R. V. Ryabokon, V. V. Tsukanov, V. Khorzhevskii, A. V. Vasyutin, J. L. Tonkikh
{"title":"萎缩性胃炎患者上皮细胞增殖指数取决于胃窦部是否存在完全或不完全肠化生","authors":"R. V. Ryabokon, V. V. Tsukanov, V. Khorzhevskii, A. V. Vasyutin, J. L. Tonkikh","doi":"10.21518/ms2024-199","DOIUrl":null,"url":null,"abstract":"Introduction. There is a debate about the significance of intestinal metaplasia (IM) subtypes for the development of gastric cancer. Therefore, determining the indicators of cellular renewal in individuals with complete and incomplete IM is certainly a topical issue.Aim. To study the proliferative activity of epithelial cells of the gastric antrum in patients with Helicobacter pylori-positive antral atrophic gastritis depending on the subtype of IM.Materials and methods. The study included 20 people with chronic antral non-atrophic gastritis (CNG; group A), 20 patients with chronic antral atrophic gastritis (CAG) without IM (group B), 20 patients with CAG with complete IM (group C) and 20 people with CAG with incomplete IM (group D). The stage of chronic gastritis was assessed by the morphological method in accordance with the modified Sydney classification. Typing of IM foci in the gastric mucosa was performed using the PAS reaction. Proliferation activity was studied by the expression of nuclear protein Ki67 using immunohistochemistry.Results. The proliferation index in the foci of complete BM in group C was 5%, and in group D in the foci of incomplete BM the Ki67 expression index was significantly higher and was 39% (p < 0.001). Outside the foci of metaplasia, the proliferation index was 23.5% in group C and 19% in group D (p = 0.06).Conclusion. We have registered significantly higher proliferation indicators of gastric epithelial cells in foci with incomplete IM compared to foci with complete IM. Determination of proliferation indicators in foci of incomplete intestinal metaplasia may be a marker of an increased risk of developing gastric cancer.","PeriodicalId":18391,"journal":{"name":"Meditsinskiy sovet = Medical Council","volume":"50 13","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Epithelial cell proliferation index in patients with atrophic gastritis depending on the presence of complete or incomplete intestinal metaplasia in the gastric antrum\",\"authors\":\"R. V. Ryabokon, V. V. Tsukanov, V. Khorzhevskii, A. V. Vasyutin, J. L. Tonkikh\",\"doi\":\"10.21518/ms2024-199\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction. There is a debate about the significance of intestinal metaplasia (IM) subtypes for the development of gastric cancer. Therefore, determining the indicators of cellular renewal in individuals with complete and incomplete IM is certainly a topical issue.Aim. To study the proliferative activity of epithelial cells of the gastric antrum in patients with Helicobacter pylori-positive antral atrophic gastritis depending on the subtype of IM.Materials and methods. The study included 20 people with chronic antral non-atrophic gastritis (CNG; group A), 20 patients with chronic antral atrophic gastritis (CAG) without IM (group B), 20 patients with CAG with complete IM (group C) and 20 people with CAG with incomplete IM (group D). The stage of chronic gastritis was assessed by the morphological method in accordance with the modified Sydney classification. Typing of IM foci in the gastric mucosa was performed using the PAS reaction. Proliferation activity was studied by the expression of nuclear protein Ki67 using immunohistochemistry.Results. The proliferation index in the foci of complete BM in group C was 5%, and in group D in the foci of incomplete BM the Ki67 expression index was significantly higher and was 39% (p < 0.001). Outside the foci of metaplasia, the proliferation index was 23.5% in group C and 19% in group D (p = 0.06).Conclusion. We have registered significantly higher proliferation indicators of gastric epithelial cells in foci with incomplete IM compared to foci with complete IM. 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引用次数: 0
摘要
导言。关于肠化生(IM)亚型对胃癌发展的重要意义存在争议。因此,确定完全性和不完全性肠化生患者的细胞更新指标无疑是一个热门话题。研究幽门螺杆菌阳性前胃萎缩性胃炎患者胃窦上皮细胞的增殖活性,具体取决于 IM 的亚型。研究包括 20 名慢性前胃非萎缩性胃炎(CNG;A 组)患者、20 名无 IM 的慢性前胃萎缩性胃炎(CAG)患者(B 组)、20 名完全 IM 的 CAG 患者(C 组)和 20 名不完全 IM 的 CAG 患者(D 组)。慢性胃炎的分期根据改良悉尼分类法的形态学方法进行评估。使用 PAS 反应对胃黏膜中的 IM 病灶进行分型。免疫组化法通过核蛋白 Ki67 的表达研究增殖活性。C 组完全基底细胞病灶的增殖指数为 5%,而 D 组不完全基底细胞病灶的 Ki67 表达指数明显更高,为 39% (p < 0.001)。在癌变灶外,C 组的增殖指数为 23.5%,D 组为 19%(P = 0.06)。我们发现不完全变性灶的胃上皮细胞增殖指数明显高于完全变性灶。不完全性肠化生病灶中增殖指标的测定可能是胃癌发病风险增加的一个标志。
Epithelial cell proliferation index in patients with atrophic gastritis depending on the presence of complete or incomplete intestinal metaplasia in the gastric antrum
Introduction. There is a debate about the significance of intestinal metaplasia (IM) subtypes for the development of gastric cancer. Therefore, determining the indicators of cellular renewal in individuals with complete and incomplete IM is certainly a topical issue.Aim. To study the proliferative activity of epithelial cells of the gastric antrum in patients with Helicobacter pylori-positive antral atrophic gastritis depending on the subtype of IM.Materials and methods. The study included 20 people with chronic antral non-atrophic gastritis (CNG; group A), 20 patients with chronic antral atrophic gastritis (CAG) without IM (group B), 20 patients with CAG with complete IM (group C) and 20 people with CAG with incomplete IM (group D). The stage of chronic gastritis was assessed by the morphological method in accordance with the modified Sydney classification. Typing of IM foci in the gastric mucosa was performed using the PAS reaction. Proliferation activity was studied by the expression of nuclear protein Ki67 using immunohistochemistry.Results. The proliferation index in the foci of complete BM in group C was 5%, and in group D in the foci of incomplete BM the Ki67 expression index was significantly higher and was 39% (p < 0.001). Outside the foci of metaplasia, the proliferation index was 23.5% in group C and 19% in group D (p = 0.06).Conclusion. We have registered significantly higher proliferation indicators of gastric epithelial cells in foci with incomplete IM compared to foci with complete IM. Determination of proliferation indicators in foci of incomplete intestinal metaplasia may be a marker of an increased risk of developing gastric cancer.