全面分析病原体感染期间柑橘中与活性氧代谢有关的基因

Guiyan Huang, Fuxuan Li, Yanan Hu, Zhigang Ouyang, Ruimin Li
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引用次数: 0

摘要

活性氧(ROS)在植物与病原体相互作用的信号转导过程中起着关键作用。目前还不清楚参与柑橘(甜橙)中亚洲自由杆菌(CLas)和柠檬黄单胞菌亚种(Xcc)感染的 ROS 信号传导途径。在这项研究中,我们全面鉴定了与 ROS 代谢相关的基因,包括 9 个 NADPH 氧化酶(RBOH)、14 个超氧化物歧化酶(SOD)、1 个过氧化氢酶(CAT)、9 个过氧化还原酶(PrxR)、5 个抗坏血酸过氧化物酶(APX)、4 个谷胱甘肽过氧化物酶(APX)、1 个过氧化氢酶(CAT)和 1 个过氧化氢酶(CAT)、4 个谷胱甘肽过氧化物酶 (GPX)、3 个单脱氢抗坏血酸还原酶 (MDAR)、2 个脱氢抗坏血酸还原酶 (DHAR)、2 个谷胱甘肽还原酶 (GR)、24 个硫代氧化还原酶 (Trx) 和 18 个谷胱甘肽还原酶 (GLR) 基因。中的基因。分析表明,在 ROS 亚家族中,基因结构各不相同,但基序和结构域是一致的。与拟南芥和葡萄的同源比较分析表明,大多数与 ROS 代谢相关的基因在进化过程中保持不变,其中一些基因来源于 C. sinensis 中物种分化后的基因复制事件。表达谱分析显示,在CLas和Xcc感染期间,有5个基因上调,4个基因下调。启动子分析表明,ROS代谢相关基因的启动子中有许多应激反应元件。蛋白质-蛋白质相互作用网络分析强调了 ROS 代谢在各种生物过程中的参与。对中华皂角树和三叶皂角树的 ROS 代谢相关基因进行比较后发现,中华皂角树 ROS 亚家族中存在多个基因增益和缺失事件。这项研究加深了我们对中华猕猴桃 ROS 代谢的了解,并揭示了柑橘与病原体之间的相互作用。
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Comprehensive Analysis of Genes Associated with the Reactive Oxygen Species Metabolism in Citrus sinensis during Pathogen Infection
Reactive oxygen species (ROS) are pivotal in signal transduction processes in plant–pathogen interactions. The ROS signaling pathways involved in Candidatus Liberibacter asiaticus (CLas) and Xanthomonas citri subspecies citri (Xcc) infections in Citrus sinensis (sweet orange) are unclear. In this study, we comprehensively identified ROS metabolism-associated genes, including 9 NADPH oxidase (RBOH), 14 superoxide dismutase (SOD), 1 catalase (CAT), 9 peroxiredoxin (PrxR), 5 ascorbate peroxidase (APX), 4 glutathione peroxidase (GPX), 3 monodehydroascorbate reductase (MDAR), 2 dehydroascorbate reductase (DHAR), 2 glutathione reductase (GR), 24 thioredoxin (Trx), and 18 glutaredoxin (GLR) genes in C. sinensis. An analysis revealed variable gene structures but conserved motifs and domains in ROS subfamilies. A comparative synteny analysis with Arabidopsis thaliana and Vitis vinifera indicated evolutionary conservation of most ROS metabolism-associated genes, with some originating from gene duplication events post-species divergence in C. sinensis. Expression profiling revealed five up-regulated genes and four down-regulated genes during both CLas and Xcc infections. Promoter analysis revealed numerous stress-responsive elements in the promoter of ROS metabolism-associated genes. Protein–protein interaction network analysis highlighted the involvement of ROS metabolism in various biological processes. A comparison of ROS metabolism-associated genes between C. sinensis and Poncirus trifoliata indicated multiple gene gain and loss events within ROS subfamilies of C. sinensis. This study enhances our understanding of ROS metabolism in C. sinensis and sheds light on citrus–pathogen interactions.
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