Xinyue Jia, Jiayang He, Mengdie Li, Jingjing Ye, Yuqing Zhang, Chunli Yang, Ruo Yuan* and Wenju Xu*,
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Benefitting from the self-enhanced effect of multiple emitters, this label-free fluorescent sensing strategy features simplicity, rapidity, and high on–off contrast, without involving complicated nucleic acid amplifiers.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":6.7000,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Stimulus-Responsive Four-Stranded DNA Nanoring Assembly to Host Multiple Nanosilver Clusters for Cooperatively Enhanced Fluorescence Biosensing\",\"authors\":\"Xinyue Jia, Jiayang He, Mengdie Li, Jingjing Ye, Yuqing Zhang, Chunli Yang, Ruo Yuan* and Wenju Xu*, \",\"doi\":\"10.1021/acs.analchem.4c01538\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Exploring the ability of four-stranded DNA nanorings (<i>fs</i>DNRs) to host multiple nanosilver clusters (NAgCs) for cooperatively amplifiable fluorescence biosensing to a specific initiator (<i>t</i><b>I*</b>) is fascinating. 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引用次数: 0
摘要
探索四链 DNA 纳米环(fsDNR)承载多个纳米银团簇(NAgCs)的能力,以实现对特定引发剂(tI*)的可协同放大的荧光生物传感,是一项引人入胜的研究。我们设计了三条 DNA 单链和三个类似的茎环发夹,通过顺序交叉打开和重叠杂交,开发出了功能性 fsDNR。需要注意的是,一条底物链(SS)被编程为六个模块:NAgCs 模板的两个分离片段(sT 和 sT')、中间非配对间隔的两个隔离片段以及一个可识别 tI* 位移的分区,而 sT 和 sT' 也被系在三个发夹的两端。首先,形成了一个具有刺激响应性的三重 dsDNA 复合物,以引导与 tI* 的特异性结合,而暴露在外的 SS 脚趾则激活了三个发夹的正向级联杂交,直至在定制的自组装途径中闭环,形成 fsDNR。由此产生的四个双链体迫使每对 sT/sT' 作为母模板在四个缺口处合并,引导共享 fsDNR 中的四个簇优先合成,从而协同放大绿色荧光信号,实现对 tI* 的灵敏检测。同时,fsDNR 的拓扑构象可通过已形成的簇加合物稳定下来,从而铆接住裂口中的一对两裂口。得益于多个发射器的自增强效应,这种无标记荧光传感策略具有简单、快速和高开关对比度的特点,而且不涉及复杂的核酸放大器。
Stimulus-Responsive Four-Stranded DNA Nanoring Assembly to Host Multiple Nanosilver Clusters for Cooperatively Enhanced Fluorescence Biosensing
Exploring the ability of four-stranded DNA nanorings (fsDNRs) to host multiple nanosilver clusters (NAgCs) for cooperatively amplifiable fluorescence biosensing to a specific initiator (tI*) is fascinating. By designing three DNA single strands and three analogous stem-loop hairpins, we developed a functional fsDNR through sequential cross-opening and overlapped hybridization. Note that a substrate strand (SS) was programmed with six modules: two severed splits (sT and sT′) of NAgCs template, two sequestered segments by a middle unpaired spacer, and a partition for tI*-recognizable displacement, while sT and sT′ were also tethered in two ends of three hairpins. At first, a triple dsDNA complex with stimulus-responsiveness was formed to guide the specific binding to tI*, while the exposed toehold of the SS activated the forward cascade hybridization of three hairpins, until the ring closure in the tailored self-assembly pathway for forming the fsDNR. The resulting four duplexes forced each pair of sT/sT′ to be merged as the parent template in four nicks, guiding the preferential synthesis of four clusters in the shared fsDNR, thereby cooperatively amplifying the green fluorescence signal for sensitive assay of tI*. Meanwhile, the topological conformation of fsDNR can be stabilized by the as-formed cluster adducts to rivet the pair of two splits in the nicks. Benefitting from the self-enhanced effect of multiple emitters, this label-free fluorescent sensing strategy features simplicity, rapidity, and high on–off contrast, without involving complicated nucleic acid amplifiers.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.