脑一氧化碳可通过脑γ-氨基丁酸受体抑制大鼠的排尿反射。

IF 1.8 3区医学 Q3 UROLOGY & NEPHROLOGY International Journal of Urology Pub Date : 2024-06-17 DOI:10.1111/iju.15514

Masaki Yamamoto, Takahiro Shimizu, Nobutaka Shimizu, Mikiya Fujieda, Motoaki Saito

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引用次数: 0

摘要

目的：研究内源性气体递质一氧化碳（CO）在调节大鼠排尿反射中的作用：研究脑内一氧化碳（CO）（一种内源性气体递质）在调节大鼠排尿反射中的作用：方法：在对雄性大鼠进行尿烷麻醉（0.8 克/千克，ip）后，在脑室内注射 CORM-3（CO 供体）或 ZnPP（血红素加氧酶（一种 CO 生成酶）的非选择性抑制剂）前 1 小时开始评估尿动力学参数，并在注射后持续 2 小时。我们还研究了中枢预处理 SR95531（GABAA 受体拮抗剂）或 SCH50911（GABAB 受体拮抗剂）对 CORM-3 诱导反应的影响：结果：CORM-3能明显延长收缩间期（ICIs），而不改变最大排尿压（MVP）；ZnPP能明显缩短ICI，减少单次排尿量和膀胱容量，而不影响MVP、排尿后残余量或排尿效率。CORM-3 可逆转 ZnPP 诱导的 ICI 缩短。CORM-3诱导的ICI延长分别被中枢预处理的SR95531或SCH50911显著减弱：结论：脑CO可通过脑γ-氨基丁酸（GABA）受体抑制大鼠的排尿反射。

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Brain carbon monoxide can suppress the rat micturition reflex through brain γ-aminobutyric acid receptors

Objectives

To investigate roles of brain carbon monoxide (CO), an endogenous gasotransmitter, in regulation of the rat micturition reflex.

Methods

In urethane-anesthetized (0.8 g/kg, ip) male rats, evaluation of urodynamic parameters was started 1 h before intracerebroventricular administration of CORM-3 (CO donor) or ZnPP (non-selective inhibitor of heme oxygenase, a CO producing enzyme) and continued for 2 h after the administration. We also investigated effects of centrally pretreated SR95531 (GABA_A receptor antagonist) or SCH50911 (GABA_B receptor antagonist) on the CORM-3-induced response.

Results

CORM-3 significantly prolonged intercontraction intervals (ICIs) without changing maximal voiding pressure (MVP), while ZnPP significantly shortened ICI and reduced single-voided volume and bladder capacity without affecting MVP, post-voided residual volume, or voiding efficiency. The ZnPP-induced ICI shortening was reversed by CORM-3. The CORM-3-induced ICI prolongation was significantly attenuated by centrally pretreated SR95531 or SCH50911, respectively.

Conclusions

Brain CO can suppress the rat micturition reflex through brain γ-aminobutyric acid (GABA) receptors.

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来源期刊

International Journal of Urology 医学-泌尿学与肾脏学

CiteScore

4.70

自引率

11.50%

发文量

340

审稿时长

3 months

期刊介绍： International Journal of Urology is the official English language journal of the Japanese Urological Association, publishing articles of scientific excellence in urology. Submissions of papers from all countries are considered for publication. All manuscripts are subject to peer review and are judged on the basis of their contribution of original data and ideas or interpretation.