检测坏死杆菌亚种对泰乐菌素的抗药性

IF 2.3 Q1 AGRICULTURE, MULTIDISCIPLINARY ACS agricultural science & technology Pub Date : 2024-06-07 DOI:10.1021/acsagscitech.4c00159
Cory Schwarz, Jacques Mathieu*, Jenny Laverde Gomez, Marina Tikhonova, T. G. Nagaraja and Pedro J. J. Alvarez, 
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摘要

饲用泰乐菌素是一种大环内酯类药物,通过抑制瘤胃坏死镰刀菌的生长,被广泛用于预防饲养场牛的肝脓肿。尽管泰乐菌素已使用了近五十年,但从未分离出耐药的坏死杆菌亚种菌株。在此,我们报告了先前从脓肿肝脏中分离出的两株菌株(FN37 和 FN38),它们含有多种抗生素耐药基因:cfr(C)、tet(O)、ant(6)-Ia 和 erm(B),后者通过修饰核糖体而产生对大环内酯类药物的耐药性。为了评估erm(B)是否会带来表型优势,我们测试了四个菌株(存放菌株ATCC 25286、瘤胃分离物FNC、脓肿分离物FN37和FN38)对泰乐菌素的反应。两株erm(B)-harboring菌株在瘤胃中常见的浓度下表现出抗药性,而在FN38的情况下,可耐受高达100 μg/mL的泰乐菌素。所有四种菌株对泰乐菌素的敏感性因生长阶段(静止期与对数期)和接种物的预处理(在含有浓度为 1 μg/mL 泰乐菌素的培养基中生长)而异,但携带 erm(B) 基因的两种菌株表现出很强的抗性。这一发现以及全基因组测序和初步注释表明了抗性基因的水平基因转移和获得,突出了重新审视饲养场养牛业抗菌策略的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Detection of Tylosin Resistance in Fusobacterium necrophorum subspecies necrophorum

In-feed tylosin, a macrolide, is widely used to prevent liver abscessation in feedlot cattle by repressing growth of ruminal Fusobacterium necrophorum. Although tylosin has been used for almost five decades, no resistant F. necrophorum subsp. necrophorum strain has ever been isolated. Here, we report two strains (FN37 and FN38) previously isolated from abscessed livers containing several antibiotic resistance genes: cfr(C), tet(O), ant(6)-Ia, and erm(B), the latter of which confers resistance to macrolides via modification of the ribosome. To evaluate if erm(B) conferred a phenotypic advantage, four strains (deposited strain ATCC 25286, ruminal isolate FNC, and abscess isolates FN37 and FN38) were tested for their responses to tylosin. The two erm(B)-harboring strains showed resistance at concentrations commonly found within the ruminal compartment under current dosing guidelines, and in the case of FN38, up to 100 μg/mL tylosin was tolerated. Tylosin susceptibility varied depending on the growth phase (stationary vs logarithmic) and preconditioning (growth in medium containing tylosin at a concentration of 1 μg/mL) of the inoculum in all four strains, but the two harboring the erm(B) gene demonstrated robust resistance. This discovery along with whole genome sequencing and preliminary annotation indicates horizontal gene transfer and acquisition of resistance genes, highlighting the need to revisit antimicrobial strategies for the feedlot cattle industry.

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