带来很大比例的假象,导致丰富度被极度高估。我们建议使用其他序列聚类策略和/或多样性指标,以避免 ASV 数据产生毫无意义的丰富度估计值。
期刊介绍:
The ISME Journal covers the diverse and integrated areas of microbial ecology. We encourage contributions that represent major advances for the study of microbial ecosystems, communities, and interactions of microorganisms in the environment. Articles in The ISME Journal describe pioneering discoveries of wide appeal that enhance our understanding of functional and mechanistic relationships among microorganisms, their communities, and their habitats.