Adriana C da Silva, Jadel M Kratz, Priscylla G M Morgado, Lucio H Freitas-Junior, Carolina B Moraes
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引用次数: 0
摘要
为了控制和减少恰加斯病、利什曼病和非洲人锥虫病等人类原生动物疾病对公共卫生的影响,有必要加快新药和疫苗的开发。然而,这一过程困难重重,例如寄生虫生物学和疾病发病机理非常复杂,而且作为被忽视的热带疾病的典型特征,用于研究和开发的资金相对有限。因此,既能充分再现感染和疾病的主要特征,又能合理利用资源的体外和体内研究模型对于推进这些疾病的研究至关重要。恰加斯病的体内生物发光成像(BLI)小鼠模型就是一个例子,该模型可对表达荧光素酶的克鲁斯锥虫寄生虫产生的长波长光进行高灵敏度检测。尽管该技术已成为体内药效研究的标准方法,但由于缺乏有关设备处理和质量控制程序应用的适当实践培训,即使有现成的合适 BLI 设备,研究小组仍可能难以实施该技术。考虑到这种情况,本方案旨在指导从规划实验到数据采集和分析的整个过程,并提供一些细节,以便于对恰加斯病或其他传染病小鼠模型缺乏或没有 BLI 经验的研究小组实施方案。
Demystifying In Vivo Bioluminescence Imaging of a Chagas Disease Mouse Model for Drug Efficacy Studies.
To control and decrease the public health impact of human protozoan diseases such as Chagas disease, leishmaniasis, and human African trypanosomiasis, expediting the development of new drugs and vaccines is necessary. However, this process is filled with difficulties such as highly complex parasite biology and disease pathogenesis and, as typical for neglected tropical diseases, comparatively limited funding for research and development. Thus, in vitro and in vivo study models that can sufficiently reproduce infection and disease key features while providing rational use of resources are essential for progressing research for these conditions. One example is the in vivo bioluminescence imaging (BLI) mouse model for Chagas disease, which provides highly sensitive detection of long wavelength light generated by Trypanosoma cruzi parasites expressing luciferase. Despite this technique becoming the standard approach for drug efficacy in vivo studies, research groups might still struggle to implement it due to a lack of proper practical training on equipment handling and application of quality control procedures, even when suitable BLI equipment is readily available. Considering this scenario, this protocol aims to guide from planning experiments to data acquisition and analysis, with details that facilitate the implementation of protocols in research groups with little or no experience with BLI, either for Chagas disease or for other infectious disease mouse models.
期刊介绍:
JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.