西龙目岛克迪里地区产蛋鸡新鲜粪便中沙门氏菌的抗生素耐药性模式

Fitrah Akbar Ramdani, Katty Hendriana, Priscilia Riwu, Kholik Kholik, Yonanda Verawati, Haslinda Mbura, Herdin Vanek Zakarias
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摘要

抗生素耐药性已成为印度尼西亚家禽养殖业的一个常见问题。其中一个原因是抗生素使用不当。本研究旨在确定从蛋鸡中分离出的沙门氏菌对抗生素的耐药性模式。本研究使用了 30 份来自西龙目岛克迪里区的蛋鸡新鲜粪便样本。通过革兰氏染色和生化测试(吲哚、甲基红-Voges Proskauer、三糖铁琼脂、柠檬酸盐和尿素酶)对样本进行分离和鉴定,结果发现 10 个沙门氏菌阳性样本。 研究继续使用盘扩散法或柯比鲍尔法进行抗生素耐药性测试。研究中使用的抗生素有阿曲南安、环丙沙星、链霉素、氯霉素和四环素。结果显示,沙门氏菌对一些抗生素产生了耐药性。耐药率最高的是链霉素和四环素,分别为 50%(5/10),最低的是环丙沙星,为 10%(1/10)。仍然敏感的抗生素是阿曲南为 90%(9/10),氯霉素为 100%(10/10)。这项研究的结论是,蛋鸡养殖场存在抗生素耐药性模式,可能对人类和动物健康产生负面影响。
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Pattern of Antibiotic Resistance in Salmonella sp. Bacteria Contaminating Fresh Faeces of Laying Hens in Kediri District, West Lombok Regency
Antibiotic resistance has become a common problem in poultry farming in Indonesia. One of them is caused by improper use of antibiotics. This study aims to determine the pattern of resistance of Salmonella sp. isolated from laying hens to antibiotics. The samples used in this study were thirty samples of fresh faeces of laying hens from Kediri District, West Lombok Regency. The samples were isolated and identified through Gram staining and biochemical tests (indole, methyl red-Voges Proskauer, triple sugar iron agar, citrate, and urease), resulting in ten positive samples of Salmonella sp. The study continued with antibiotic resistance testing using disc diffusion or Kirby-Bauer methods. The antibiotics used in the study were aztreonam, ciprofloxacin, streptomycin, chloramphenicol, and tetracycline. The results showed that Salmonella sp. has been resistant to some antibiotics. The highest resistance levels are streptomycin and tetracycline at 50% (5/10), while the lowest is ciprofloxacin at 10% (1/10). Antibiotics that are still sensitive are aztreonam 90% (9/10) and chloramphenicol 100% (10/10). The findings of this study conclude that there is a pattern of antibiotic resistance in laying hens farms that can have a negative impact on human and animal health.
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