J. Soul , E. Carlsson , S.R. Hofmann , S. Russ , J. Hawkes , F. Schulze , M. Sergon , J. Pablik , S. Abraham , C.M. Hedrich
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引用次数: 0
摘要
临床和病理机制特征的重叠会使包括银屑病和特应性皮炎(AD)在内的炎症性皮肤病的诊断和治疗复杂化。空间转录组学可以识别疾病和细胞特异性分子特征,从而推动生物标记物的开发和未来的治疗。这项研究确定了银屑病和 AD 患者的角朊细胞和基底下 CD4+ 和 CD8+ T 淋巴细胞的转录特征。对配体与受体之间的相互作用进行硅学预测,提供关键信号通路(干扰素、效应 T 细胞、基质细胞和基质生物学、神经元发育等)。在外周血 T 细胞中对包括 CCL22、RELB 和 JUND 在内的选定转录本进行的靶向验证表明,所选方法在其他炎症性疾病中也是一种很有前途的工具。牛皮癣和注意力缺失症的特点是 T 细胞和角质形成细胞的转录失调,这些都可能成为治疗的目标。空间转录组学是寻找可用作生物标记和/或治疗目标的分子特征的重要工具。
Tissue gene expression profiles and communication networks inform candidate blood biomarker identification in psoriasis and atopic dermatitis
Overlapping clinical and pathomechanistic features can complicate the diagnosis and treatment of inflammatory skin diseases, including psoriasis and atopic dermatitis (AD). Spatial transcriptomics allows the identification of disease- and cell-specific molecular signatures that may advance biomarker development and future treatments.
This study identified transcriptional signatures in keratinocytes and sub-basal CD4+ and CD8+ T lymphocytes from patients with psoriasis and AD. In silico prediction of ligand:receptor interactions delivered key signalling pathways (interferon, effector T cells, stroma cell and matrix biology, neuronal development, etc.). Targeted validation of selected transcripts, including CCL22, RELB, and JUND, in peripheral blood T cells suggests the chosen approach as a promising tool also in other inflammatory diseases.
Psoriasis and AD are characterized by transcriptional dysregulation in T cells and keratinocytes that may be targeted therapeutically. Spatial transcriptomics is a valuable tool in the search for molecular signatures that can be used as biomarkers and/or therapeutic targets.
期刊介绍:
Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.