Cory Schwarz, Jacques Mathieu*, Jenny Laverde Gomez, Marina Tikhonova, T. G. Nagaraja and Pedro J. J. Alvarez,
{"title":"检测坏死杆菌亚种对泰乐菌素的抗药性","authors":"Cory Schwarz, Jacques Mathieu*, Jenny Laverde Gomez, Marina Tikhonova, T. G. Nagaraja and Pedro J. J. Alvarez, ","doi":"10.1021/acsagscitech.4c00159","DOIUrl":null,"url":null,"abstract":"<p >In-feed tylosin, a macrolide, is widely used to prevent liver abscessation in feedlot cattle by repressing growth of ruminal <i>Fusobacterium necrophorum</i>. Although tylosin has been used for almost five decades, no resistant <i>F. necrophorum</i> subsp. <i>necrophorum</i> strain has ever been isolated. Here, we report two strains (FN37 and FN38) previously isolated from abscessed livers containing several antibiotic resistance genes: <i>cfr</i>(C), <i>tet</i>(O), <i>ant</i>(6)-Ia, and <i>erm</i>(B), the latter of which confers resistance to macrolides via modification of the ribosome. To evaluate if <i>erm</i>(B) conferred a phenotypic advantage, four strains (deposited strain ATCC 25286, ruminal isolate FNC, and abscess isolates FN37 and FN38) were tested for their responses to tylosin. The two <i>erm</i>(B)-harboring strains showed resistance at concentrations commonly found within the ruminal compartment under current dosing guidelines, and in the case of FN38, up to 100 μg/mL tylosin was tolerated. Tylosin susceptibility varied depending on the growth phase (stationary vs logarithmic) and preconditioning (growth in medium containing tylosin at a concentration of 1 μg/mL) of the inoculum in all four strains, but the two harboring the <i>erm</i>(B) gene demonstrated robust resistance. This discovery along with whole genome sequencing and preliminary annotation indicates horizontal gene transfer and acquisition of resistance genes, highlighting the need to revisit antimicrobial strategies for the feedlot cattle industry.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 7","pages":"673–680"},"PeriodicalIF":2.3000,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Tylosin Resistance in Fusobacterium necrophorum subspecies necrophorum\",\"authors\":\"Cory Schwarz, Jacques Mathieu*, Jenny Laverde Gomez, Marina Tikhonova, T. G. Nagaraja and Pedro J. J. Alvarez, \",\"doi\":\"10.1021/acsagscitech.4c00159\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >In-feed tylosin, a macrolide, is widely used to prevent liver abscessation in feedlot cattle by repressing growth of ruminal <i>Fusobacterium necrophorum</i>. Although tylosin has been used for almost five decades, no resistant <i>F. necrophorum</i> subsp. <i>necrophorum</i> strain has ever been isolated. Here, we report two strains (FN37 and FN38) previously isolated from abscessed livers containing several antibiotic resistance genes: <i>cfr</i>(C), <i>tet</i>(O), <i>ant</i>(6)-Ia, and <i>erm</i>(B), the latter of which confers resistance to macrolides via modification of the ribosome. To evaluate if <i>erm</i>(B) conferred a phenotypic advantage, four strains (deposited strain ATCC 25286, ruminal isolate FNC, and abscess isolates FN37 and FN38) were tested for their responses to tylosin. The two <i>erm</i>(B)-harboring strains showed resistance at concentrations commonly found within the ruminal compartment under current dosing guidelines, and in the case of FN38, up to 100 μg/mL tylosin was tolerated. Tylosin susceptibility varied depending on the growth phase (stationary vs logarithmic) and preconditioning (growth in medium containing tylosin at a concentration of 1 μg/mL) of the inoculum in all four strains, but the two harboring the <i>erm</i>(B) gene demonstrated robust resistance. This discovery along with whole genome sequencing and preliminary annotation indicates horizontal gene transfer and acquisition of resistance genes, highlighting the need to revisit antimicrobial strategies for the feedlot cattle industry.</p>\",\"PeriodicalId\":93846,\"journal\":{\"name\":\"ACS agricultural science & technology\",\"volume\":\"4 7\",\"pages\":\"673–680\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-06-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS agricultural science & technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acsagscitech.4c00159\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS agricultural science & technology","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsagscitech.4c00159","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Detection of Tylosin Resistance in Fusobacterium necrophorum subspecies necrophorum
In-feed tylosin, a macrolide, is widely used to prevent liver abscessation in feedlot cattle by repressing growth of ruminal Fusobacterium necrophorum. Although tylosin has been used for almost five decades, no resistant F. necrophorum subsp. necrophorum strain has ever been isolated. Here, we report two strains (FN37 and FN38) previously isolated from abscessed livers containing several antibiotic resistance genes: cfr(C), tet(O), ant(6)-Ia, and erm(B), the latter of which confers resistance to macrolides via modification of the ribosome. To evaluate if erm(B) conferred a phenotypic advantage, four strains (deposited strain ATCC 25286, ruminal isolate FNC, and abscess isolates FN37 and FN38) were tested for their responses to tylosin. The two erm(B)-harboring strains showed resistance at concentrations commonly found within the ruminal compartment under current dosing guidelines, and in the case of FN38, up to 100 μg/mL tylosin was tolerated. Tylosin susceptibility varied depending on the growth phase (stationary vs logarithmic) and preconditioning (growth in medium containing tylosin at a concentration of 1 μg/mL) of the inoculum in all four strains, but the two harboring the erm(B) gene demonstrated robust resistance. This discovery along with whole genome sequencing and preliminary annotation indicates horizontal gene transfer and acquisition of resistance genes, highlighting the need to revisit antimicrobial strategies for the feedlot cattle industry.