Francesca Laneri, Cristina Parisi, Mimimorena Seggio, Aurore Fraix, Giuseppe Longobardi, Ovidio Catanzano, Fabiana Quaglia and Salvatore Sortino
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引用次数: 0
摘要
一氧化氮(NO)的生物效应与其浓度和生成部位密切相关,这就要求对这种无机自由基的传递进行精确的时空控制。用合适的一氧化氮光前驱体进行光激活是一种理想的方法。利用治疗窗口(650-1300 纳米)内的长波长激发光来激活 NO 释放的策略开发具有挑战性。在这篇论文中,我们证明了具有自发荧光报告功能的蓝光可激活 NO 光引发剂(NOPD)可利用超分子光敏化过程,通过生物相容性更好的红光催化 NO 释放。不同的红光吸收光敏剂(PSs)与 NOPD 共同包裹在不同的生物相容性纳米载体(如 Pluronic® 胶束、微乳液和支链环糊精聚合物)中。载体内光敏 NO 的释放涉及作为关键中间体的 PS 的最低长寿命三重态以及 NOPD 对其的淬灭,与分子氧的释放具有竞争性。因此,即使在有氧条件下,NO 的释放效果也很好。因此,所采用的一般策略为利用已有的 NOPD 生成氮氧化物提供了一种宝贵的工具,这种 NOPD 可通过生物相容性较差的蓝光激活,而无需进行任何化学修饰,也无需使用复杂而昂贵的辐照源。
Supramolecular red-light-photosensitized nitric oxide release with fluorescence self-reporting within biocompatible nanocarriers
The strict dependence of the biological effects of nitric oxide (NO) on its concentration and generation site requires this inorganic free radical to be delivered with precise spatiotemporal control. Light-activation by suitable NO photoprecursors represents an ideal approach. Developing strategies to activate NO release using long-wavelength excitation light in the therapeutic window (650–1300 nm) is challenging. In this contribution, we demonstrate that NO release by a blue-light activatable NO photodonor (NOPD) with self-fluorescence reporting can be triggered catalytically by the much more biocompatible red light exploiting a supramolecular photosensitization process. Different red-light absorbing photosensitizers (PSs) are co-entrapped with the NOPD within different biocompatible nanocarriers such as Pluronic® micelles, microemulsions and branched cyclodextrin polymers. The intra-carrier photosensitized NO release, involving the lowest, long-lived triplet state of the PS as the key intermediate and its quenching by the NOPD, is competitive with that by molecular oxygen. This allows NO to be released with good efficacy, even under aerobic conditions. Therefore, the adopted general strategy provides a valuable tool for generating NO from an already available NOPD, otherwise activatable with the poorly biocompatible blue light, without requiring any chemical modification and using sophisticated and expensive irradiation sources.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices