利用 DNA 标记研究夏洛莱肉牛群体的基因多态性

IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Agricultural Science and Practice Pub Date : 2024-06-13 DOI:10.15407/agrisp11.01.014
R. O. Kulibaba, Yurii Liashenko, S. V. Kulibaba, M. Sakhatskyi
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BclI-polymorphism of the first exon was used to determine the excess of heterozygous animals (81.8 %);\nthere were no animals homozygous by allele A. By polymorphism of locus SLC11A1 for SNP6 (7808A>T), we deter-\nmined complete domination of the frequency of allele A over T (0.985 vs 0.015), no animals, homozygous by allele\nTT, were found. As for SNP5 7400C>G, there were no animals homozygous for allele G; the ratio of alleles C and G\nwas 0.79 and 0.21, respectively. By locus IFNGR2 (1008A>G), we determined the prevalence of the frequency of al-\nlele A over G (0.745 vs 0.255); the animals with genotype AA were the most common. As for gene TNFα (–824A>G),\nwe determined a two-fold prevalence of the frequency of allele G over A due to a higher number of animals with geno-\ntype GG (39 %). Some 50 % of the animals were heterozygous for this gene.By locus MBL1,we found the prevalence\nof the frequency of allele G (StyI-) as compared to A (StyI+) due to a considerable number of homozygous animals\nGG (64 %). The percentage of homozygous animals was 37.8 %. Conclusions. General characteristics of genetic\nvariability of the Charolais beef cattle population were determined for the loci TLR1, TLR4, IFNGR2, SLC11A1,\nTNFα, and MBL1. As for SNP6 7808A>T of locus SLC11A1, we have determined the final stage of allele A fixation,\nby the results of which the gene will become completely monomorphic by this mutation. The results of the analysis\nof the ratio between the frequencies of alleles and genotypes by the revealed polymorphic markers demonstrated that\nit is unreasonable to use animals with different allelic variants of genes TLR1, IFNGR2, SLC11A1 in further studies\non the search for associations with the manifestation of economically valuable traits in Charolais beef cows of the\nexperimental population due to insufficient number of animals with different genotypes. For loci TNFα and MBL1, we\nfound enough animals with different genotypes to conduct further association studies. 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引用次数: 0

摘要

目的研究夏洛莱肉牛群体中与不同疾病抵抗力相关的基因(TLR1、TLR4、SLC11A1、TLR1、TLR4、IFNGR2、SLC11A1、TNFα 和 MBL1)的多态性。研究方法采用聚合酶链式反应和限制性片段长度多态性(PCR-RFLP)方法进行研究;采用人工创建限制性位点-PCR(ACRS-PCR)方法研究MBL1基因的多态性。结果在夏洛莱牛群体(n = 100)中,基因 TLR1、IFNGR2、SLC11A1、TNFα 和 MBL1 呈多态性,而 TLR4 则由三个突变变体(8732G>A、8834G>C 和 2021C>T)形成单态性。通过 SLC11A1 基因座 SNP6(7808A>T)的多态性,我们发现等位基因 A 的频率完全高于 T(0.985 对 0.015),没有发现等位基因TT 的动物。至于 SNP5 7400C>G,没有等位基因 G 的同源动物;等位基因 C 和 Gw 的比例分别为 0.79 和 0.21。通过基因位点 IFNGR2(1008A>G),我们确定了等位基因 A 的频率高于等位基因 G 的频率(0.745 vs 0.255);基因型为 AA 的动物最为常见。至于 TNFα 基因(-824A>G),由于基因型为 GG 的动物数量较多(39%),我们确定等位基因 G 的频率是 A 的两倍。通过基因位点 MBL1,我们发现等位基因 G(StyI-)的频率是 A(StyI+)的两倍,原因是相当多的动物为 GG 基因型(64%)。等位基因 G 的比例为 37.8%。结论确定了夏洛莱肉牛群体中 TLR1、TLR4、IFNGR2、SLC11A1、TNFα 和 MBL1 基因位点遗传可变性的一般特征。对于 SLC11A1 基因座的 SNP6 7808A>T,我们确定了等位基因 A 固定化的最后阶段,根据该阶段的结果,该基因将因该突变而完全单态化。通过所揭示的多态性标记对等位基因频率和基因型频率之间的比率进行分析的结果表明,由于具有不同基因型的动物数量不足,使用基因 TLR1、IFNGR2 和 SLC11A1 的不同等位基因变异动物来进一步研究夏洛莱肉牛经济价值性状表现的相关性是不合理的。对于基因位点 TNFα 和 MBL1,我们找到了足够多的不同基因型的动物来进行进一步的关联研究。在人群中观察到 TLR1、SLC11A1 (7400C>G) 和 MBL1 基因位点偏离遗传哈代-温伯格平衡。
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Gene polymorphism in a population of Charolais beef cattle using DNA-markers
Aim. To study the polymorphism of genes, associated with resistance to different diseases (TLR1, TLR4, SLC11A1, TLR1, TLR4, IFNGR2, SLC11A1, TNFα, and MBL1) in the population of Charolais beef cattle. Methods. The studies were conducted using the methods of polymerase chain reaction and the restriction fragment length polymorphism (PCR-RFLP); the artificially created restriction site-PCR (ACRS-PCR) was used to study the polymorphism of gene MBL1. Results. In the population (n = 100) of Charolais cattle, genes TLR1, IFNGR2, SLC11A1, TNFα, and MBL1 were polymorphic, while TLR4 was found to be monomorphic by three mutant variants (8732G>A, 8834G>C, and 2021C>T). BclI-polymorphism of the first exon was used to determine the excess of heterozygous animals (81.8 %); there were no animals homozygous by allele A. By polymorphism of locus SLC11A1 for SNP6 (7808A>T), we deter- mined complete domination of the frequency of allele A over T (0.985 vs 0.015), no animals, homozygous by allele TT, were found. As for SNP5 7400C>G, there were no animals homozygous for allele G; the ratio of alleles C and G was 0.79 and 0.21, respectively. By locus IFNGR2 (1008A>G), we determined the prevalence of the frequency of al- lele A over G (0.745 vs 0.255); the animals with genotype AA were the most common. As for gene TNFα (–824A>G), we determined a two-fold prevalence of the frequency of allele G over A due to a higher number of animals with geno- type GG (39 %). Some 50 % of the animals were heterozygous for this gene.By locus MBL1,we found the prevalence of the frequency of allele G (StyI-) as compared to A (StyI+) due to a considerable number of homozygous animals GG (64 %). The percentage of homozygous animals was 37.8 %. Conclusions. General characteristics of genetic variability of the Charolais beef cattle population were determined for the loci TLR1, TLR4, IFNGR2, SLC11A1, TNFα, and MBL1. As for SNP6 7808A>T of locus SLC11A1, we have determined the final stage of allele A fixation, by the results of which the gene will become completely monomorphic by this mutation. The results of the analysis of the ratio between the frequencies of alleles and genotypes by the revealed polymorphic markers demonstrated that it is unreasonable to use animals with different allelic variants of genes TLR1, IFNGR2, SLC11A1 in further studies on the search for associations with the manifestation of economically valuable traits in Charolais beef cows of the experimental population due to insufficient number of animals with different genotypes. For loci TNFα and MBL1, we found enough animals with different genotypes to conduct further association studies. The deviation from the genetic Hardy-Weinberg equilibrium was observed for the loci TLR1, SLC11A1 (7400C>G), and MBL1 in the population.
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Agricultural Science and Practice
Agricultural Science and Practice AGRICULTURE, MULTIDISCIPLINARY-
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