以木糖为主要碳源,通过浸没发酵法寻找木糖利用率高、能产生外多糖 (EPS) 的细菌并进行 EPS 定量

U. Antia, Nsikak Stephen, Victoria Umoh, Maria Bassey, Itorobong Udo, A. J. Adeleke
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Adeleke","doi":"10.47430/ujmr.2493.003","DOIUrl":null,"url":null,"abstract":"Study’s Novelty/ Excerpt\n\nThis study identified bacterial isolates from diverse environments capable of utilizing xylose for exo-polysaccharide (EPS) production, an area with limited prior research.\nPotential EPS producers were isolated and identified as Staphylococcus, Enterobacter, Kocuria, Klebsiella, Enterococcus, Serratia, and Burkholderia species using a salt-based medium with xylose as the primary carbon source. \nThe highest EPS production was observed in Enterobacter cloacae D1, E. cloacae D2, Klebsiella oxytoca D2, and K. oxytoca G1, ranging from 0.04 g/L to 2.0 g/L, highlighting these isolates' potential for optimization and application in various industries.\n\nFull Abstract\nMany microorganisms are capable of producing Exo-polysaccharides (EPS) while utilizing simple sugars and hexoses. 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引用次数: 0

摘要

研究的新颖性/摘要本研究从不同环境中鉴定出了能够利用木糖生产外多糖(EPS)的细菌分离物,而这一领域之前的研究还很有限。使用以木糖为主要碳源的盐基培养基,分离并鉴定出了潜在的 EPS 生产者,包括葡萄球菌、肠杆菌、克雷伯氏菌、肠球菌、沙雷氏菌和伯克霍尔德氏菌。肠杆菌 D1、E. cloacae D2、克雷伯氏菌 D2 和 K. oxytoca G1 的 EPS 产量最高,从 0.04 克/升到 2.0 克/升不等,凸显了这些分离物在各行业中的优化和应用潜力。这些 EPS 可应用于农业生物技术、制药、纺织和食品工业等多个领域。然而,目前还缺乏对能利用木糖等戊糖的 EPS 合成细菌的研究。因此,利用替代碳源生产 EPS 已成为近期研究的重点。本研究旨在探寻可利用木糖生产 EPS 的细菌。研究人员将来自农业土壤、垃圾场、盐碱地、水泥污染土壤、新鲜牛乳、牛粪和酸奶的样本进行连续稀释,并在以木糖为主要碳源的盐基培养基中进行培养。粘液状和黏液状菌落被选为可能产生 EPS 的分离物,并使用 VITEK 2 自动识别系统进行形态和生化鉴定。以木糖为唯一碳源,通过浸没发酵对这些分离菌的 EPS 产量进行量化。木糖利用细菌的平均异养菌计数范围为每克样品 2.1x106 CFU 至 3.5x108 CFU。经鉴定,粘液和粘液菌落属于葡萄球菌属、肠杆菌属、高库氏菌属、克雷伯氏菌属、肠球菌属、沙雷氏菌属和伯克霍尔德氏菌属。分离菌株产生的 EPS 量从 0.04 克/升到 2.0 克/升不等,其中 E. cloacae D1、E. cloacae D2、K. oxytoca D2 和 K. oxytoca G1 产生的 EPS 量最高。能利用木糖生产 EPS 的细菌分离物来自不同来源,显示出进一步优化的潜力
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Bio-Prospecting Xylose-Utilizing, Exopolysaccharide (EPS)-Producing Bacteria and EPS Quantification through Submerged Fermentation using Xylose as the Major Carbon Source
Study’s Novelty/ Excerpt This study identified bacterial isolates from diverse environments capable of utilizing xylose for exo-polysaccharide (EPS) production, an area with limited prior research. Potential EPS producers were isolated and identified as Staphylococcus, Enterobacter, Kocuria, Klebsiella, Enterococcus, Serratia, and Burkholderia species using a salt-based medium with xylose as the primary carbon source. The highest EPS production was observed in Enterobacter cloacae D1, E. cloacae D2, Klebsiella oxytoca D2, and K. oxytoca G1, ranging from 0.04 g/L to 2.0 g/L, highlighting these isolates' potential for optimization and application in various industries. Full Abstract Many microorganisms are capable of producing Exo-polysaccharides (EPS) while utilizing simple sugars and hexoses. These EPS found applications in various fields, such as agricultural biotechnology, pharmaceuticals, textiles, and food industries. However, there is a lack of studies on EPS-elaborating bacteria that can utilize pentoses like xylose. Therefore, the utilization of alternative carbon sources for EPS production has become a focus of recent research. This study aimed to prospect bacteria that can utilize xylose for EPS production. Samples from agricultural soil, dump sites, saline soil, cement-contaminated soil, fresh cow milk, cow dung, and yogurt were serially diluted and cultured in a salt-based medium with xylose as the primary carbon source. Slimy and mucoid colonies were selected as potential EPS-producing isolates and identified morphologically and biochemically using the VITEK 2 Automated identification system. The quantification of EPS production by these isolates was conducted through submerged fermentation with xylose as the sole carbon source. The mean heterotrophic bacterial count of xylose-utilizing bacteria ranged from 2.1x106 CFU to 3.5x108 CFU per gram of the samples analyzed. The slimy and mucoid colonies were identified as members of the genera Staphylococcus, Enterobacter, Kocuria, Klebsiella, Enterococcus, Serratia, and Burkholderia. The quantities of EPS produced by the isolates ranged from 0.04 g/L to 2.0 g/L, with E. cloacae D1, E. cloacae D2, K. oxytoca D2, and K. oxytoca G1 elaborating the highest amount of EPS. Bacterial isolates capable of utilizing xylose for EPS production were obtained from various sources, showing potential for further optimization
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Molecular Characterization of Agents of Cutaneous Leishmaniasis in Dutsin-Ma Local Government Area of Katsina State, Northwestern, Nigeria Bio-Prospecting Xylose-Utilizing, Exopolysaccharide (EPS)-Producing Bacteria and EPS Quantification through Submerged Fermentation using Xylose as the Major Carbon Source Optimization of Growth Response Parameters, Screening and Molecular Detection of Pesticide Degradation Genes in Bacterial Isolates from Agricultural Soils Comparative Effect of Breast Milk and Infant Formulae on Neonatal Gut Microbiome within Katsina Metropolis Occurrence of Antibiotic Resistant Salmonella and Shigella in Diarrheal cases Resulting from a Common Source Consumption of Contaminated Water
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